ردیابی ژن‌های انتروتوکسین A وB باکتری استافیلوکوکوس اورئوس در نمونه‌های بالینی بیماران مراجعه‌کننده به مراکز درمانی شهرستان کرمان و رفسنجان با روش ملکولی

  Background and Objectives : Staphylococcus aureus is the positive gram coccus which is able to cause different kinds of infection in certain condition. The function of this bacteria is to provide the conditions for the invasion of it to the host with the secretion of different sorts of toxins such as entrotoxin. A and B entrotoxins have the most infections effect among these toxins. that’s why, this experiment for the has been conducted for the detection of entrotoxin genes A and B in clinical samples.   Materials and Method: This cross-sectional and descriptive study, which was conducted from September 2007 to August 2008. Two handred and forty clinical samples from wound, wound, blood, ear, nose, vomited secretions and urine were separation investigated (forty from each sample) and taken from the patients. then after the culture of the samples and conducting confirming biochemical tests, then they were assessed by Poly Chain Reaction technique.   Results: After the diagnosis of this bacteria and conducting objective test, from two hundred and forty samples examined throughout this study, fifty ones (20.83%) were infected by Staphylococcus aureus. Thirty seven cases (74%) of these infected samples were carrying both entrotoxin A and B genes. The frequencies of A and B genes were 11(22%) and 2 (4%), respectively.   Discussion: This study identified that 20.83% of the patients were carrying the pathogen as the source of infection. The detection of entrotoxin A and B genes, shows the most important role they have in bringing about superinfection.   Key words: Staphylococcus Aureus, Gene, Enterotoxins, PCR     Funding: This research was funded by Islamic Azad University Qom Branch.   Conflict of Interest: Not declared.   Ethical approval: The Ethics Committee of Islamic Azad University Qom Branch approved the study.    How to cite this article : Salari Sharif A, Sattari M, Moradi M, Shahrokhabad R . Detection of Staphylococcus aureus Entrotoxin Genes A & B in Clinical Samples of the Patients Referring to the Medical Centers of Kerman and Rafsanjan Cities by PCR Technique . J Rafsanjan Univ Med Scie 2012 11(2): 128-36. [Farsi]