Sanaz Dastgheib
Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; and Student Research Committee, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
[ 1 ] - Optimization of RNA Extraction from Rat Pancreatic Tissue
Background: Optimized RNA extraction from tissues and cell lines consists of four main stages regardless of the method of extraction: 1) homogenizing, 2) effective denaturation of proteins from RNA, 3) inactivation of ribonuclease, and 4) removal of any DNA, protein, and carbohydrate contamination. Isolation of undamaged intact RNA is challenging when the related tissue contains high levels of ...
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