Saman Hosseinkhani
Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
[ 1 ] - Comparison of BAX and Bcl-2 Expression During Human Embryonic Stem Cell Differentiation into Cardiomyocytes and Doxorubicin-induced Apoptosis
Back ground: Although the cell differentiation is an inseparable part of development in multicellular organisms, the regulating molecular pathway of it still is not fully defined. In the other hand, apoptosis is a fundamental physiological process which plays an essential role in a variety of biological events during development. Moreover, recent studies have found that apoptosis shows several ...
[ 2 ] - Protein Stability, Folding, Disaggregation and Etiology of Conformational Malfunctions
Estimation of protein stability is important for many reasons: first providing an understanding of the basic thermodynamics of the process of folding, protein engineering, and protein stability plays important role in biotechnology especially in food and protein drug design. Today, proteins are used in many branches, including industrial processes, pharmaceutical industry, and medical fields. A...
[ 3 ] - Simple and Rapid Immobilization of Firefly Luciferase on Functionalized Magnetic Nanoparticles; a Try to Improve Kinetic Properties and Stability
We expressed and purified a recombinant P. pyralis luciferase with N-terminal His-tags. The silanized Ni or Cu-loaded magnetic particles were prepared and used to assemble the His-tagged P. pyralis luciferase. This enzyme immobilized on functionalized magnetic nanoparticles (MNPs) via electrostatic interactions of His-tag with Ni2+/Cu2+ ions on the surface of MNPs using si...
[ 4 ] - The effect of graphite sources on preparation of Photoluminescent graphene nano-sheets for biomedical imaging
Objective(s): Graphene as two-dimensional (2D) materials have attracted wide attention in different fields such as biomedical imaging. Ultra-small graphene nano-sheets (UGNSs) have been designated as low dimensional graphene sheets with lateral dimensions less than few nanometres (≤ 500 nm) in one, two or few layers. Several studies have proven that the process of acidic exfoliation and oxidati...
[ 5 ] - Design and bioinformatics analysis of novel biomimetic peptides as nanocarriers for gene transfer
Objective(s): The introduction of nucleic acids into cells for therapeutic objectives is significantly hindered by the size and charge of these molecules and therefore requires efficient vectors that assist cellular uptake. For several years great efforts have been devoted to the study of development of recombinant vectors based on biological domains with potential applications in gene therapy....
[ 6 ] - تمایز سلول‌های بنیادی پوست انسان به سلول‌های سازنده انسولین در محیط برون‌تن با استفاده از گلوکز و عصاره پانکراس ترمیمی
سلول درمانی یکی از روش های امیدوارکننده در درمان دیابت نوع1 می باشد. یکی از مناسب ترین گزینه ها برای این منظور، سلول های پیش ساز مشتق از پوست Skin-derived precursors cells (SKP) است که پتانسیل تمایز به سلول های سازنده انسولین Insulin producing cells (IPC) را دارند. در این مطالعه سلول های پیش ساز مشتق از پوست انسان Human skin-derived precursors cells (hSKP) جدا و تکثیر شده و تحت تاثیر عصاره پانک...
[ 7 ] - Ursolic acid induces myoglobin expression and skeletal muscle remodeling in mice
Introduction: Ursolic Acid (UA) is a lipophilic triterpenoid compound, found in large amounts in apple peel. Anabolic effects of UA on the skeletal muscle and the role of this tissue as a key regulator of systematic aging aroused this question in mind whether UA might amend skeletal muscle performances such as myoglobin expression and also whether it switches skeletal muscle fibers from glyc...
[ 8 ] - Expression and Purification of the luciferase enzyme and in Vivo ATP Assay
Introduction: Gene expression and purification of luciferases from the firefly, Lampyris turkestanicus, and optimization of cellular ATP measurements were performed. Methods: cDNA encoding luciferases from Lampyris turkestanicus was transferred from pQE30 vector into pET28a expression vector and pLtu28 was built. Newly constructed vector was expressed in E. coli XL1 Blue and the recombinant l...
[ 9 ] - Effects of Nonionic Surfactants on Xanthan Gum Production: a Survey on Cellular Interactions
Background: Xanthomonas campestris is a biopolymer producing gram negative bacterium. Production of xanthan biopolymer can be affected by different extrinsic factors as well as surfactants. Hitherto, effects of nonionic surfactants on xanthan production have been studied in a limited number of articles.Objective: In the present study, nonionic surfactants...
[ 10 ] - Evaluation of Cell Penetrating Peptide Delivery System on HPV16E7 Expression in Three Types of Cell Line
Background: The poor permeability of the plasma and nuclear membranes to DNA plasmids are two major barriers for the development of these therapeutic molecules. Therefore, success in gene therapy approaches depends on the development of efficient and safe non-viral delivery systems. Objectives: The aim of this study was to investigate the in vitro delivery of plasmid DNA encoding HPV16 E7 gene...
[ 11 ] - تولید آنزیم لوسیفراز ایرانی جهش‌یافته به روش SDM با کاربرد تشخیص بالینی
مقدمه: آنزیمی که واکنش بیولومینسانس یا نشر نور را در تعدادی از موجودات کاتالیز می‌کند لوسیفراز نام دارد. از واکنش کاتالیز شده توسط این آنزیم می‌توان در بسیاری از سنجش‌های تشخیص طبی ازجمله سنجش مقدار ATP در محیط، کیت‌های تشخیصی بر پایه لوسیفراز، تعیین مقدار آلودگی میکروبی در یک محیط، ساخت بیوسنسورها و گزارشگرهای زیستی استفاده نمود. در جهت پایدارسازی آنزیم با کمک روش جهش‌زایی هدف‌دار، لوسیفراز ها...
[ 12 ] - The effect of low intensity dual frequency ultrasonic waves on the viability of the B16-F10 melanoma cell
In this study, the effect of single and dual-frequency sonication on cell death of B16-F10 melanoma cells is investigated at constant temperature. Here, 20 groups were studied. The test groups consisted of: control and sham, 40 kHz (intensity: 0.24 W/cm2), 1 MHZ (intensity: 0.5 W/cm2) and the dual frequency groups which each frequency group included seven subgroups of 30, 120, 60, 150, 300, 600...
[ 13 ] - The Effect of Temperature and Acidity on Antimicrobial Activities of Pristine MWCNTs and MWCNTs-Arg
Carbon nanotubes (CNTs) have very promising applications for inhibition of microbial growth. The aim of this study is investigation and comparison of the effect of temperature and acidity on antimicrobial activities of pristine Multiwalled Carbon nanotubes (MWCNTs) and Multiwalled Carbon nanotubes-Arginine (MWCNTs-Arg). Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Conc...