Anti-hepatofibrotic effect of ethyl acetate fraction of Bombax costatum Pellgr.EtVuillet stem bark against CCl4-induced liver fibrosis in mice

نویسندگان

  • Aliyu Musa Department of Pharmaceutical and Medicinal Chemistry, Ahmadu Bello University Zaria, Nigeria;
  • Jamilu Yau Department of pharmacology and therapeutics, Faculty of pharmaceutical sciences, Ahmadu Bello University, Zaria, Nigeria.
  • Joseph Anuka Department of pharmacology and therapeutics, Faculty of pharmaceutical sciences, Ahmadu Bello University, Zaria, Nigeria.
  • Nuhu Mohammed Department of pharmacology and therapeutics, Faculty of pharmaceutical sciences, Ahmadu Bello University, Zaria, Nigeria.
چکیده مقاله:

Background & Aim: Bombax costatum stem bark is traditionally used in treatment of liver diseases but the anti-hepatofibrotic effect of its ethyl acetate fraction has not been scientifically evaluated. This study aimed to evaluate the anti-hepatofibrotic effect of ethyl acetate fraction of B. costatum stem bark (EAB) against carbon tetrachloride (CCl4) induced liver fibrosis in mice. Experimental: Normal control group were administered olive oil while mice in the remaining groups received 0.4 ml/kg of CCl4 twice weekly for 6 weeks. Mice in treatment groups received EAB once daily orally for the next 2 weeks at doses of 31.25, 62.5 and 125 mg/kg body weight, respectively. Mice in standard control group received silymarin (100 mg/kg) daily for the next 2 weeks. Mice in toxic control group were sacrificed 72 hours after the last dose of CCl4 while mice in CCl4 control group were observed for the next 2 weeks for spontaneous resolution of fibrosis. Effect of treatment on tumor necrosis factor-α (TNFα), transforming growth factor-β1 (TGFβ1), malondialdehyde (MDA), reduced glutathione (GSH) levels and liver histopathology were evaluated. Results: CCl4 intoxication caused significant (p < 0.05) elevation in TNFα, TGFβ1 and MDA with associated collagen deposition in the liver. Administration of EAB significantly (p < 0.05) decreased the level of TNFα, TGFβ1 as well as MDA and increased GSH level compared to CCl4 toxic group. Recommended applications/industries: Ethyl acetate fraction of B. costatum possesses anti-hepatofibrotic, anti-inflammatory and in vivo anti-oxidant activities against CCl4 induced liver fibrosis.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Improving Effect of Ethyl Acetate Fraction of Tanacetum Parthenium Against Brain Oxidative Damage in Pentylenetetrazole-Induced Seizure Model In Mice

Aims: Oxidative stress plays an important role in pathogenesis of seizure. In this study the effect of ethyl acetate fraction of Tanacetum parthenium against brain oxidative damage in pentylenetetrazole-induced (PTZ) seizure model in mice was investigated. Materials and methods: In this experimental study, mice were distributed into 6 groups: 1) Control, 2) PTZ, 3-6) Fraction+PTZ. The animals ...

متن کامل

Protective Effect of Wrightia Tinctoria Bark Triterpenoidal Fraction on Ccl4-Induced Acute Rat Liver Toxicity

The present investigation aims at assessing the hepatoprotective effect of triterpene fraction isolated from the stem bark of Wrightia tinctoria (containing lupeol, b -amyrin and b -sitosterol) on CCl 4 -induced hepatotoxicity in the rat. CCl 4 (1.5 mg/kg, i.p) is a potent hepatotoxic agent which causes peroxidative degeneration of membrane lipids with the potential outcome of fatt...

متن کامل

Therapeutic Potential of Mouse Bone Marrow Mesenchymal Stem Cells in Carbon Tetrachloride (Ccl4)-Induced Liver Fibrosis

Purpose: To study the effect of allogenic bone marrow mesenchymal stem cells (BMMSCs) transplantation on carbon tetrachloride-induced liver fibrosis in mice. Materials and Methods: Fifty five female NMRI mice were divided in 5 groups, and to induce liver fibrosis CCL4 intraperitonealy was injected 1ml/Kg twice a week for 8 weeks 106 allogenic BMMSCs were infused in cell therapy group via tail v...

متن کامل

Hepatoprotective Activity of Capparis spinosa Root Bark Against CCl4 Induced Hepatic Damage in Mice

Many hepatoprotective herbal preparations have been recommended in alternative systems of medicine for the treatment of hepatic disorders. No systematic study has been done on protective efficacy of Capparis spinosa (Capparidaceae) to treat hepatic diseases. Protective action of C. spinosa ethanolic root bark extract was evaluated in this study in an animal model of hepatotoxicity, which was in...

متن کامل

Hepatoprotective Activity of Capparis spinosa Root Bark Against CCl4 Induced Hepatic Damage in Mice

Many hepatoprotective herbal preparations have been recommended in alternative systems of medicine for the treatment of hepatic disorders. No systematic study has been done on protective efficacy of Capparis spinosa (Capparidaceae) to treat hepatic diseases. Protective action of C. spinosa ethanolic root bark extract was evaluated in this study in an animal model of hepatotoxicity, which was in...

متن کامل

protective effect of wrightia tinctoria bark triterpenoidal fraction on ccl4-induced acute rat liver toxicity

the present investigation aims at assessing the hepatoprotective effect of triterpene fraction isolated from the stem bark of wrightia tinctoria (containing lupeol, b -amyrin and b -sitosterol) on ccl 4 -induced hepatotoxicity in the rat. ccl 4 (1.5 mg/kg, i.p) is a potent hepatotoxic agent which causes peroxidative degeneration of membrane lipids with the potential outcome of fatty degeneratio...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 11  شماره 1

صفحات  1- 7

تاریخ انتشار 2020-12-21

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023