بوالحسنی, اعظم
بخش هپاتیت و ایدز، انستیتوپاستور ایران، تهران، ایران
[ 1 ] - آنالیز پلی مورفیسم ژنCTLA-4 به عنوان مارکر توسعه مهارکننده ها در بیماران ایرانی مبتلا به هموفیلی نوع A
سابقه و هدف: بروز مهارکننده علیه فاکتور انعقادی VIII اصلیترین مشکل درمان بیماران مبتلا به هموفیلی A است. اخیرا ارتباط پلی مورفیسم های برخی از ژن های دخیل در سیستم ایمنی با توسعه مهارکنندهها مشخص شده است. گزارش ها نشان داده اند که آنتی ژن 4 وابسته به لنفوسیت T سیتوتوکسیک (CTLA-4) نقش مهمی در تنظیم فعالیت سلولT برعهده داشته و بنابراین پلیمورفیسم ژن CTLA-4 با استعداد ژنتیکی به بیماری ها...
[ 2 ] - رنگ آمیزی معکوس: روشی مؤثر برای تخلیص پروتئین HIV-1 Nef در سیستم بیان پروکاریوتی
سابقه و هدف: پروتئین Nef، یکی از پروتئین های تنظیمی ویروس HIV، دارای اپیتوپ های حفاظت شده متعددی است که در افراد آلوده به ایدز پاسخ های ایمنی قدرتمندی علیه آن ها مشاهده شده است. بنابراین، پروتئین Nef کاندید مناسبی برای تولید واکسن می باشد. هدف این پژوهش کلونینگ و بیان پروتئین Nef در سیستم بیان پروکاریوتی و تخلیص آن به روش رنگآمیزی معکوس می باشد. مواد و روش ها: توالی کدکننده پروتئین Nef ...
[ 3 ] - Immunostimulants: Types and Functions
Immunomodulators are natural or synthetic materials that regulate the immune system and induce innate and adaptive defense mechanisms. These substances are classified into two types, immunostimulants and immunosuppressants. Immunostimulants can enhance body's resistance against various infections through increasing the basal levels of immune response. These agents could increase the oxidative a...
[ 4 ] - HSP Roles as Biomarkers and Antigens in Bacterial and Viral Infections
Diagnosis of infectious diseases remains an important issue in medical science. Identification of biomarkers can be used to predict early infections. Recently, heat shock proteins (HSPs) have been known as the conserved compounds expressed under stress conditions in both prokaryotic and eukaryotic systems. These proteins act as molecular chaperones. Several studies showed the increased levels o...
[ 5 ] - Synthesis and characterization of physicochemical and immunological properties of recombinant NS3-G2 dendrimer conjugate
Introduction: An effective vaccine against HCV infection is not available. The non-structural protein 3 (NS3) of the virus as an important immunogenic candidate has been utilized in various modules. Nanostructured polymers have been recently used for efficient vaccine and drug delivery. The aim of the current study was the synthesis of rNS3-G2 conjugate and preliminary evaluation of its immunog...
[ 6 ] - Different protein expression systems can influence the direction of the immune responses against HCV core protein in animal model
Introduction: Hepatitis C virus (HCV) infection is a major public health problem which influences about 170 million people worldwide. Different types of vaccines have been designed using HCV structural proteins to control the viral infection. The core nucleocapsid protein is one of the most conserved proteins and a desirable target for HCV vaccines, especially the protein-based vaccines. In cur...
[ 7 ] - DNA-Based Vaccine Is More Efficient than Non-Pathogenic Live Vaccine for the Prevention of HPV16 E7-Overexpressing Cancers
Introduction: Vaccinology provides promising approaches for the control of various infectious diseases. Among different strategies, DNA vaccines offer attractive research opportunities for development of vaccines for induction of antigen-specific immunity owing to their stability, simplicity of delivery, safety and cost effectiveness. However, there is a need to increase their potency by the ...
[ 8 ] - نقش‌های پروتئین هسته‌ای HMGB1 و دمین‌های اصلی آن در زمینه‌های مختلف بیولوژی
پروتئین(High mobility group box 1) HMGB1، پروتئین داخل سلولی است که به هسته انتقال مییابد و بیان ژنها را تنظیم میکند. پروتئین HMGB1 به عنوان یک پروتئین چند عملکردی نقش خود را از طریق نوترکیبی، تنظیم رونویسی و التهاب ایفا میکند. پروتئین HMGB1 به کیناز وابسته به سایکلین مانند CDK2 متصل میشود که رونویسی ژنهای مرتبط با پیشرفت چرخه سلولی را کنترل میکند. بهعلاوه HMGB1 به عنوان سایتوکاین جدید ...
[ 9 ] - Expression and Purification of HCV Core and Core-E1E2 Proteins in Different Bacterial Strains
Background: Hepatitis C virus (HCV) is a main public health problem causing chronic liver infection and subsequently liver cirrhosis and lethal hepatocellular carcinoma (HCC). Vaccination based on HCV capsid proteins has attracted a special interest for prevention of viral infections. The core protein is a basic and evolutionary most conserved protein, which regulates the cellular processes rel...
[ 10 ] - A Shot at Dendritic Cell-Based Vaccine Strategy against HIV-1
Introduction: Despite considerable efforts to control AIDS pandemic, it is still one of the significant infectious concerns worldwide. The advance in medical research has led to the development of highly active antiretroviral therapy with a considerable effect to suppress the disease. However, an effective vaccine capable of eradication the HIV pandemic is not available yet. Failure to develop ...
[ 11 ] - In vitro Delivery of HIV-1 Nef Antigen by Histidine-rich nona-arginine and Latarcin 1 peptide
Introduction: The Nef accessory protein is an attractive antigenic candidate in the development of HIV-1 DNA- or protein-based vaccines. The most crucial disadvantage of DNA and protein-based vaccines is their low immunogenicity, which can be improved by cell-penetrating peptides (CPPs) as effective carrier molecules. Methods: In this study, the HIV-1 Nef protein was generated in the Escherichi...
[ 12 ] - Evaluation of MALAT-1 and HOTAIR LncRNAs Expression Levels in Incidence of Hemophilia
Background and purpose: LncRNAs are a group of non-coding RNAs with more than 200 nucleotides involved in transcriptional and post-transcriptional regulation of gene expression, that have a potential role in diagnosis, prevention and treatment of some disorders such as cancers. There is paucity of information about their involvement in hemophilia disease. This study aimed at discovering the rel...
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