Nasr Esfahani MH
[ 1 ] - O-3: Drug Repositioning by Merging Gene Expression Data Analysis and Cheminformatics Target Prediction Approaches
The transcriptional responses of drug treatments combined with a protein target prediction algorithm was utilised to associate compounds to biological genomic space. This enabled us to predict efficacy of compounds in cMap and LINCS against 181 databases of diseases extracted from GEO. 18/30 of top drugs predicted for leukemia (e.g. Leflunomide and Etoposide) and breast cancer (e.g. Tamoxifen a...
[ 2 ] - P-13: Comparison of Sperm Quality, Oxidative Stress, DNA Fragmentation, Protamine Deficiency, and DNA Methylation in Varicocele and Fertile Individuals
s:1993:"Background: There are many approaches that gene expression is controlled in eukaryotes. DNA methylation is one of several epigenetic mechanisms that cells use to control gene expression and lock genes in the "off" position. In addition, sperm DNA damage can correlate with DNA methylation defect. There is evidence that sperm of infertile men contain more DNA damage than fertile men and t...
[ 3 ] - P-59: Effect of Microsurgical Varicocelectomy on Sperm Chromatin Packaging,Miscarriage and Pregnancy Rate in Infertile Men with Varicocele
Background: Although the pathophysiology of the testicular damage associated with varicocele remains unclear, but varicocelectomy is considered a suitable technique for the treatment of individuals with clinical varicocele. The current study was designed to assessed semen parameters, chromatin packaging, and pregnancy outcome before and after varicocelectomy. Materials and Methods: Semen sample...
[ 4 ] - P-74: Effect of Fndc5 Overexpression onCardiac Differentiation Rate of mESCs
Background: Fibronectin type III domain-containing 5 proteins (Fndc5), an exercise hormone, formerly known as peroxisomal protein that was cloned in 2002. Transcript profile analysis of Fndc5 revealed high degree of expression in heart, skeletal muscle and brain. Our recent studies indicated a significant increase (approximately 10 folds) in mRNA level of Fndc5 when mouse embryonic stem cells w...
[ 5 ] - P-118: Stepwise Morphometric Changesduring In Vitro Maturation of Goat Oocytein Relation with Nuclear Remodeling and Developmental Competencee
Background: Successful in vitro maturation (IVM) of oocytes is an important step for production of livestock through assisted reproductive techniques including somatic cell nuclear transfer (SCNT). Obtaining an optimal duration for maturation of oocyte is essential, since over exposure to maturation medium results in oocyte aging, while under exposure leads to production of immature incompetent...
[ 6 ] - P-127: The Effect of Supplementation of In Vitro Culture Medium with FGF2 on Ovine Embryo Development
Background: Fibroblast growth factors are a great family of growth factors that exert variety of functions in vertebrates including cell growth, survival, differentiation and migration. FGFRs are transmembrane tyrosine kinase receptors transmitting FGF signals. Four types of FGFRs (1/2/3/4) exist in vertebrates. FGF2 is one of the FGFs detected in bovine and ovine uterus flushes, supposing that...
[ 7 ] - P-131: Effects of Heat Shock during Early Stage of Oocyte Maturation on Meiotic Progression and Subsequent Embryonic Development and Gene Expressionin Ovine
Background: Heat shock may affect different aspects of oocyte maturation and its subsequent development to the blastocyst stage. A series of in vitro experiments was performed to determine whether physiologically heat shock (41°C) disrupts the progression of the ovine oocytes through meiosis, activation and blastocyst formation. Materials and Methods: The cumulus-oocyte complexes (COCs) were as...
[ 8 ] - P-190: Expression Changes of GDF- 9 and BMP-15 in The Oocytes of PCOS Patients Undergoing Treatment with N-acetylcysteine
Background: Polycystic ovary syndrome (PCOS) is a frustrating experience for women, considered as a complex challenge for researchers and clinicians. Despite the enormous effort to define the cause of PCOS, the etiology and pathogenesis still remain unclear. Oocyte quality is reflected in the oocyte’s intrinsic developmental potential. Studies have shown that the follicular development is disru...
