M. Afsharnasab
[ 1 ] - The comparative effects of dietary supplementation with Pediococcus acidilactici and Enterococcus faecium on feed utilization, various health-related characteristics and yersiniosis in rainbow trout (Oncorhynchus mykiss Walbaum, 1792)
This study examined the effects of feeding of two dietary probiotics on growth performance and feed utilization, intestinal colonization and related health criteria, biochemical parameters, hematological indices, and immune parameters and protection against Yersinia ruckeri in Oncorhynchus mykiss during a period of 56 days. The study included 3 groups:1(a control group of fish, 2) a group of fi...
[ 2 ] - Immunological findings in shrimp Litopenaeus vannamei exposed to attenuated WSSV vaccine produced by Gamma irradiation
In this research, White spot syndrome virus (WSSV) with 105.4LD50.mL-1 inactivated with gamma irradiation at a dose rate of 14-15 kGy.sec-1 was used as a vaccine. Three hundred and sixty shrimp of Litopenaeus vannamei, medium body weight (MBW) 1.0±0.17g), were prepared and experimentally exposed. They were distributed among four groups, inclusing vaccinated-virus exposed (T1), vaccinated-nonvir...
[ 3 ] - The effect of created hemolymph apoptosis on WSSV Gama-vaccinated shrimp, Litopenaeus vannamei in WSSV disease control
White spot syndrome virus (WSSV) is the causative agent responsible for huge-shrimp viral epidemics in shrimp farms throughout the world. Our study was aimed to determine the effect of WSSV Gamma-vaccinated Litopenaeus vannamei on the occurrence of apoptosis. One thousand and twenty PL15 were randomly distributed among 2 treatments and two control groups. Gama-Vaccinated shrimp and non-Gamma-va...
[ 4 ] - The Status of white spot syndrome virus (WSSV) in Islamic Republic of Iran
White spot syndrome virus (WSSV), the causative virus of disease, is found in most shrimp farming areas in Iran, and it cause large economic losses to the shrimp farming industry. Shrimp aquaculture is an important industry in Iran and plays an important role in aquaculture production. The shrimp production in 2012 was more than 12000 metric tonnes and it is estimated it will reach 20000 tonnes...
[ 5 ] - Hemocytosis, a new disease in cultured shrimp, Fenneropenaeus indicus
Certain parasites can cause severe diseases in shrimp. Among these, protozoa are very important. The objective of our study was to describe the signs, prevalence, and intensity of hemocytosis, a new disease in shrimp, Fenneropenaeus indicus. The experimental design included three groups, one treatment as infected shrimp or group B and 2 controls with non-infected but exposure to White Spot Synd...
[ 6 ] - Rate (ROI) and severity (SOI) of infection of white spot disease in cultured and captured Penaeidshrimps in the Persian Gulf using histopathology and polymerase chain reaction
White Spot Disease (WSD) is well known as a widespread viral disease in shrimps from 1992. Many studies focused on morphological, histopathological and epidemiological characteristic and pathogenecity of the disease but less on the determination of the severity of WSD using the histopathological features in target tissues. A generalized scheme for assigning a numerical qualitative value to sev...
[ 7 ] - Experimentally pathogenesis of Aeromonas hydrophila in freshwater Crayfish (Astacus leptodactylus) in iran
In this research the affect of A. hydrophila on pathogenesis of Crayfish A. leptodactylus was carried out. This study was designed in three groups as treatments and one group as control in triplicate with 20 Crayfish in each glass aquarium. The treatments have exposed to A. hydrophila. The concentrations of the bacteria in treatments were , and ...
[ 8 ] - Titration of the Iranian White Spot Virus isolate, on Crayfish Astacus leptodactylus and Penaeus semisulcatus
White Spot Virus (WSV) is currently the most serious viral pathogen of shrimp worldwide it causes mortality up to 100% within 7-10 days in commercial shrimp farms. Infected Indian white shrimp Fenneropenaeus indicus samples were collected from Guatr shrimp site in Sistan and Baluchestan province in south of Iran and WSV infection was confirmed by Nested PCR. WSV was isolated from infected shr...
