S.A. Pourbakhsh
Mycoplasma Reference Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran
[ 1 ] - Isolation and Identification of Mycoplasma agalactiae by Culture and Polymerase Chain Reaction Methods in the Sheep Herds in Guilan Province, Iran
Contagious agalactia is an infectious syndrome of sheep that is characterized by mastitis with reduction of milk production, arthritis, abortion, and keratoconjunctivitis. The disease is rapidly spread by the contact of the infected animals with the healthy ones. Domestic sheep and goats of both sexes can be infected at an equivalent frequency. Most of the researchers use culture and molecular ...
[ 2 ] - Detection of Mycoplasma agalactiae in Small Ruminants of Southeast Iran
Agalactia is an infectious and contagious disease of small ruminants caused by Mycoplasma agalactiae (M. agalactiae). Although different microorganism strains contribute to this disease, M. agalactiae is known as the most prominent causative agent. Therefore, this study aimed to investigate the rate of M. agalactiae involvement in contagious agalactia in the southeast region of Iran. Sampling ...
[ 3 ] - Isolation and Detection of Mycoplasma agalactiae from Semen Samples of Goats
Contagious agalactia (CA) is a highly infectious disease of goats and sheep, and is a form of Mycoplasmosis,which is usually enzootic. Since Mycoplasma agalactiae (M. agalactiae) is the main cause of this disease ingoats, the aim of this study was to isolate and detect M. agalactiae from semen of goat bucks. Thirty-nine semensamples were collected from goat bulks, and all samples were cultured ...
[ 4 ] - Molecular characterization of Mycoplasma synoviae isolates from commercial chickens in Iran
Detection of Mycoplasma synoviae (MS) by culture and polymerase chain reaction (PCR) has been reported from commercial chicken farms in different provinces of Iran. In some reports the phylogenetic analysis of MS isolates based on 16S rRNA and variable lipoprotein hemagglutinin (vlhA) genes have been carried out. The PCR product containing partial 16S rRNA genes of Iranain isolates was sequence...
[ 5 ] - The vlhA gene sequencing of Iranian Mycoplasma synoviae isolates
Mycoplasma synoviae expressed variable lipoprotein haemagglutinin (VlhA) is believed to play a major role in pathogenesis of the disease by mediating adherence and immune evasion. The aim of this study was sequencing Iranian M. synoviae isolates for the detection of nucleotide variation in the M. synoviae vlhA gene. Using oligonucleotide primers complementary to the single-copy conserved 5´ end...
[ 6 ] - Isolation and identification of Mycoplasma agalactiae by culture and polymerase chain reaction (PCR) from sheep of Qom province, Iran
Contagious agalactia (C.A) is an infectious syndrome of sheep that is characterized by mastitis and subsequent failure of milk production, arthritis, abortion and keratoconjunctivitis. Mycoplasma agalactiae (M. agalactiae) is the main cause of the disease in sheep. The aim of this study was isolation and identification of M. agalactiae with culture and polymerase chain reaction (PCR) assay from...
[ 7 ] - Application of culture and polymerase chain reaction (PCR) methods for isolation and identification of Mycoplasma synoviae on broiler chicken farms
Mycoplasma synoviae (M. synoviae) is a major worldwide poultry pathogen that causes serious economic losses in the poultry industry. This study was designed to detect M. synoviae through culture isolation and polymerase chain reaction (PCR) assay to demonstrated the involvement of M. synoviae infection in trachea and the lung/air sac samples taken from commercial broiler chicken farms in 3 m...
[ 8 ] - Prevalence and molecular characterization of verotoxin-producing Escherichia coli O157:H7 in cattle and sheep in Shiraz-Iran
Shiga toxin producing Escherichia coli have been associated with HUS, HC and TTP in human. We found recto-anal mucosal sample in sheep as well in cattle is the main site for E. coli O157 localization. 1246 E. coli isolates from 872 both healthy and diarrheic animals were analyzed, by screening for the presence of Shiga toxin-producing (VT 1 and VT 2) and intimin (eae) genes used Multiplex PCR. ...
