Association the study of between CGA rs6631A>T gene polymorphism with the risk of male infertility

 Introduction: The CGA gene encodes alpha subunit of glycoprotein hormones that are involved in the fertility process. The aim of this study was to evaluate the relationship between glycoprotein hormones, alpha polypeptide (CGA)  rs6631A> T gene polymorphism with the risk of men with azoospermia or severe oligozoospermia. Methods:  This study was conducted in a case-control study on 200 blood samples consisting of 100 samples of severe oligozoospermia or azoospermia men and 100 blood samples of healthy men with normal fertility and lack of a family history of infertility. In this study, we were used PCR-RFLP method to evaluate genotype frequencies of CGA polymorphism.  Results:  The genotype frequencies of CGA gene polymorphism for AA, TA and TT was 27%, 51% and 22% and 24%, 47% and 27%, in control and case groups, respectively. There was no significant difference in genotype frequencies between both groups (P> 0.05). In addition, there was no significant difference in allele frequency between two groups (P=0.424, OR=0.852, CI=0.576-1.261). Conclusion: The results of this investigation showed that nucleotide change in CGA gene was not significantly associated with the risk of azoospermia or severe oligozoospermia; therefore, this polymorphism may not be a prognostic biomarker in male infertility.