بررسی ارتباط پلی مورفیسم G/C 765- ژن سیکلواکسیژناژ 2 با سرطان ریه در جمعیت اصفهان

Abstract Background: Two types of Cyclooxygenase enzymes, which produce prostaglandins, are present in humans. Prostaglandins play a role in the functions of various organs including the immune system, blood circulation, and cell division. Reports indicate that the level of these enzymes is increased in patients with metastases in several malignancies, including larynx, lung, stomach, colon, and prostate. The aim of this study was to investigate the association between -765 G/C promoter polymorphism of cyclooxygenase 2 and initiation and progression of lung cancer. Materials and methods: This is a case-control, retrospective study. Genotyping of cyclooxygenases was carried out by testing blood samples from 120 lung cancer patients and 110 controls using restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) technique. The observed numbers of each cyclooxygenase genotype were compared with that expected for a population in Hardy-Weinberg equilibrium by using a χ2 test. The significance of the differences of observed alleles and genotypes between groups was tested using the odds ratio analysis. Results: The genotype GG of polymorphism -765G/C seen in 62.5% of patients did not indicate a significant statistic increase in comparison with the control group (55.45% P< 0.6) however, when the patients were divided into two groups i.e. those with metastases and without metastases, a weak link was identified between genotype GG of polymorphism G/C, with metastasizing cancer, (P< 0.04). Dividing patients into sub-groups such as sex and smoking habit showed no difference. Conclusion: It seems that there is no association between -765 G/C polymorphism and developing of lung cancer statistically. But genotype GG of polymorphism -765G/C is linked weakly with lung cancer metastases. Keywords: Cyclooxygenases, Prostaglandin, Metastatic activity, Lung cancer.