[ 9 ] - O-26: Association of Follicular Fluid Endocrine Changes with Oocyte Morphology and Quality in PCOS Patients Undergoing Metformin and N-Acetylcysteine Treatment
Background: Polycystic ovary syndrome (PCOS) is a common cause of infertility often responsive to treatment with oral ovulation induction agents. PCOS patients are typically characterized by producing an increased number of oocytes that they are often of poor quality, leading to lower fertilization, cleavage and implantation rates. Recently data involve functional associations between endocrine...
[ 10 ] - P-47: Comparison of Sperm Acrosin Activity and DNA Fragmentation between Varicocele and Infertile Individuals
Background: Introduction assessment of sperm functional tests along with routine semen analysis may provide further information in the etiology of varicocele in different subpopulations. Therefore, in this study, in addition to assessment of sperm chromatin integrity, we aimed to assess the total acrosin activity which has proven to have major role during in vitro and natural fertilization in v...
[ 11 ] - P-58: Which Novel Sperm Selection is Suitable for ICSI? MACS-DGC or DGC-MACS
Background: Super-paramagnetic annexin-V conjugated micro-beads are able to eliminate sperm with phosphatidylserine externalization (PSE). PSE is considered as apoptotic sperm. PES positive sperm can be removed from sperm preparations sample to enhance sperm quality for assisted medical procreation. Therefore, the efficiency of this technique over normal sperm processing procedure such as densi...
[ 12 ] - P-77: HOST Patterns and Sperm Quality
Background: Following the expansion of human infertility, different procedures have been utilized for separation of normal sperm during ICSI (Intra cytoplasmic sperm injection). Hypo-osmotic swelling test (HOST) is one of the tests which have been introduced for assessment of integrity of sperm membrane when confronted with hypo osmotic condition. This test may also reflect intracellular organe...
[ 13 ] - P-78: Supplementation of Sperm Cryopreservation Media with Cell Permeable Superoxide Dismutase Mimetic Agent (MnTE) Improves Goat Blastocyst Formation
Background: ROS production is a well-established phenomenon during sperm cryopreservation. Therefore, supplementation of freezing media with antioxidant is an inevitable event. The aim of this study was to assess whether a cell permeable superoxide dismutase agent such as MnTE, can further improve the quality of frozen/ thawed semen sample using a commercially optimized sperm cryopreservation m...
[ 14 ] - P-81: Distinct Epigenetic Status of Fully Grown and Growing Oocytes Selected by BCB Staining
Background: Since the sheep ‘Dolly’ was successfully cloned by somatic cell nuclear transfer (SCNT), the inefficiency of SCNT cloning and abnormality of cloned animals have been a focus of considerable controversy. Oocyte quality has been shown to contribute to poor somatic cloning and cell reprogramming efficiency with many factors affecting oocyte quality and developmental potential. It has b...
[ 15 ] - P-82: Effect of SCNT Steps on Relative mRNA Abundances of Sheep Oocytes
Background: The oocyte is a unique cell committed to reprogram fertilizing sperm and to support early stages of embryonic development until the species-specific stage of zygote genome activation that occurs around the second to third cell cycle in sheep embryos. In this sense, considering the huge list of oocyte transcripts, we selected some candidates genes based on their roles of regulating di...
[ 16 ] - P-83: Cloned Sheep Blastocysts Derived from Oocytes Enucleated Manually Using A Pulled Pasteur Pipette
Background: The potential applications of a simplified method of somatic cell nuclear transfer (SCNT) that is improved in both efficiency and throughput is considerable. Technically, a major step of SCNT is to produce large pools of enucleated oocytes (cytoplasts) efficiently, a process that requires considerable micromanipulation skill and expensive equipment. Here, we have devel oped an efficient...
[ 17 ] - P-86: Determining The Most Oocyte Challenging Step during Vitrification-Warming in Sheep
Background: Oocyte cryopreservation is of utmost importance in the current repertories of assisted reproductive technology (ART). Despite this, oocyte vitrification is yet a problematic issue with limited successful pregnancies has been arisen after transfer of embryos derived from vitrified-warmed oocytes. In this sense, great attention has been paid to investigate possible mechanisms underlyi...