[ 9 ] - Detection of Mycoplasma synoviae infection in broiler breeder farms of Tehran province using PCR and culture methods
Mycoplasma synoviae (MS) is an important avian pathogen that can cause both respiratory disease and joint inflammation synovitis in poultry, inducing economic losses to the Iranian chicken industry especially breeder farms. The aim of this study was to use the MS specific PCR and culture methods in order to detect of M. synoviae from breeder farms where located in Tehran province. A total of 47...
[ 10 ] - Using PCR and culture methods for Mycoplasma testing
Mycoplasma contaminants can be considered important not only for their roles as pathogens but also they may indicate that insufficient care has been taken during vaccine manufacture or quality control. The purpose of this study was to test the poliomyelitis vaccines for Mycoplasma by culture and polymerase chain reaction (PCR) methods. During 2008 to 2009, a total of 47 lots of oral poliomyelit...
[ 11 ] - Capripoxvirus identification by PCR based on P32 gene
Sheeppox virus (SPV) and goatpox virus (GPV) belong to the capripoxvirus genus of Poxviridae family. Sheeppox and goatpox along with contagious ecthyma (CE) are endemic diseases in Iran. Capripox laboratory conformation based on virological and serological techniques are time consuming, laborious and most of them of low specificity, because of close antigenic relationship between capripoxviru...
[ 12 ] - Ninetythree B type, the Predominant Circulating Type of Avian Infectious Bronchitis Viruses 1999 - 2004 in Iran: a retrospective study
In order to identify predominant circulating types of infectious bronchitis(IB) virus in the period of 1999-2004 in Iran, samples(trachea, lung, kidney) from 150 various flocks showing respiratory signs suspected to be related to IB infection were inoculated to SPF eggs. Then allantoic fluids studied by RT-PCR and Nested PCR using group and type specific primers to probing Massachusset, 793/B...
[ 13 ] - S7 gene Characterization of Bluetongue Viruses in Iran
Bluetongue is an infectious disease that primarily affects sheep. But due to serious socioeconomic consequence of it outbreaks on the international trade it has been included in the OIE notifiable diseases (list A). During 2007-8, total number of 130 blood samples gathered from suspected sheep to bluetongue disease in seropositive region including Khuzestan, Kurdistan, Fars, Ilam and Qum prov...
[ 14 ] - Cloning of fusion (F) protein gene of peste des petits ruminants virus (PPRV) in secretory Pichia pastoris vector
With advent and development of DNA recombinant technology and advantages of p. pastoris expression system, fusion (F) protein of PPRV expression, because of effective immunodominant role could be an appropriate candidate for production of recombinant vaccine against PPR disease. In this study, F gene of PPRV Nigeria 75/1 strain (1637 bp) was amplified using RT-PCR and purified. It was then cl...
[ 15 ] - Application of polymerase chain reaction in detection of egg drop syndrome (EDS) virus
Egg drop syndrome (EDS) is caused by a hemagglutinating adenovirus which has become a major cause of lost egg production and sever economic losses in breeder and layer chicken flocks throughout the world. A PCR assay was optimized for detection of EDS virus in inoculated allantoic fluids (AFs) of duck and chicken emberyonated eggs. Two strains of EDS viruses were propagated in allantoic cavit...
[ 16 ] - Evaluation of maternal antibody levels for establishing the vaccination program against Newcastle disease in ostrich chicks
Newcastle disease virus (NDV) is known as one of the most important endemic viral pathogen for various avian species such as ostrich, in Iran. Therefore, establishing a routine vaccination program against ND in ostrich flocks would be useful in order to reduce the danger of this infection. Newcastle disease occurs among the ostriches and leads to high rate of mortality while most of the losse...
[ 17 ] - Study on Mycoplasmal pneumonia at the Ziaran abattoir
I n a survey of pneumonia due to Mycoplasma 282 out of 12168 ovine and caprine lung condemnation were collected (2.32%). Mycoplasma spp. has been isolated from pneumonic cases in 4 sheep and 2 goats. PCR studies were confirmed the genus of Mycoplasma although attempting for identification of strains M. mycoides, M. capricolum/caprine pleuropneumonia and M. arginin were in failure. The lesions...
[ 18 ] - Neuraminidase gene sequence analysis of avian influenza H9N2 viruses isolated from Iran
Influenza A viruses possesses two virion surface glycoproteins including haemagglutinin (HA) and neuraminidase (NA). The NA plays an important role in viral replication and promotes virus release from infected cells and facilitates virus spread throughout the body. To find out any genomic changes that might be occurred on NA gene of avian influenza circulating viruses, we have genetically analy...
