تولید آنتی‌بادی پلی‌کلونال شتری علیه گیرنده 2 فاکتور رشد سلول‌های آندوتلیال عروق و بررسی عملکرد آن

Abstract Background: In molecular approach, serum of camel contains a unique type of antibodies devoid of light chains since the light chain is missing, the heavy-chain antibodies should bind their antigen by one single domain, the variable domain of the heavy immunoglobulin chain. Vascular endothelial growth factor receptor-2 (VEGFR-2) is one of the important proteins in angiogenesis which over-expressed in many types of tumors. Methods: Two male dromedaries (Camelus dromedarius) were immunized against VEGFR-2. ELISA was used to evaluate the immunization process. Camel immune sera were fractionated with protein A and G affinity chromatography. Heavy chain antibodies tested for its specific reactivity in cell-based ELISA in recognition of VEGFR-2 on the cell surface of three cell lines 293/KDR, HUVECs and A431. Results: In ELISA test, the camel sera in 1/12800 dilution had the acceptable results. Furthermore, cell-based ELISA demonstrated that the polyclonal heavy chain antibodies bind to VEGFR-2 in 293/KDR and HUVECs cell lines.Conclusion: Single chain polyclonal antibodies against VEGFR-2 can be used for detection of soluble form of this protein by ELISA, and also flowcytometry, western blotting and immunohistochemistry. Keywords: Camel, Single chain antibody, Polyclonal antibody, VEGFR-2.