[ 18 ] - P-97: Optimized Combined Electrical-Chemical Parthenogenetic Activation for Zona Free Sheep Oocyte
Background: Artificial oocyte activation (AOA) has important implications in assisted reproductive technique (ART) with particular importance in somatic cell nuclear transfer (SCNT). Parthenogenesis activation using zona- free oocytes offers an alternative model that could be applied to develop protocols for the activation of reconstructed embryos for cloning. Materials and Methods: To establis...
[ 19 ] - P-108: Effect of Phosphodiesterase Type 3 Inhibitor on Nuclear Maturation and In Vitro Development of Ovine Oocytes
Background: Present study aims to investigate if PMC of ovine oocytes in the presence of cilostamide a phosphodiesterase type 3 (PDE3) inhibitor can improve the shortcomings of conventional IVM system. Materials and Methods: Immature ovine oocytes were retrieved from pre antral follicles of abattoir ovaries by aspiration and after washing in Htcm + %10 FBS were immediately classified in four gr...
[ 20 ] - P-114: Gene
[ 21 ] - P-116: Antioxidant Effects of MnTE and Catalase on Goat Sperm Motility Following Freezing and Thawing
Background: This study was carried out to evaluate the protective effects of a cell permeable antioxidant, Manganese (III) meso-tetrakis (N-ethylpyridinium-2-yl) porphyrin chloride (MnTE), alone or in combination with different concentration of Catalase on motility of frozenthawed goat semen. Materials and Methods: Fresh semen ejaculates were collected with artificial vagina from 3 goats, mixed...
[ 22 ] - P-197: Aberrant Gene Expression Profile in Blastocyst Embryos As Consequences of Assisted Reproductive Technologies
Background: Currently, ARTs are of indispensable importance in treatment of human infertility. While ARTs are considered to be safe, some studies suggest that ARTs manipulations may perturb the epigenetic information and subsequently the health of the offspring. To examine these events further, this study was conducted to assess the effect of superovulation, in vitro culture and vitrification o...
[ 23 ] - P-24: DGC-HOST Method: An Approach for Selection of Normal Sperm during ICSI
Background: During ICSI, motile sperm with normal morphology are selected and injected into oocyte. But integrity of chromatin is not observable. Therefore, researchers are trying for to present novel method of sperm selection with high percentage of normal chromatin. HOST test is a useful assay of functional integrity of the human sperm membrane. We decide to use HOST test after DGC for infert...
[ 24 ] - P-41: Evaluation of CAPZA3 Expression in Infertile Individuals Undergoing Intracytoplasmic Sperm Injection
Background: CAPZA3 is one of the actin-capping proteins believed to be play an important role in controlling of actin polymerization during spermiogenesis and has been shown to be peresent in mature sperm. Immunohistochemistry showed that human CAPZA3 is localized in the neck region of ejaculated sperm, with moderate and faint signals also detected in the tail and post-acrosome region respectiv...
[ 25 ] - P-57: Evaluation of HSPA2 in Fertile and Infertile Individuals
Background: Heat-shock protein A2 (HspA2) is a testisspecific member of the HSP70 family known to play a critical role in spermatogenic cell differentiation. HspA2 is correlated with sperm maturity, function and fertility, and diminished expression of HspA2 results in abnormal sperm maturity and infertility. The aim of this study was to compare expression of HspA2 in fertile and infertile indiv...
[ 26 ] - P-83: Which Novel Sperm Selection Method Is Functional? MACS-DGC or DGC-Zeta
Background: Selection of human spermatozoa prior to assisted reproduction is currently based on motility and morphology. Recently, Zeta method has been introduced for normal sperm selection based on sperm surface charges. In addition, one of the features of apoptosis is the externalization of phosphatidylserine (PS), which are normally present on the inner leaflet of the sperm plasma membrane. ...
