Maral Rahimzadeh
Department of Nanobiotechnology, School of Biological Sciences, Tarbiat Modares University, Tehran, Iran
[ 1 ] - Impact of heat shock step on bacterial transformation efficiency
CaCl2 treatment followed by heat shock is the most common method for artificial transformation. Here, the cells were transformed using CaCl2 treatment either with heat shock (standard protocol) or without heat shock (lab protocol) to comprehend the difference in transformation efficiency. The BL21 strain of Escherichia coli (E. coli) was being susceptible using CaCl2 treatment. Some Cells were ...
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