Effects of magnetic hydroethanolic extract of Nigella sativa and magnesium oxide nanoparticles (MgO) on catalase gene expression in liver of diabetic male rats

Background: Diabetes is a metabolic disorder with chemical, physiological and pathological changes in the liver. Due to the side effects of insulin, the use of herbal medicines such as Nigella sativa with flavonoid compounds and antioxidant properties is considered. In this study, the effect of magnetic hydroethanolic extract of Nigella sativa and MgO nanoparticles separately and simultaneously on the expression of CAT gene in liver tissue of male diabetic rats was investigated. Materials and methods: 40 male Wistar rats, after induction of diabetes by injection of streptozotomycin (STZ) at a dose of 60 mg / kg, were treated by magnetic hydroathanolic extract of Nigella sativa at a dose of 250 mg / kg, MgO nanoparticles at a dose of 10 mg / kg, combined magnetic hydroathanolic extract 125 mg / kg and 5 mg / kg MgO nanoparticles, and the extract loaded with MgO nanoparticles every other day for 28 days and the expression of liver CAT gene was evaluated. Results: The extract loaded with MgO nanoparticles was able to increase CAT gene expression in diabetics to the extent of gene expression in metformin-treated diabetics. Conclusion: It seems that thymoquinone (TQ) in Nigella sativa due to its antioxidant properties increases the expression of CAT gene and overcomes the oxidative stress caused by diabetes with its protective effect on pancreatic beta cells. Loading the nanoparticles probably resulted in better absorption of the antioxidant compounds in the cell, and the MgO nanoparticles as nanocontractors facilitated the cell's access to the particulate compounds.