[ 19 ] - Application of PCR on detection of aflatoxinogenic fungi
Aflatoxins are carcinogenic metabolites produced by several strains of Aspergillus flavus group in food and feed. Cluster genes in aflatoxin biosynthesis pathway contain structural, regular and unassigned genes , nor-1 , ver-1 , and omt-1 are structural genes that coding for key enzymes and aflR is a regulatory gene that plays a key role in the production of aflatoxin and is affecting on the s...
[ 20 ] - Plaque formation of LaSota pathogenic strain of Newcastle disease virus adapted in chick embryo fibroblast cells
In order to adapt LaSota strain of Newcastle disease virus (NDV) on chick embryo fibroblast (CEF) cells, 0.1 ml from LaSota vaccine produced in Razi vaccine and serum research institute inoculated to the CEF cells and was passaged five times in the CEF grown in Eagle’s minimum essential medium (MEM). First to third passages were blind but in fourth passage cytopathic effect of virus was observe...
[ 21 ] - Experimental histopathologic study of the lesions induced by serotype 793/B (4/91) infectious bronchitis virus
The study was conducted to determine the type, frequency and severity of gross, histopathologic changes, effect of route of infection and also, tissue tropism of serotype 793/B (4/91) infectious bronchitis virus. 20 day-old specific pathogen free chicks were inoculated intra-tracheally and intra-orally with 793/B serotype and lesions studied histopathologically at interval up to 10 days post-in...
[ 22 ] - Isolation and pathogenicity identification of avian paramyxovirus serotype 1 (Newcastle disease) virus from a Japanese quail flock in Iran
Newcastle disease virus (NDV) was isolated and identified for the first time from a flock of Japanese quail suspected to ND in Iran. Birds of the flock aged variously, and some of them died from the disease. Loss of appetite, weakness and decrease in egg production, diarrhea and nervous symptoms were the main clinical signs in the birds. Hemorrhagic lesions of the intestinal tracts and proventr...
[ 23 ] - Expression of the VP2 gene of classical D78 infectious bursal disease virus in the methylotrophic yeast Pichia pastoris as a secretory protein
Infectious bursal disease virus (IBDV) is the causative agent of Gumboro disease, an infectious disease of global economic importance in poultry. The expression of heterologous proteins in P.pastoris is fast, simple and inexpensive. In this study, VP2 encoding gene of classical D78 IBDV was amplified using reverse transcription (RT) polymerase chain reaction (PCR) and cloned into pPICZαA vector...
[ 24 ] - Random amplified polymorphic DNA (RAPD) fingerprinting of Mycoplasma gallisepticum isolates from chickens
Ten field isolates of Mycoplasma gallisepticum (MG) from different geographical areas of Iran were analyzed by random amplification of polymorphic DNA (RAPD). RAPD analysis produced reproducible banding patterns on the basis of which various distinct amplification patterns could be detected. MG isolates compared with reference strains (S6 and Mg SS) and vaccine strain (ts-11) and demonstrated...
[ 25 ] - PCR detection of Escherichia coli O157:H7 directed from slaughtered cattle in Shiraz, Iran
Escheric hia coli O157:H7 lives in the intestines of healthy cattle, and can contaminate meat during slaughtering pr actices . Detection of the low infectious dosage of bacterium requires a sensitive method. We developed polymerase chain reaction (PCR) assays to detect the gene Stx2 irrespective of the bacterial serotype. In this study, the detection limit of the PCR protocol in detecting Stx...
[ 26 ] - Detection of Infectious Bronchitis Virus in Allantioc Fluid by Rapid Hemagglutination Test.
To detect the presence of infectious bronchitis virus (IBV) in infected allantoic fluid (AF) of SPF embryonated eggs rapid hemagglutination (HA) activity after treatment with neuraminidase enzyme was used. Twenty IBV suspected materials were inoculated in SPF embryonated eggs via chorioallantoic cavity. Harvested AFs were treated with neuraminidase enzyme and the presence of IBV was detected by...