[ 9 ] - Prevalence and intensity of internal parasitic helminthes infected Black sole fish, Brachirus orientalis (Bloch and Schneider, 1801) in the Persian Gulf
This study was conducted to identify intestinal helminthes parasites. The helminthes fauna is collected from body cavity and intestines of 108 specimens of Brachirus orientalis which were obtained from Abadan port (waterfront) and investigated during the years 2009-2010. Most of the 4 species of helminthes were found in the intestine and body cavity of the examined fishes. The helminthes found ...
[ 10 ] - Detection and identification of white spot syndrome virus (WSSV) and infectious hypodermal and hematopoietic necrosis virus (IHHNV) of Litopenaus vannamei from Bushehr and Sistan and Baloochestan provinces (Iran), during 2009-2010
The first time the white spot disease (WSD) and IHHNV were reported in Iran was in 2004 and 2008, respectively in Bushehr Province. In Sistan and Balochestan province only the WSD was reported in 2008.The aim of this study was detecting these two viral diseases in these provinces, during December 2009 to April 2010. A total of 364 samples were collected according to suspected gross signs from...
[ 11 ] - The effect of different salinities on mortality and histopathological changes of SPF imported Litopenaeus vannamei, experimentally exposed to White Spot Virus and a new differential hemocyte staining method
Shrimp farmers have been suffering from loss of production due to White Spot Disease (WSD) in the last decades. A few studies focused on the environmental factors predisposed WSD. Our study aimed to investigate the effects of the different salinities on occurrence of WSD. The treatments were 30 ppt, 40 ppt and 50 ppt in triplicate and corresponding untreated control groups. White Spot Virus (...
[ 12 ] - Immunological and histopathological changes in Penaeus semisulcatus challenged with Vibrio harveyi
Two-hundred and sixty five green tiger shrimp juveniles (Penaeus semisulcatus) with the average weight of 7-12g were collected from Helleh farms in Bushehr province and transported to Iran Shrimp Research Center of Bushehr in October, 2009. The juveniles were acclimated for two weeks. The experiment was designed in three treatments (named 3, 4 and 5) and two controls (named 1 and 2) in triplic...
[ 13 ] - Survey on fungal, parasites and epibionts infestation on the Astacus leptodactylus (Eschscholtz, 1823), in Aras Reservoir West Azarbaijan, Iran
A total of 394 (255 males,139 females) live freshwater crayfish Astacus leptodactylus from four stations of Aras reservoir in West Azarbaijan Province (North-Western Iran) were studied during the winter until early autumn of 2009 for the presence of parasites, Epibionts and Fungal agents. Parasitological surveys were carried out on gills exoskeleton and internal organs, mycological examinations...
[ 14 ] - Effect of different pH and salinity levels on the viability of Penaeus monodon baculovirus (MBV) in Penaeus semisulcatus
The virucidal effect of pH and salinity on Penaeus monodon baculovirus (MBV) was investigated by water borne inoculated methods exposing PL12 Penaeus semisulcatus for 10 hours and observing for 10 days at temperature 28- 30°C. For this purpose, one thousand virus free PL12 of P. semisulcatus were divided into five groups and each group placed in a 10 L plastic basin containing chlorine-treated ...
[ 15 ] - Effect of different pH and salinity levels on the viability of Penaeus monodon baculovirus (MBV) in Penaeus semisulcatus
The virucidal effect of pH and salinity on Penaeus monodon baculovirus (MBV) was investigated by water borne inoculated methods exposing PL12 Penaeus semisulcatus for 10 hours and observing for 10 days at temperature 28- 30°C. For this purpose, one thousand virus free PL12 of P. semisulcatus were divided into five groups and each group placed in a 10 L plastic basin containing chlorine-trea...
Co-Authors