محمد حسین آبنوسی
استادیار، دانشکده علوم، دانشگاه اراک
[ 1 ] - تأثیر فلز کادمیم بر توانائی زیستی و فعالیت برخی آنزیم‌های پاداکسندگی در پینة گیاه پریوش (Catharanthus roseus)
پریوش گیاهی زینتی و داروئی است که ازنظر اقتصادی نیز اهمیت دارد. از پینة (کالوس) گیاه پریوش دروایة (سوسپانسیون) یاخته‌ای تهیه و پس از تیمار با دزهای مختلف نیترات کادمیم به مدت 1، 3، 6 روز توانائی زیست توسط تریپان بلو و MTT بررسی شد. سوسپانسیون یاخته‌ای برای بررسی ریخت‌شناسی (مورفولوژی) و بافت پینه برای بررسی فعالیت آنزیم‌های کاتالاز، گایاکول پراکسیداز و سوپراکسید دیسموتاز و پراکسیداسیون لیپیدی پ...
[ 2 ] - Cadmium chloride treatment of rats significantly impairs membrane integrity of mesenchymal stem cells via electrolyte imbalance and lipid peroxidation, a possible explanation of Cd related osteoporosis
Objective(s): Bone marrow mesenchymal stem cells (MSCs) play an important role in bone health. Cadmium causes osteoporosis, but the exact mechanisms of its effect on MSCs are not known. Materials and Methods: Rats were treated with cadmium chloride (40 mg/l) in drinking water for six weeks, and then the biochemical and morphological studies on MSCs were carried out as a cellular backup for oste...
[ 3 ] - Biochemical and morphological changes in bone marrow mesenchymal stem cells induced by treatment of rats with p-Nonylphenol
Objective(s):In previous investigations, we have shown para-nonylphenol (p-NP) caused significant reduction of proliferation and differentiation of rat bone marrow mesenchymal stem cells (MSCs) in vitro. In this study, we first treat the rats with p-NP, then carried out the biochemical and morphological studies on MSCs. Materials and Methods: Proliferation property of cells was evaluated with t...
[ 4 ] - Para-Nonylphenol Impairs Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells by Influencing the Osteoblasts Mineralization
Objective(s)Para-Nonylphenol (p-NP) is used in many industries and our previous study showed that p-NP causes a reduction in rats bone marrow mesenchymal stem cells (MSCs) viability. The aim of this study was to investigate the effect of p-NP on osteogenic differentiation of MSCs.Materials and MethodsMSCs were isolated and expanded to 3rd passage, then cultured in DMEM supplemented with osteoge...
[ 5 ] - Induction of Apoptosis in the Rat Bone Marrow Mesenchymal Stem Cells Following Sodium Arsenite Treatment with the Dose Lesser than that Used for Treatment of Malignant Patient
Objective(s) Arsenic compounds are potent human carcinogen and produce a variety of stress responses in mammalian cells. Recently sodium arsenite has been recommended to be used as anti malignancy drug by American food and drug administration (FDA). In this study, we aimed to determine the apoptosis inducing effect of sodium arsenite on rat bone marrow mesenchymal stem cells exposed in vitro. ...
[ 6 ] - Myo-inositol at High Concentration Reduced Viability and Proliferation of Rat Bone Marrow Mesenchymal Stem Cells via Electrolyte Imbalance and Elevation of Aerobic Metabolism
Myo-inositol (MI) which is produced at low concentration is an essential substance for animal’s natural growth. This study was performed to investigate the effects of MI on viability, proliferation and some biochemical factors of rat bone marrow mesenchymal stem cells (BMSCs). To investigate the cell viability using trypan blue assay, BMSCs after third passage were treated with different concen...
[ 7 ] - Biochemical and Cellular Response of Catharanthus roseus Callus Cells to Cadmium Toxicity
Catharanthus roseus is a medicinal and ornamental plant with growing attention toward its economical value. Cell suspension from C. roseus were treated with 0 to 60mM of cadmium nitrate for 1, 3 and 6 days, then cell viability was determined using trypan blue and MTT assay. Cell morphology was investigated using 0, 10, 30 and 50 mM of cadmium nitrate as selected dose for 3 days. Callus of C.ros...
[ 8 ] - Cadmium treatment of rats caused impairment of osteogenic potential of bone marrow mesenchymal stem cells: a possible mechanism of cadmium related osteoporosis
Background: The mechanism of cadmium induced osteoporosis is not well understood, so in this study, we examined the toxicity of bone marrow mesenchymal stem cell (MSCs) following treatment of rats with CdCl2 in drinking water, to revile the effect of this chemical on differentiation potential of MSCs. Methods: At the end of third passage, MSCs were grown in the osteogenic medium for 21 days....
[ 9 ] - Para-nonylphenol Toxicity Induces Oxidative Stress and Arrests the Cell Cycle in Mesenchymal Stem Cells of Bone Marrow
Background: The mechanism of para-nonylphenol (PNP) reducing the proliferation and differentiation of bone marrow mesenchymal stem cells (MSCs) is not known. The present study was designed to investigate the mechanism. Methods: MSCs were extracted under sterile condition from Wistar rat and cultured in DMEM, containing 15 % FBS and penicillin/streptomycin until the 3rd passage, then cells we...
[ 10 ] - Di-ethanolamine Might Cause Bone-related Complications Due to the Reduction of Osteogenic Differentiation and Induction of Oxidative Stress
Di-ethanolamine (DEA) is a well-known environmental pollutant used in manufacturing soap, detergent, body lotion, and other sanitary products. DEA has been reported to cause cytotoxicity in different tissue and cell, but no study was found to explain the toxic effect of DEA on rat bone marrow mesenchymal stem cells (BMSCs) differentiation. Thus in the present study, the differentiation property...
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