تأثیر محیط کشت القاء شده با سلول های فیبروبلاست بر بلوغ آزمایشگاهی تخمک های نابالغ موش

Background and purpose: The purpose of this study was to evaluate the effect of mouse embryonic fibroblast cells conditioned medium on resumption of meiosis, in vitro maturation of immature mouse oocytes, fertilization and derived embryos development. Material and Methods: Immature oocytes denuded at the germinal vesicle (GV) stage were obtained from female NMRI mice 46-48 hrs after injection of 7/5 IU PMSG. To preparate MEF, the fetuses were collected from the female mice at day 13 and cultured in DMEM. After the preparation of a monolayer, conditioned medium were collected and used for culture. Immature oocytes were randomly cultured in culture medium of MEM-α supplemented with different concentrations (0, 10, 30, 50 and 100%) of mouse embryonic fibroblast conditioned medium. After 14-16 hrs the matured oocytes were fertilized with spermatozoa in T6 medium and their development was assessed until blastocyst stage. Results: There was no significant difference in maturation rate between control and conditioned medium groups. But there were significant differences (P<0.05) in the percentage of fertilization and cleavage in conditioned medium with 30%-50% concentrations as compared to the control group (P<0.05). Conclusion: The conditioned medium of fibroblast cells increase in vitro fertilization (IVF) and embryo development. But there was no effect on the maturation of immature oocytes.