Javad Baharara

[ 1 ] - Cytotoxic and Pro-Apoptotic Effects of Honey Bee Venom and Chrysin on Human Ovarian Cancer Cells

Background: The anti-cancer effects of honey bee venom (BV) and chrysin might open a new window for treatment of chemo-resistant cancers. This study was designed to evaluate cytotoxic and pro-apoptotic effects of BV and chrysin on A2780cp cistplatin- resistant human ovarian cancer cells. Methods: As per the study objectives, A2780cp cells were categorized to 4 groups: 3 experiment groups (treat...

[ 2 ] - Decellularized kidney in the presence of chondroitin sulfate as a natural 3D scaffold for stem cells

Objective(s): Use of biological scaffolds and automating the cells directing process with materials such as growth factors and glycosaminoglycans (GAGs) in a certain path may have beneficial effects in tissue engineering and regenerative medicine in future. In this research, chondroitin sulfate sodium was used for impregnation of the scaffolds. It is a critical component in extracellular matrix...

[ 3 ] - Preventive Effect of Vitamin B6 on Developmental Toxicity of Carbamazepine in Mice

Objective(s) Carbamazepine (CBZ) is an antiepileptic drug that is used widely for the treatment of epileptic seizures. Neural tube defects (NTDs), growth retardation, and nail hypoplasia are the most common features of teratogenic effects of this drug. The purpose of this study was to examine the effect of vitamin B6 on the developmental toxicity of CBZ on mice. Materials and Methods Sixty B...

[ 4 ] - Effect of Mobile Phone Microwaves on Fetal Period of BALB/c Mice in Histological Characteristics of Hippocampus and Learning Behaviors

Objective(s) The possible risks of radio-frequency electromagnetic fields (EMF) for the living organisms and human body are a growing concern for our society. In this study, we examined the possibility of changes in working memory and hippocampal histological characteristics effects in mice brain following whole body exposure to microwave radiation. Materials and Methods During gestation per...

[ 5 ] - In vitro pro apoptotic effect of crude saponin from Ophiocoma erinaceus against cervical cancer

Ophiocoma erinaceus Muller &Troschel (Ophiocomidae) is part of the extensive group of echinoderm that contains bioactive metabolites. As, the anti cancer potential of brittle star saponin has not been reported against cervical cancer, the present study was conducted to evaluate the anticancer effect of extracted crude saponin. Saponin extraction was conducted using conventional method such as f...

[ 6 ] - Chitin from the mollusc Chiton: Extraction, characterization and chitosan preparation

This study presents the first ever data of extracting chitin from the Chiton shell, which then converted to the soluble chitosan by soaking in the 45% NaOH solution. The obtained chitin and chitosan were characterized by the seven different methods. Antioxidant activity of the extracted chitosan was also evaluated using the two methods. The shell content was divided into calcium carbonate (90.5...

[ 7 ] - Evaluation of the inhibitory synergic effects of the Persian Gulf brittle star extract and taxol on ovarian cancer A2780cp

Paclitaxel is a current standard chemotherapeutic drug for ovarian cancer with several side effects. Recurrences of drug resistant clones have been considered the serious problem in the failure of chemotherapy. Medicinal marine natural products have been intensively proposed as diverse chemotherapeutic agents. Therefore there is an affinity to find efficient modality to overwhelm ovarian cancer...

[ 8 ] - Chitosan extracted from the Persian Gulf chiton shells: Induction of apoptosis in liver cancer cell line

Here for the first time, we investigated the cytotoxic effects of the chitosan extracted from the Persian Gulf Chiton shell (Acanthopleura vaillantii) on liver cancer cell line (HepG2). Chitosan extraction was implemented following this method: chitin was produced by demineralization and deproteinization procedure, and the extracted chitin was converted into soluble chitosan using deacetylation...

[ 9 ] - In vitro pro apoptotic effect of crude saponin from Ophiocoma erinaceus against cervical cancer

Ophiocoma erinaceus Muller &Troschel (Ophiocomidae) is part of the extensive group of echinoderm that contains bioactive metabolites. As, the anti cancer potential of brittle star saponin has not been reported against cervical cancer, the present study was conducted to evaluate the anticancer effect of extracted crude saponin. Saponin extraction was conducted using conventional method such as f...

[ 10 ] - Chitin from the mollusc Chiton: Extraction, characterization and chitosan preparation

This study presents the first ever data of extracting chitin from the Chiton shell, which then converted to the soluble chitosan by soaking in the 45% NaOH solution. The obtained chitin and chitosan were characterized by the seven different methods. Antioxidant activity of the extracted chitosan was also evaluated using the two methods. The shell content was divided into calcium carbonate (90.5...