[ 27 ] - P-92: Specific Activation Requirements of In Vitro Matured Sheep Oocytes following Vitrification-Warming
Background: Oocyte vitrification and assisted oocyte activation have increasingly important implications in assisted reproductive technology. However, an important area of concern with matured oocyte cryobiology is that elements of oocytes intimately involved in metaphase-II arrest may be modified by cryopreservation. In a comparison between different cellular characteristics of unvitrified, vi...
[ 28 ] - P-94: Mouse Embryo Vitrification Cannot Effect on Global DNA Methylation in Preimplantation Stage
Background: Embryo vitrification was effectively used for assisted reproductive techniques. Despite the undeniable benefits of vitrification, cooling and warming stress, and cytotoxicity of cryoprotectant may affect the DNA methylation that have an important role in gene activation and silencing. In the present study effects of 2-cell embryo vitrification on DNA methylation in hatched blastocys...
[ 29 ] - P-98: Effect of Mouse Embryo Vitrification on Histone Modifications
Background: Vitrification has been usually used as an assisted reproductive technology in animals and humans. This method needs high concentrations of cryoprotectants that can be toxic with high cooling degrees. Then, vitrification could be change histone modifications such as methylation and acetylation can performance as regulatory controls of gene transcription. So, the purpose of the presen...
[ 30 ] - P-112: Potential Applications of Sheep Oocytes As Affected by Vitrification and In Vitro Aging
Background: The present study was carried out to investigate how the interactions between aging, vitrification, and post-warming interval affect the credibility of sheep MII-oocytes for in vitro fertilization (IVF), intracytoplasmic injection (ICSI), and parthenogenetic activation (PA). Materials and Methods: The vitrified-warmed oocytes, either immediately (immediate group: IG) or two hours po...
[ 31 ] - P-118: Effect of Vitrification on Developmental Competence of Parthenogenetic Activation in In Vitro Matured Ovine Oocytes
Background: Cryopreservation of in vitro matured oocytes is a useful technique because the oocytes can be used for some assisted reproductive technologies. On the other hand, the cryopreservation of oocytes is an open problem as a result of their structural sensitivity to the freezing process. The purpose of this study was to evaluate the effect of vitrification on in vitro development of vitri...
[ 32 ] - P-121: Assessment of Microtubule and Nuclear Status at Different Intervals of Bovine In Vitro Oocyte Maturation
Background: Mammalian oocyte undergoes a series of structural nuclear modulations during maturation in order to obtain full competence to support fertilization and early embryonic development. Microtubules are major cytoskeleton components and have pivotal modulators for chromosomal movement, and it has been shown that partial or immature spindle organization may compromise correct ploidy of th...
[ 33 ] - P-130: Designing and Construction of An Appropriate Eukaryotic Expression Vector to Generate Soluble Form of Human Hyaluronidase Type PH20 in Cell Culture Feasible for Application in IVF and ICSI
Background: The hyaluronidases are the enzymes hydrolyze β-1, 4 glycosidic linkage of hyaluronan. Hyaluronan is a polymer consisting of a repeating disaccharide unit found in cumulus ovuforus complex, semen liquid and other tissue. Addition to hydrolyzing the hyaluronan, hyaluronidase can penetrate through the cumulus cells layer that surrounds the oocyte, thus it terms spreading factor. Moreov...
[ 34 ] - P-136: In Vitro Development of Ovine Oocytes Matured in A Chemically Defined Medium
Background: Despite great advances achieved in the field of in vitro oocyte maturation (IVM), almost all IVM media are supplemented with undefined component which not only pose the risk of pathogen transmission but also hinders our knowledge of molecular mechanisms of maturation. One of these components is serum that containing various concentration of unknown molecules affecting cellular proce...
[ 35 ] - P-175: Relationship between Dietary Fat Intake and Its Major Food Sources and Assisted Reproduction Parameters
Background: To examine the relative contributions of dietary fat intake level and its food sources and assisted reproduction parameters. Materials and Methods: A prospective study was conducted on 240 infertile women. In assisted reproduction treatment cycle, body mass index (BMI), physical activity (PA), calorie intake, fat consumption and major food sources were assessed. The number of retrie...
