بیان موقت پروتئین هورمون رشد انسانی در برگ گیاه دارویی یونجه

Introduction: Human growth hormone (HGH) is very important pharmaceutical substance which is used as an absolute cure for different diseases that is why the production of HGH recombinant is taken into consideration not only nationally but also internationally. Many different methods and host systems have been used as growth hormone factories. Finding new and efficient hosts for recombinant molecules production have always played a major role in gene-expression researches. During the last decade the production of recombinant pharmaceutical proteins in plants, especially those that are pharmaceutically or nutritionally important and valuable, like alfalfa, have been considered crucial. Objective: The aim of this research was to evaluate of the potential of alfalfa, a fast growing plant for production of a recombinant protein. Materials & Methods: Transformation method was Agroinfiltration (gene transfer via Agrobacterium under vacuum condition) in which an HGH expression vector was transferred to alfalfa leaves within 6 different durations (5, 10, 15, 20, 30 and 35 minutes). The produced HGH was determined by HGH ELISA kit and western blot. Results: The best expression was gained within 30 minute vacuum for 3 days, while plants were preserved in 25C and 16 hours of 6000-8000 lux light as daylight and 8 hours of darkness photoperiod. The TSP (Total Soluble Protein) was 0.1%. Conclusion: It can be concluded that alfalfa may be used as an interesting factory for the production of recombinant proteins like pharmaceuticals and vaccines. As this method is relatively fast, easy and economical, it is one of the best systems to check and control plant and expression vector before spending resources on stable transformation.