[ 11 ] - بررسی اثر نانوذره نقره با پوشش عصاره گیاه بومادران بر رگ زایی در مدل پرده کوریوآلانتوئیک جنین جوجه

سابقه و هدف: رگزایی در بیماری هایی زیادی نظیر سرطان دخالت دارد. امروزه استفاده از نانوذرات نقره در صنعت و پزشکی مورد توجه قرار گرفته است. عصاره گیاه بومادران زرد دارای خاصیت های آنتی باکتریال، ضدالتهابی و آنتی اکسیدانی است. در پژوهش حاضر اثر نانوذرات نقره با پوشش عصاره گیاه بومادران زرد بر رگزایی در پرده کوریوآلانتوئیک جنین جوجه بررسی گردیده است. مواد و روش ها: در این پژوهش تجربی 50 عدد ...

[ 12 ] - بررسی اثرات آپوپتوتیک نانوذرات نقره پوشش دار شده با عصاره برگ آویشن شیرازی بر رده سلولی HepG2

سابقه و هدف: نانوذرات نقره (AgNPs) دارای فعالیت ضد باکتری، ضد رگزایی و ضد سرطان می باشند. هدف از این مطالعه بررسی اثرات آپوپتوتیک AgNPs با پوشش عصاره برگ آویشن شیرازی بر سلول های سرطانی کبد، رده HepG2، می باشد. مواد و روش ها: سلول های سرطانی رده HepG2 با غلظت های مختلف AgNPs در زمان های 24 و 48 ساعت تیمار شدند. میزان زیست پذیری سلول ها و غلظت مهاری (IC50) با آزمونMTT محاسبه شد. جهت بر...

[ 13 ] - بررسی اثر نانو ذرات نقره پوشش دار شده با سیلیمارین بر آنژیوژنز در پرده کوریوآلانتوئیک جنین جوجه و ارزیابی فعالیت آنتی اکسیدانی آن

Background: Angiogenesis is a complex, active phenomenon, which plays a significant role in physiologic and pathologic processes. Silymarins are combination of flavonoids that have antioxidant and anti-inflammatory effects. In the current research, the effects of silver nanoparticles coated with silymarin on the angiogenesis of the chorioallantoic membrane of chicken fetal and its antioxidant p...

[ 14 ] - Evaluation of the inhibitory synergic effects of the Persian Gulf brittle star extract and taxol on ovarian cancer A2780cp

Paclitaxel is a current standard chemotherapeutic drug for ovarian cancer with several side effects. Recurrences of drug resistant clones have been considered the serious problem in the failure of chemotherapy. Medicinal marine natural products have been intensively proposed as diverse chemotherapeutic agents. Therefore there is an affinity to find efficient modality to overwhelm ovarian cancer...

[ 15 ] - Chitosan extracted from the Persian Gulf chiton shells: Induction of apoptosis in liver cancer cell line

Here for the first time, we investigated the cytotoxic effects of the chitosan extracted from the Persian Gulf Chiton shell (Acanthopleura vaillantii) on liver cancer cell line (HepG2). Chitosan extraction was implemented following this method: chitin was produced by demineralization and deproteinization procedure, and the extracted chitin was converted into soluble chitosan using deacetylation...

[ 16 ] - Anti-Vasculogenic Activity of a Polysaccharide Derived from Brittle Star via Inhibition of VEGF, Paxillin and MMP-9

Background: Bioactive compounds such as terpenoids, chondroitin sulfate, and polysaccharides with added value can be found in prestine marine creatures. These compounds often do have highly valuable therapeutic applications such as being antioxidant, antitumorogenic, anti-infl ammatory and anti-angiogenic. For the latter, varieties of angiogenesis factors can suppress this issu...

[ 17 ] - Anticancer Properties of Chrysin on Colon Cancer Cells, In vitro and In vivo with Modulation of Caspase-3, -9, Bax and Sall4

Background: The SALL4/Sall4 is constitutively expressed in human and mice. SALL4 mRNA could be used as a marker for the diagnosis of different types of cancers. On the other hand, chrysin has diverse biological properties. Objectives: In the present study, the effect of the chrysin was investigated on the CT26 colon cancer in vitro and in vivo. Furthermore, the expression levels of the stem cel...

[ 18 ] - Sensitization of Resistance Ovarian Cancer Cells to Cisplatin by Biogenic Synthesized Silver Nanoparticles through p53 Activation

Today, drug resistance is one of the major problems in fight against cancer. Therefore, combination of therapeutic strategies was raised to effectively improve disease prognosis. In this regard, silver nanoparticles (AgNPs) are considered significant due to their anticancer properties. This study aimed to return sensitivity to cisplatin to A2780 cisplatin-resistance cell lines in the presence o...

