Effect of 8 weeks high intensity interval training with sodium citrate supplementation on PGC-1α and TFAM expression

Abstract Introduction & Objective: High intensity interval training can promote PGC-1α and TFAM key regulator of mitochondrial biogenesis. Then the purpose of this investigation is to examine the effect of high intensity interval training whit supplementation of sodium citrate on PGC-1α and TFAM expression in soleus muscle of Wistar rats. Methodology: 24 Wistar rats were randomly divided into 3 groups of control, training and training with sodium citrate supplementation. Both training and training with sodium citrate supplementation groups performed high intensity interval training for 8 weeks, five days a week, with the intensity of 50% to 90% maximum running speed on rodent treadmill. The training with supplement group were treated with a dose (15 mmol/L) of sodium citrate by drinking water per day for 8 weeks. Statistical evaluations of the experiments were investigated by applying One-Way ANOVA and Tukey’s post hoc analysis with a statistical level of p <0.05. Cohen’s d also was calculated to compare groups. Results: Findings indicated significant differences in PGC-1α and TFAM protein level in both training and training with supplement groups compared to control group (p=0.001). Results about sodium citrate supplementation also showed significant differences in training with supplementation group compared to training group (p=0.005). Conclusion: It seems sodium citrate supplementation can induce expression of PGC-1α and TFAM and increases level of PGC-1α and TFAM proteins.