تأثیرکلرید سدیم بر فعالیت آنزیم لیپوکسی ژناز، میزان پراکسید هیدروژن پراکسیداسیون چربی در دانهال‌های سه پایه پسته

One of the main criteria for salt tolerance is cell membrane stability under stress. Reactive oxygen species (ROS), activity of lipoxygenase (Lox) and lipid peroxidation are considered to be destructive to cell membrane under salt stress. In this study, the effects of 0, 75 and 150 mM NaCI, over a period of 14 days on the activity of lipoxygenease, the amount of hydrogen peroxide (H2O2) and malon dialdehyde (MDA. as a lipid peroxidation) in the leaves of Pistacia vera L. (cv. 'Qazvini' and wild 'Sarakhs' pistachio) and 'Mastic' (P. mutica F. & M.) were studied. The results indicated that by increasing salinity Lox activity increased in the leaves of all the three rootstocks. This activity reached a climax on the 7th day and then decreased on the 14th day. Among the three species, Mastic reached the highest amount of the Lox activity on the 14th day with the lowest amount of Lox reduction. The amounts of H2O2 in the leaves of all the three rootstocks increased, on the 14th day the highest amount of hydrogen peroxide was found in 'Mastic' and 'Sarakhs' after treating them with 150 mM NaCl. The amounts of MDA were also reached the highest level in all three rootstocks on the 7th and 14th days. The results also indicated the possibility of the use of lipid peroxidation index and Lox activity for selecting salt tolerant Pistacia rootstocks. More studies are needed for understanding the biochemical changes and enzyme activities in Pistacia rootstocks under salt stress.