Abdolhossein Shahverdi

[ 1 ] - Function of vitrified mouse ovaries tissue under static magnetic field after autotransplantation

This study was designed to investigate the effects of applying 1 mT static magnetic field (SMF) during the vitrification process, on the viability of ovarian follicles after vitrification-warming and autotransplantation. The study was conducted in two phases. In the first phase, ovaries of female NMRI mice (6 to 8 weeks old) were randomly divided into three groups: 1- Freshly isolated ovaries f...

[ 2 ] - اثرات مدت تنش‌ القایی اکسیداتیو ملایم پیش از انجماد بر کیفیت اسپرم گاو بعد از فرآیند انجماد-ذوب

هدف: هدف از این مطالعه بررسی اثرات زمان تنش‌ القایی اکسیداتیو ملایم با استفاده از نیتریک اکساید پیش از انجماد بر کیفیت اسپرم گاو پس از فرآیند انجماد- ذوب بود. مواد و روش‌ها: در این آزمایش، زمان تعادل پیش از انجماد 120 دقیقه در نظر گرفته شد. مقدار یک میکرومول نیتریک اکساید در زمان صفر (T0)، 45 دقیقه (T45)، 90 دقیقه (T90) و 120 دقیقه (T120) دوره سرد سازی پیش از انجماد به رقیق‌کننده اضافه شد. فرا...

[ 3 ] - همسانه‌سازی ژن زیرواحد بتا هورمون گونادوتروپین جفت انسانی در پی انتقال ژن به اسپرم خروس

هدف از این مطالعه، همسانه‌سازی‌زیرواحد بتای هورمون گونادوتروپین جفت انسانی در ناقل‌ مناسب بود که بتواند از طریق اسپرم در تولید طیور تراریخت به کار گرفته شود. به این منظور زیرواحد بتای این هورمون به کمک یک جفت آغازگر اختصاصی تکثیر و درناقل T همسانه‌سازی شد. فرآیند ترانسفورماسیون پلاسمید نوترکیب در سلول‌های مستعد اشریشیاکلی انجام گرفت و کلونی‌های دارای پلاسمید نوترکیب به‌وسیله PCR انتخاب شدند. صح...

[ 4 ] - The in vitro effect of chick embryo extract on mice pre-antral follicles

Chick embryo extract (CEE) contains a variety of growth factors which may improve in vitro follicle growth. Therefore, the effect of CEE on mouse pre-antral follicle culture was evaluated. Different percentages of CEE (0, 0.50%, 1.00%, 5.00% and 10.00%) were added to culture medium. Hence, the osmolarity of media was measured. Pre-antral follicles with diameter of 120-150 μm were isola...

[ 5 ] - P-19: The Effect of Mice Maternal Diet Supplemented with Omega-3 Fatty Acids on The Testicular Structure of Offspring: Stereological Study

Background Despite the bulk of studies on fatty acid supplementation in maternal diet and confirmed the positive effects on brain and vision, these effects on offspring reproductive organs have not been tested. The aim of the present study was to stereological evaluate the effect of feeding Fish Oil (FO) for mothers on the testis structure of offspring. MaterialsAndMethods Sixty mature female N...

[ 6 ] - Fatty Acid Profles of Ram's Sperm after Removing Some Fatty Acid Sources from the Diets and Persistency of Fatty Acids in Sperm

[ 7 ] - Comparison of Neonatal and Adult Mice-derived Sertoli Cells in Support of Expansion of Mouse Spermatogonial Stem Cells In vitro

[ 8 ] - Evaluation of Embryos Derived from in vitro Fertilized Oocytes Reconstructed by Meiosis-II Chromosome Transplantation from Aged Mice to Ooplasms of Young Mice

Background To assess embryos derived by the transfer of meiosis-II chromosomes (M-II-t) from aged mice oocytes into ooplasms from younger mice to overcome the problem of age-related decline in female fertility. MaterialsAndMethods The developmental capacity karyotype and ultrastructure of reconstructed oocytes derived from meiosis-II chromosome transplantation from aged mice into the ooplasms o...