Hamideh Ofoghi
Iranian research organization for science and technology
[ 1 ] - Investigation of an Optimized Context for the Expression of GFP as a Reporter Gene in Chlamydomonas Reinhardtii
Background: Chlamydomonas reinhardtii is a novel recombinant eukaryotic expression system with many advantages including fast growth rate, rapid scalability, absence of human pathogens and the ability to fold and assemble complex proteins accurately, however, obstacle relatively low expression level necessitates optimizing foreign gene expression in this system. The Green Fluorescent Protein (G...
[ 2 ] - فاکتور رشد عصب، کاربرد بالینی و تولید پروتئین نوترکیب
پروتئینهای خانواده نوروتروفین در پستانداران شامل فاکتور رشد عصب (NGF)، فاکتور نوروتروفیک مشتق از مغز (BDNF)، نوروتروفین-3 (NT-3) و نوروتروفین-4/5 (NT-4/5) هستند که بهعنوان فاکتورهای بقای سلولهای عصبی شناخته شدهاند. فاکتور رشد عصب یکی از مهمترین سیتوکینهاست که از 118 اسید آمینه تشکیل شده است. این فاکتور در رشد و تمایز سلولهای عصبی سمپاتیک محیطی مهرهداران و نورونهای کولینرژیک بازال قدامی...
[ 3 ] - بررسی اثر زنجبیل در مقایسه با ویتامین E بر کشت آزمایشگاهی سلولهای بنیادی اسپرماتوگونی گوسفند نژاد افشاری
هدف از این پژوهش بررسی تأثیر عصاره زنجبیل و ویتامین E بر عملکرد سلولهای بنیادی اسپرماتوگونی گوسفند بود. برای این منظور غلظتهای مختلف از عصاره‌های آبی و هیدروالکی زنجبیل و ویتامین E بر زنده‌مانی، تشکیل کلونی و بیان ژن‌های مهار کننده (bcl2 و bcl2l1) و پیش برنده آپوپتوز (bax) در سلولهای بنیادی اسپرماتوگونی آزمایش شدند. سلولهای بنیادی اسپرماتوگونی از بیضه گوسفندان کشتار شده با استفاده از روش هض...
[ 4 ] - Optimization of nitrogen and phosphorus removal from meat processing wastewaters using microalgal biofilms
Background: The conventional activated sludge processes are not able to completely treat the nitrogen and phosphorus compounds of meat processing wastewaters. Microalgal biofilm, which has the advantages of easiness and inexpensiveness, can be applied as a post-treatment method. Methods: The optimal levels of micronutrients and N/P ratio were aimed in the cultivation of Scenedesmus quadricauda...
[ 5 ] - Utility of P19 Gene-Silencing Suppressor for High Level Expression of Recombinant Human Therapeutic Proteins in Plant Cells
Background: The potential of plants, as a safe and eukaryotic system, is considered in the production of recombinant therapeutic human protein today; but the expression level of heterologous proteins is limited by the post-transcriptional gene silencing (PTGS) response in this new technology. The use of viral suppressors of gene silencing can prevent PTGS and improve transient expression level ...
[ 6 ] - In vitroAnticancer Evaluation of Saponins Obtained From Spirulina platensis on MDA, HepG2, and MCF7 Cell Lines
Introduction: Microalgae are known for their bioactive compounds with potential applications as antimicrobial, antiaging, and anticancer activities. Spirulina platensis (S. platensis) is a filamentous and photosynthetic microorganism that has 25 kinds of vitamins and minerals that contain many compounds with biotic activity such as alkaloids, phenolic compounds, terpenoids, and saponin...
[ 7 ] - In vitroAnticancer Evaluation of Saponins Obtained From Spirulina platensis on MDA, HepG2, and MCF7 Cell Lines
Introduction: Microalgae are known for their bioactive compounds with potential applications as antimicrobial, antiaging, and anticancer activities. Spirulina platensis (S. platensis) is a filamentous and photosynthetic microorganism that has 25 kinds of vitamins and minerals that contain many compounds with biotic activity such as alkaloids, phenolic compounds, terpenoids, and saponin...