Effect of intracerebroventricular injection of COX-1 inhibitor (ketoprofen) on PTZ-induced seizures in male rat

Introduction: Ketoprofen is an NSAID and selective COX-1 inhibitor. In our previous study the role of flunixin meglumine, a nonselective COX inhibitor was studied on seizure and its anticonvulsant effects were confirmed. Therefore this research is performed to assess the role of a selective COX-1 inhibitor, ketoprofen in treatment of seizures induced by PTZ. Methods: In this research, male Wistar rats (200±20 g) were given intracerebroventricular injections (1μl volume in each), of saline or ketoprofen (25 μg, 50 μg and 100 μg) before intraperitoneal administration of PTZ (80 mg/kg) for induction of seizure. Then, seizure score and times of onset of every stage of seizure were recorded during 20 minutes after PTZ administration. The data was analyzed by one-way analysis of variance (ANOVA) and nonparametric tests. Results: Result of this research indicated that the injection of 50 μg of ketoprofen significantly increased the time of onset of partial seizure compared to the control group. Also ketoprofen with doses of 50 and 100 μg significantly increased the time of onset of generalized (tonic-clonic) seizures compared to the control group. On the other hand, 25 μg of ketoprofen did not have a significant effect in comparison with the control group. Fifty μg of ketoprofen decreased the seizure score compared to the control, but this decrease did not reach significance (P>0.05). Conclusion: We conclude that ketoprofen has anticonvulsive properties.