Effects of Different Doses of Lif on Ivm Rate and Cumulus Expansion In Mouse Oocytes
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چکیده مقاله:
Purpose: The aim of this research was to study the effects of different doses of LIF on GVBD and MII development rate and cumulus expansion. Materials and Methods: Immature mice superovulated with HMG and GV oocytes obtained from ovary 48 hours later. The GV oocytes were cultured in TCM199 with 100, 500 and 1000 IU /ml LIF. Cumulus expansions were evaluated with two examiners and numbers of MII oocytes were recorded. For denuding the oocytes hyaloronidase was used.Results: Our results showed that the rate of GVBD and MII development increased in groups with LIF compared with control group. Rate of MII development with 1000 IU/ml LIF was significantly higherthan that of control group (P<0.05). Cumulus expansion in group with 1000 IU/ml LIF improved significantly compared with control group (p<0.05). Conclusion: Our results showed that LIF could improve IVM rate in dose dependant. Also cumulus expansion improved in group with LIF and increased oocyte quality.
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عنوان ژورنال
دوره 7 شماره 27
صفحات 0- 0
تاریخ انتشار 2009-07
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