[ 27 ] - Standardization of an Enzyme-Linked Immunosorbent Assay for Detection of Infectious Bronchitis Virus Antibody.
An indirect enzyme–linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectious bronchitis virus (IBV). Antigen was prepared from whole-purified IBV Massachusetts serotype (BR 801 strain). Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed ELISA was determined 0.1μg/ml, 1:3000 and 1:100, respe...
[ 28 ] - Preparation of an Inactivated Egg Drop Syndrome Antigen for Using in Hemagglutination Inhibition Test.
To prepare hemagglutination inhibition (HI) antigen the egg drop syndrome virus (EDSV) was propagated in 10-day-old embryonated duck eggs. The virus antigen was inactivated with two methods including heating (65°C) and adding 0.5% formaldehyde by considering low destroying effect on hemagglutination (HA) and HI titers. The EDSV HI titers of 310 sera and 100 yolks obtained from 23 chicken farms ...
[ 29 ] - Pathogenicity Study and Restriction Enzyme Profile of a Recently Isolated Infectious Bursal Disease Virus in Iran
The pathogenicity of a recent isolate of Infectious bursal disease virus (IBDV), IR499, isolated from a nonvaccinated flock with 17.5 % mortality rate in susceptible SPF chickens, chickens embryos and broilers was discussed. The molecular characterization of the virus based on the restriction fragment length polymorphism (RFLP) pattern was also investigated. The mortality rates were 85% and 22%...
[ 31 ] - Antibiotic Sensitivity of Ornithobacterium rhinotracheale Isolates Associated with Respiratory Diseases.
187 commercial checken flocks affected with respiratory diseases were examined for Ornithobacterium rhinotracheale isolation. The bacterium was isolated from 105 (56.2%) poultry flocks. Drug sensitivity test using standard disk diffusion technique was performed with 19 antibiotics. All the isolates were susceptible to tiamulin and most of them were susceptible to chloramphenicol and linco-spect...
[ 37 ] - فیلوژنی سویههای واکسن مایکوپلاسما آگالاکتیه موجود در ایران با استفاده از روش (Multi Locus Sequence typing (MLST
مایکوپلاسما آگالاکتیه یکی از عوامل اصلی ایجاد آگالاکسی در نشخوارکنندگان کوچک میباشد. ایران یکی از کانونهای اصلی اپیدمیهای سالانه این بیماری در جهان شناخته میشود. واکسن کشته آگالاکسی با استفاده از سه سویه بومی شیراز، طالقان و لرستان در موسسه رازی تولید میشود. به منظور شناسایی ویژگیهای ژنتیکی این سویهها سیستم تایپینگMulti Locus Sequence typing (MLST)i با تمرکز بر روی 5 ژن dnaA ،gltX ،gyrB ...
[ 38 ] - شناسایی مایکوپلاسما بویس در گاوهای مبتلا به ورم پستان بالینی با استفاده از کشت و روش واکنش زنجیرهای پلیمراز بر اساس ژنهای 16SrRNA و uvrC
مایکوپلاسما بویس از عوامل اصلی ایجاد پنومونی، ورم پستان و آرتریت مایکوپلاسمائی در گاو میباشد. هدف از این مطالعه، شناسایی مایکوپلاسما بویس در گاوهای مبتلا به ورم پستان بالینی با استفاده از کشت و روش زنجیرهای پلیمراز بود. این مطالعه روی تعداد 328 نمونه شیر گاو مبتلا به ورم پستان بالینی که در طول یک سال به آزمایشگاه مرجع مایکوپلاسمای مؤسسه تحقیقات واکسن...
[ 39 ] - همسانی ژنتیکی سویه های واکسن مایکوپلاسما آگالاکتیه جدا شده از طالقان، لرستان و شیراز در ایران
سابقه و هدف: ایران یکی از اصلی ترین کانون های فعالیت مایکوپلاسما آگالاکتیه در دام های نشخوارکننده در جهان شناخته است. سه سویه بومی طالقان، لرستان و شیراز برای تولید تنها نمونه واکسن کشته علیه آگالاکسی در ایران مورد استفاده قرار می گیرند. این مطالعه با هدف آگاهی از ارتباط ژنتیکی میان این سویه ها انجام شد. مواد و روش ها: از هر سه سوی...
Co-Authors
A. Nouri 1