[ 19 ] - The synergistic influence of the sea cucumber (Holothuria arenicola) extract and dacarbazine on murine melanoma cancer cells B16F10

Melanoma has been reported a contemporary malignant cancer. Here, anti-cancer effects of sea cucumber extract (SCE) have been examined on melanoma cells and compared with dacarbazine. MTT assay and morphological analysis were performed to evaluate cytotoxic effects of methanolic SCE Also, several methods were exerted to detect cell dying by SCE and dacarbazine. The MTT assay showed that B16F10 ...

[ 20 ] - Sensitization of Resistance Ovarian Cancer Cells to Cisplatin by Biogenic Synthesized Silver Nanoparticles through p53 Activation

Today, drug resistance is one of the major problems in fight against cancer. Therefore, combination of therapeutic strategies was raised to effectively improve disease prognosis. In this regard, silver nanoparticles (AgNPs) are considered significant due to their anticancer properties. This study aimed to return sensitivity to cisplatin to A2780 cisplatin-resistance cell lines in the presence o...

[ 21 ] - Investigation apoptotic effects of silver nanoparticles coated with Achillea biebersteinii extract on A2780 ovarian cancer cells

In recent years using of silver nanoparticles due to its unique properties was increased. Medical plant, the Achillea biebersteinii is rich in anti-cancers compounds. The aim of this study was to investigate the cytotoxicity effects of AgNPs synthesized using Achillea biebersteinii extract on human ovarian cancer A2780 cells. Cytotoxic effects of AgNPs with MTT test was performed at 48 hours in...

[ 22 ] - Anti-inflammatory properties of saponin fraction from (Spiny brittle star) Ophiocoma erinaceus (Müller and Troschel, 1842)

Marine saponins are naturally glycosides compounds which possess important biomedical properties such as anti-cancer, antioxidant and anti-inflammatory. Nevertheless, no systematic studies on the anti-inflammatory potential of brittle star saponin and its underlying mechanisms have been reported. In this study, Ophiocoma erinaceus extracted saponin was investigated for its in vitro anti-inflamm...

[ 23 ] - The regenerative properties of the extracted polysaccharide from Brittle star (Ophiocoma erinaceus) on cutaneous wound in male Wistar rat

In traditional medicine, extracts of polysaccharide-containing plants are widely employed for the treatment of cutaneus wounds. The brittle star, as one of marine organisms, encompasses bioactive compounds, which confer the healing potency of damaged arms. The aim of the present study is to investigate the effects of extracted polysaccharide from the Persian Gulf brittle star )Ophiocoma erinace...

[ 24 ] - The regenerative properties of the extracted polysaccharide from Brittle star (Ophiocoma erinaceus) on cutaneous wound in male Wistar rat

In traditional medicine, extracts of polysaccharide-containing plants are widely employed for the treatment of cutaneus wounds. The brittle star, as one of marine organisms, encompasses bioactive compounds, which confer the healing potency of damaged arms. The aim of the present study is to investigate the effects of extracted polysaccharide from the Persian Gulf brittle star )Ophiocoma erinace...

[ 25 ] - بررسی اثر اگزوزوم های مشتق شده از سلول های بنیادی مغز استخوان موش کوچک آزمایشگاهی بر سطوح هورمون های استرادیول و تستوسترون مترشحه از سلولهای گرانولوزای تخمدان موش نابالغ نژاد NMRI

امروزه مشکل ناباروری در دنیا به ‌صورت یک نگرانی اجتماعی درآمده است و می‌تواند ضربه روانی شدیدی به زوجین وارد سازد. از مهمترین سلول های تخمدان، سلول های گرانولوزا می باشند که رشد و بلوغ اووسیت ها را تحت تاثیر قرار می دهند. اگزوزوم ها یک کلاس ویژه از وزیکول های خارج سلولی می باشند که می توانند با سیگنال رسانی به سلول ها باعث بروز پاسخ های سلولی شوند. هدف از این پژوهش تجربی بررسی ترشح هورمون های ا...

[ 26 ] - CYTOTOXIC EFFECTS OF ARTEMISIA ABSINTHIUM EXTRACT ON A2780 CELL LINE (OVARIAN CANCER) AND ALTERATION OF APOPTOTIC GENES EXPRESSION LEVELS

Background & Aims: Ovarian cancer is the third most common cancer in women. Artemisia is one of the most commonly used medicinal plants, and Artemisia absinthium is one of the important species of this genus. The aim of this study was to evaluate the effect of cytotoxicity and the ability to induce apoptosis methanolic extract of A. absinthium in A2780 cell line (human ovarian cancer). Materia...