بررسی نقش پارامترهای اسپرمی و اختلالات کروماتین اسپرم با ناباروری توسط CASA و تست تفرق کروماتین

Abstract Background: Although normal morphologic and motility characteristics of sperms are necessary for fertility, normal morphology per se cannot demonstrate sperm DNA competence. Since the health of sperm DNA affects the results of assisted reproductive technology the purpose of this study is to evaluate sperm parameters measured by CASA for chromatin abnormalities. Materials and methods: Semen samples were collected from 63 cases subjects according to WHO guidelines. Semen analyses by CASA, protamine deficiency by CMA3 staining and sperm DNA fragmentation by SCD test were done. Results: The results showed a significant correlation between protamine deficiency, DNA fragmentation and sperm parameters. Head and neck anomalies showed maximum significant correlation with percentage of sperm DNA fragmentation but even tail anomalies were seen to be related to DNA competence. Conclusion: Protamine deficiency and damage in sperm DNA in infertile cases was more than fertile subjects. In cases with high DNA damage ratio, sperm parameters were poorer and fertility was impaired CASA method can be used to analyze sperm parameters with high precision. Keywords: Sperm chromatin, Infertility, CASA, Chromatin dispersion test.