Alireza Afsharifar
Plant Virology Research Center, College of Agriculture, Shiraz University, Shiraz, Iran
[ 1 ] - Evolutionary features of 8K (KDa) silencing suppressor protein of Potato mop-top virus
The cysteine-rich 8K protein of Potato mop-top virus (PMTV) suppresses host RNA silencing. In this study, evolutionary analysisof 8K sequences of PMTV isolates was studied on the basis of nucleotide and amino acid sequences. Twenty-one positively selected sites were identified in 8K codingregions. Recombination events were found in the 8K of PMTV isolates with a rate of 1.8. Totally 30 haplotyp...
[ 2 ] - Cap analog and Potato virus A HC-Pro silencing suppressor improve GFP transient expression using an infectious virus vector in Nicotiana benthamiana
Transient expression of proteins in plants has become a choice to facilitate recombinant protein production with its fast and easy application. On the other hand, host defensive mechanisms have been reported to reduce the efficiency of transient expression in plants. Hence, this study was designed to evaluate the effect of cap analog and Potato virus A helper component proteinase (PVA HC-Pro) o...
[ 3 ] - کشت بافت و تراریزش نارنج (Citrus aurantium) با استفاده از ژن کد کننده ی پوشش پروتئینی ویروس تریستزای مرکبات (CTV)
در این پژوهش به منظور به دست آوردن گیاهان تراریخته ی نارنج، از ریزنمونه های اپی کوتیل و هیپوکوتیل نارنج استفاده و بهبود اندام زایی مستقیم و تولید شاخساره در ریزنمونه ها با استفاده از تیمارهای مختلف بررسی شدند. تراریزش از طریق همکشتی ریزنمونه ها با Agrobacterium tumefaciens سویه EHA 105 حامل وکتور pFGC 5941 و ژن کد کننده ی پوشش پروتئینی ویروس تریستزای مرکبات (CTV) انجام شده. پس از تراریزش، ریزنم...
[ 4 ] - New Hosts and Comparison of Biological and Molecular Characteristics of Fig, Mulberry, and Citrus Isolates of Hop Stunt Viroid in Iran
Abstract Background and Aims: Hop stunt viroid (HSVd, genus Hostuviroid, family Pospiviroidae) has a wide host range among trees and herbaceous plants. The objectives of this study were to compare biological and physical characteristics of three isolates of HSVd from mulberry, fig and citrus and search for other hosts of this viroid in Iran. Materials and Methods: Plant samples were col...
[ 5 ] - Induction of Immune Response in Animal Model Using Recombinant Anti-NDV Vaccine
Background: Newcastle disease is a major avian disease that causes enormous economic loss in poultry industry. There have been a number of reports on the suitability of plant-based recombinant vaccine against this disease. Fusion (F) and hemagglutinin-neuraminidase (HN) epitopes of the Newcastle disease virus (NDV) represent the major immunogenic sites for development of recomb...