Sharafi M

[ 1 ] - O-10: Sperm Mediated Gene Transfer Using Adjuvant Preserving Fertility for Production of Transgenic Chicken Expressing

Background: Low uptake of exogenous DNA by sperm and reduced number of fertilized oocyte by transfected sperm are the major obstacles for progression of sperm mediated gene transfer. Therefore, the modification of sperm mediated gene transfer procedure needs to be required. The purpose of this study was to evaluate the efficiency of FuGene 6 compare to lipofection in transfection medium for int...

[ 2 ] - P-69: Expression of Leptin Receptor mRNA in Ovine Corpus Luteum

Background: Many hormones are involved in the regulation of reproduction. Leptin hormone which is mainly secreted by adipose tissue plays an important role in energy homeostasis and reproduction. It seems that leptin is an important linkage between body metabolism and reproductive system. Moreover, it has been shown that leptin and leptin receptor express in reproductive organs of some species....

[ 3 ] - P-146: Fertility and Flow Cytometric Evaluations of Frozen-Thawed Rooster Semen in Cryopreservation Medium Containing Low Density Lipoprotein

Background: Frozen-thawed rooster semen is not reliable for use in artificial insemination in commercial stocks. Low density lipoprotein (LDL) has been assessed for effectiveness as a cryoprotectant in the extender to improve the quality of frozen-thawed rooster semen. Although LDL has been evaluated in a few studies in other species for semen cryopreservation, to date, no study has been conduc...

[ 4 ] - P-137: Improvement in Post-Thawed Ram Sperm Quality by Adding Cysteine in Soybean Lecithin Based Extender

Background: Ram sperm have a higher polyunsaturated fatty acids and a lower cholesterol/phospholipid ratio than other species which renders the sperm much more vulnerable to oxidative damage caused by reactive oxygen species (ROS). Oxidative stress can affects the integrity of cells at both the structural and sub structural levels. Therefore, a suitable antioxidant may prevent cryo-injuries and...

[ 5 ] - O-17: Survey the Psychological Disorder ofInfertility in Infertile Couples (Couples whoUndergoing for ART Protocol)

Background: Survey the Psychological disorder of infertility in infertile couples (couples who undergoing for ART protocol): Parenthood is one of the major transitions in adult life for both men and women, which is associated with major emotional sequels. This study was performed to determine the psychological impact of infertility in infertile female (IF) and their partners- normal healthy mal...