کلونینگ cDNA فاکتور VII انعقادی حاصل از رده سلولی هپاتوما

Abstract Background: Factor VII, is a coagulant protease it begins the proteolytic cascade reactions and produces thrombin. The use of recombinant human factor VII, (rhFVII) is effective for the treatment of patients with hemophilia A or B. It is a target for gene therapy. This study was done to clone factor VII from HepG2 cell line. Methods: RNA was extracted from the hepatoma, (HepG2), cell line. On reverse transcription FVII cDNA was amplified by RT-PCR. PCR product was cloned into the pTZ57R/T vector and transported into the E-coli cells. Results: By amplification of the FVII gene, the PCR band was observed and cloning into the vector was confirmed by restriction analysis. Conclusion: In this paper we report the cloning of factor VII from HepG2 cell line. Keywords: Cloning, Coagulant VII factor, Hepatoma cell line.