Singhal S

[ 1 ] - P-34: Effect of Including Growth Factors andAntioxidants in Maturation Medium Used forIn vitro Culture of Buffalo Oocytes RecoveredIn vivo

Background: This study examined the effect of including one of two growth factors (100 ng/ml IGF-1 or 20 ng/ ml EGF) in combination with one of two antioxidants (50 μM cysteamine or 50 μM beta-mercaptoethanol) in invitro maturation of buffalo oocytes, followed by fertilization and subsequent development of embryos. Materials and Methods: The oocytes were recovered by in vivo ovum pick-up techni...