[ 36 ] - P-204: Evaluation of DPY19L2 Gene Deletion As A Major Cause of Globozoospermia, in Iranian Globozoospermic Infertile Men
Background: Male infertility is a Multifactorial syndrome encompassing a wide variety of disorders. In more than half of infertile men, the cause of their infertility is unknown (idiopathic) and could be congenital or acquired. Globozoospermia, also called round-headed spermatozoa, is a rare disease with incidence< 0.1% among male infertile patients. The most prominent feature of globozoospermi...
[ 37 ] - P-84: Phospholipase C Zeta: As an Index to Assess Fertilization Potential of a Semen Sample
Background: Failed fertilization post ICSI has been mainly attributed to the sperm’s inability to induce oocyte activation. Phospholipase C Zeta (PLCζ) is considered to be a primary factor for the induction of oocyte activation. The aim of this study was to quantitatively assess the expression of PLCζ in globozoospermic or individuals with previous low or failed fertilization in comparison to f...
[ 38 ] - P-4: Zaditen (Ketotifen), as Mast Cell Blocker, Improves Sperm Quality, Chromatin Integrity and Pregnancy Rate after Varicocelectomy
Background: Surgery is considered the most common choice for the treatment of male infertility with clinical varicocele. Increased numbers of mast cells (MCs) have been associated with different types of infertility, including varicocele. Despite there being different reports on improved fertility following administration of MC blockers, there is no report on the effect of a MC blocker on varic...
[ 39 ] - P-6: Flow Cytometry: Assessment of Protamine Deficiency by CMA3 Staining and Apoptotic Bodies
Background: Chromomycin A3 (CMA3) staining, with the slide method or fluorescence microscopy, is widely used for indirect assessment of protamine deficiency in a semen sample. Flow cytometry is the most suitable tool which to improve assessment accuracy, both in terms of statistical analysis and to prevent observer variation. This study provides a simple procedure to account for merocyanine 540...
[ 40 ] - P-11: DHR123: A Suitable Fluorescent Mitochondrial Probe for Assessment of H2O2 in Human Spermatozoa
Background: The objective of present study was to assess potential of DHR123 (dihydrorhodamine 123) fluorescent probe for measurement of H2O2 produced by human spermatozoa and comparing the results with DCFH-DA (2', 7'-dichlorodihydrofluorescein diacetate). Materials and Methods: Fluorescent intensity and percentage R123 and DCF positive sperm measured by flow cytometry. The suitable ...
[ 41 ] - P-12: Direct Evaluation of HspA2 in Fertile and Infertile Individuals
Background: Heat-shock protein A2 (HspA2) is a testis-specific member of the HSP70 family known to play a critical role in spermatogenic cell differentiation. HSPA2 is correlated with sperm maturity, function and fertility, and diminished expression of HSPA2 results in abnormal sperm maturity and infertility. The aim of this study was to assess maturity of ejaculated spermatozoa via comparing p...
[ 42 ] - P-19: Assessment of Apoptosis Marker in Novel Sperm Selection Procedure
Background: Sperm that bypasses natural apoptosis may find their way into semen. In order to avoid the insemination of such sperm during an intracytoplasmic sperm injection (ICSI) treatment, novel sperm selection procedures such as the Zeta procedure have been implemented. Therefore, the aim of this study was to evaluate whether this procedure, in addition to reducing the number of immature or ...
[ 43 ] - P-22: Time Dependent Effect of Post Warming Interval on Microtubule Organization, Meiotic Status, and Parthenogenetic Activation of Vitrified In vitro Matured Sheep Oocytes
Background: It is a common practice to rest vitrified-warmed matured oocytes for 1-3 hours, as a treatment to recover spindle and cytoskeleton, before commencing a further treatment. Vitrified-warmed matured oocytes, however, are very sensitive and may resume meiosis spontaneously during this recommended rest time. Therefore, the aim of this study was to assess spindle and chromosome status as ...