Keramati, Malihe
Assistant Professor, PhD of Pharmaceutical Biotechnology, Nano-biotechnology Department, Pasteur Institute of Iran (PII), Tehran, Iran
[ 1 ] - Evaluation and comparison of affinity chromatography and precipitation– based methods on purification of recombinant streptokinase
Background: Increase of protein purity is a serious challenge in the production of recombinant therapeutic proteins. For this purpose, several strategies have been employed to purify the target protein, among which the affinity chromatography-based purification methods and tagged proteins such as Ni-NTA are common and but costly. Therefore column-free purification techniques, such as using elas...
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