Association of the Polymorphism Kiss1 Gene Exon 1 with Twin Traits in Sanjabi, Ghezel Breed Sheep by PCR–SSCP Technique

The Kiss1 gene is one of the known affective gene in fertility, which affects ovulation and multiplication. In this study, Blood samples were collected from 100 sheep of Sanjabi in Mehregan breeding station in Kermanshah province and 50 sheep Ghezel breeding station of Urmia University. After DNA extraction, the polymerase chain reaction (PCR) was used for specific primers amplification of 331 bp fragment of Exon1 Kiss1 gene. Then single strand conformation polymorphism (SSCP) of PCR products was performed and genotypic patterns were obtained using acrylamid gel and the gel stain was obtained by silver nitrate method. The results showed different patterns that could be due to the presence of polymorphism in this position. Three different genotype patterns in samples 1, 2, 3, 4 and 5 for Sanjabi breed  46% ,23% ,3%, 17% and 11% respectively and two different genotype pattern The frequency were 1and 2 for Ghezel breed 0.24% and 0.76%. The association of observed patterns on the multiplicity trait was significant in Sanjabi breed (P <0.05). But this relationship was not in was significant in Ghezel breeds. The results of this study showed that the variation in exon one of the Kiss1 gene can be considered in marker assistance selection programs in sanjabi sheep breed.