بررسی پلی‌مورفیسم ژنهای آپولیپوپروتئین‌های AI و CIII در بیماران مبتلا به گرفتگی عروق کرونر

Several polymorphisms within the apo AI-CIII-AIV gene cluster have been detected there is ample evidence about variations at the adjucent apo AI and apo CIII gene loci influence the risk for hypertriglyceridemia and coronary artery disease(CAD). This study was conducted to elucidate the association between two restriction fragment length polymorphisms(RFLPs) of the adjacent apo AI and CIII genes and CAD in Iranian patients. The polymorphisms sites were MspI site in the promoter region of the apo AI gene(G-78A substitution) and SstI site in the apo CIII 3’ untranslated region(G 3175C substitution). Total genomic DNA was prepared from the whole blood of 46 hypertiglyceridemic patients with CAD and 36 normolipidemic Iranian subjects. Amplification of a 355 bp fragment of the promoter region of the apo AI gene and a 280 bp fragment of apo CIII 3’ untranslated region were achieved by polymerase chain reaction(PCR) with specific primers. Genotype distribution and the allele frequencies for the apo AI MspI and apo CIII SstI polymorphi sms were determined and compared in patients and normolipidemic healthy subjects. Dispite the significant differences in plasma lipids concentration between patients and healthy subjects no association between any of two investigated polymorphisms and cornary artery disease was found. This lack of association was held for both allele frequencies and genotype distribution in different sexes.