Asgari V
[ 1 ] - P-63: Comparison of The Efficacy of Two Culture Media (Handmade vs. Commercial) on In Vitro Development of Vitrified and Non-Vitrified Mouse Embryos
Background: While assisted reproductive techniques (ARTs) are considered to be safe, many concerns exist about the safety of these techniques. Two important and indispensable parts of these techniques are in vitro culture and vitrification. While much progress has been made in developing culture conditions, it remains suboptimal. In order to investigate the effect of culture medium on in vitro ...
[ 2 ] - P-83: Cloned Sheep Blastocysts Derived from Oocytes Enucleated Manually Using A Pulled Pasteur Pipette
Background: The potential applications of a simplified method of somatic cell nuclear transfer (SCNT) that is improved in both efficiency and throughput is considerable. Technically, a major step of SCNT is to produce large pools of enucleated oocytes (cytoplasts) efficiently, a process that requires considerable micromanipulation skill and expensive equipment. Here, we have devel oped an efficient...
[ 3 ] - P-86: Determining The Most Oocyte Challenging Step during Vitrification-Warming in Sheep
Background: Oocyte cryopreservation is of utmost importance in the current repertories of assisted reproductive technology (ART). Despite this, oocyte vitrification is yet a problematic issue with limited successful pregnancies has been arisen after transfer of embryos derived from vitrified-warmed oocytes. In this sense, great attention has been paid to investigate possible mechanisms underlyi...
[ 4 ] - P-197: Aberrant Gene Expression Profile in Blastocyst Embryos As Consequences of Assisted Reproductive Technologies
Background: Currently, ARTs are of indispensable importance in treatment of human infertility. While ARTs are considered to be safe, some studies suggest that ARTs manipulations may perturb the epigenetic information and subsequently the health of the offspring. To examine these events further, this study was conducted to assess the effect of superovulation, in vitro culture and vitrification o...
[ 5 ] - P-92: Specific Activation Requirements of In Vitro Matured Sheep Oocytes following Vitrification-Warming
Background: Oocyte vitrification and assisted oocyte activation have increasingly important implications in assisted reproductive technology. However, an important area of concern with matured oocyte cryobiology is that elements of oocytes intimately involved in metaphase-II arrest may be modified by cryopreservation. In a comparison between different cellular characteristics of unvitrified, vi...
[ 6 ] - P-94: Mouse Embryo Vitrification Cannot Effect on Global DNA Methylation in Preimplantation Stage
Background: Embryo vitrification was effectively used for assisted reproductive techniques. Despite the undeniable benefits of vitrification, cooling and warming stress, and cytotoxicity of cryoprotectant may affect the DNA methylation that have an important role in gene activation and silencing. In the present study effects of 2-cell embryo vitrification on DNA methylation in hatched blastocys...
[ 7 ] - P-98: Effect of Mouse Embryo Vitrification on Histone Modifications
Background: Vitrification has been usually used as an assisted reproductive technology in animals and humans. This method needs high concentrations of cryoprotectants that can be toxic with high cooling degrees. Then, vitrification could be change histone modifications such as methylation and acetylation can performance as regulatory controls of gene transcription. So, the purpose of the presen...
[ 8 ] - P-112: Potential Applications of Sheep Oocytes As Affected by Vitrification and In Vitro Aging
Background: The present study was carried out to investigate how the interactions between aging, vitrification, and post-warming interval affect the credibility of sheep MII-oocytes for in vitro fertilization (IVF), intracytoplasmic injection (ICSI), and parthenogenetic activation (PA). Materials and Methods: The vitrified-warmed oocytes, either immediately (immediate group: IG) or two hours po...
[ 9 ] - P-118: Effect of Vitrification on Developmental Competence of Parthenogenetic Activation in In Vitro Matured Ovine Oocytes
Background: Cryopreservation of in vitro matured oocytes is a useful technique because the oocytes can be used for some assisted reproductive technologies. On the other hand, the cryopreservation of oocytes is an open problem as a result of their structural sensitivity to the freezing process. The purpose of this study was to evaluate the effect of vitrification on in vitro development of vitri...
[ 10 ] - P-22: Time Dependent Effect of Post Warming Interval on Microtubule Organization, Meiotic Status, and Parthenogenetic Activation of Vitrified In vitro Matured Sheep Oocytes
Background: It is a common practice to rest vitrified-warmed matured oocytes for 1-3 hours, as a treatment to recover spindle and cytoskeleton, before commencing a further treatment. Vitrified-warmed matured oocytes, however, are very sensitive and may resume meiosis spontaneously during this recommended rest time. Therefore, the aim of this study was to assess spindle and chromosome status as ...
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