تغییرات وابسته به دوز عصاره هیدروالکلی کلم بروکلی در کنترل استرس اکسیداتیو القایی دیازینون بر سلول‌های بافت بیضه موش صحرایی نر بالغ

Background and Objectives: Diazinon is one of the organophosphorus poisons that is widely used as insecticide and causes male infertility. The aim of this study was to evaluate the effects of broccoli hydroalcholic extract on the changes of the cells of testicular tissue and the control of oxidative stress by diazinon. Materials and Methods: In this experimental research, 30 adult male Wistar rats weighing 200-250 gr were used. The control group receivied no treatment and the T1 group recievied the peritoneal administration of 30 mg/kg b.w diazinon and the other experimental groups (T2, T3, and T4) received diazonin as well as broccoli extract at dosage of 100, 200, and 300 mg/kg b.w, respectively for 28 days. At the end of the study, the animals were weighed and euthanized. The right testicle was used for histological study and the left testis for measuring malondialdehyde. The data was analyzed using one-way ANOVA and Tukey's post hoc test. Results: The lowest mean of body weight was 279.763&plusmn;11.899 gr in the T1 group and the highest was 311.831&plusmn;8.976 gr in the T4 group and it was observed a statistically significant difference (P=0.038). The number of the sertoli, spermatogonia, spermatocyt, and spermatides cells were 28.751&plusmn;1.544, 60.501&plusmn;4.828, 202.503&plusmn;11.677, and 184.120&plusmn;19.593, respectively in the T4 group. The highest number avarage was in the T4 group and the lowest in the T1 group (P=0.040). The highiest malondialdehyde concentration was 3.805&plusmn; 0.017 in the T1 group and the lowest was 2.204&plusmn; 0.056 &micro;mol/ ml in the T4 group, which was significant (P<0.05). Conclusion: Broccoli extract at dosage of 300 mg/kg b.w has a positive effect on the spermatogenesis damage caused by diazinon in male rats. Key words: Oxidative stress, Brassica oleracea var. italica hydroalcholic extract, Diazinon, Testicular tissue, Rat Funding: This study was funded by Islamic Azad University of Sanandaj Branch. Conflict of interest: None declared. Ethical approval: The Ethics Committee of Sanandaj University approved the study. &nbsp; How to cite this article: Qaderi Forough M, Raeeszadeh M, Amiri A.A. Dose-Response Changes of Brassica oleracea var. italica Hydroalcholic Extract in the Control of Oxidative Stress by Induction of Diazinon on the Cells of Testicular Tissue in Male Adult Rat. J Rafsanjan Univ Med Sci 2017; 16(7): 593-604. [Farsi] &nbsp;