Mohammad Hosein Sanati
National Institute of Genetic Engineering and Biotechnology (NIGEB), Shahrak-e- Pajoohesh, 15th Km, Tehran-Karaj Highway
[ 1 ] - In Silico Perspectives on the Prediction of the PLP’s Epitopes involved in Multiple Sclerosis
Background: Multiple sclerosis (MS) is the most common autoimmune disease of the central nervous system (CNS). The main cause of the MS is yet to be revealed, but the most probable theory is based on the molecular mimicry that concludes some infections in the activation of T cells against brain auto-antigens that initiate the disease cascade.Objectives: The Purpose of this research is the...
[ 2 ] - Effects of Crocin on The Pituitary-Gonadal Axis and Hypothalamic Kiss-1 Gene Expression in Female Wistar Rats
Background: Saffron (Crocus sativus L.) is traditionally used as a spice for coloring and flavoring in cooking in some countries. One of the main component of saffron is Crocin. Recent researches have shown Crocin has various pharmacological effects. The aim of this study was to assess the effect of Crocin on the Pituitary-Gonadal Axis and Kiss-1 gene expression in hypothalamus and also ovarian...
[ 3 ] - P-22: Codon Optimization of Coagulation Factor IX and Cloning in to The Chinese Hamster Ovary Cells
Background Human coagulation factor IX is a 57kDa plasma serine protease made in Liver which plays a vital role in the blood coagulation cascade. FIX deficiency causes severe disorder Hemophilia B or Christmas disease. Nowadays, recombinant proteins have important roles in treatment of diseases. Although, cultivated mammalian cells because of their ability for producing properly folded protein ...
[ 4 ] - P-98: The Effect of Primary pH Values of Medium Culture on Expression of Human Follicle Stimulating Hormone in Recombinant Chinese Hamster Ovary Cells.
Background One of the common gonadotropin hormones used for infertility treatment is recombinant Follicle stimulating hormone (rFSH). pH is an environmental factor that can affect production of recombinant proteins with no imposing cost. Culture pH is known as a parameter that significantly affects cell growth and protein production. In this study, tried to investigate the effect of different p...
[ 5 ] - P-122: The Effect of Beta Globin Intron on Human LH Hormone Expression in CHO Cells
Background Luteinizing hormone (LH) is a heterodimeric glycoprotein composed of alpha and beta subunits.This hormone is secreted from the pituitary gland. LH, in women triggers Menstrual cycle and ovulation. In men, LH stimulates production of testosterone, which plays a specialized role in sperm production. Up to day, LH hormone have produced in different ways such as codon optimization and al...
[ 6 ] - P-124: Comparative Study of The Effect of Fetal Bovine Serum Concentrations on Expressing of Human Follicle Stimulating Hormone.
Background Follicle stimulating hormone (FSH) produced in recombinant Chinese hamster ovary (CHO) cells used for infertility treatment. Fetal Bovine Serum (FBS) in known as a factor in medium culture that provides the tolerance towards physiochemical variations and protects cells against apoptosis; on the other hand, FBS is the most expensive component in medium culture and collection that caus...
[ 7 ] - P-127: The Effect of Beta Globin Intron on Human FSH Hormone Expression in CHO Cells
Background Follicle stimulating hormone (FSH)- a hetrodimeric glycoprotein- is secreted by pituitary gland. This hormone stimulates growth and maturation of the follicles in females and sperms in male. Up to now, glycoprotein hormones such as FSH have produced in different cell lines. Among of the mammalian expression systems, the Chinese hamster ovary cells (CHO) have taken into consideration ...
[ 8 ] - Application of the Novel CRISPR / Cas Technology in Diagnosis of COVID-19 Strains
The novel Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has caused respiratory infection, resulting in more than two million deaths globally and hospitalizing thousands of people until 2021. A considerable percentage of the SARS-CoV-2 positive patients are asymptomatic or pre-symptomatic carriers, facilitating the viral spread in the community by their social activities. Hence, i...
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