M. Haghkhah
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
[ 1 ] - Phenotypic characterizationand PCR-ribotyping of Pseudomonas fluorescensisolates, in tracking contamination routes in the production line of pasteurized milk
Contamination sites of Pseudomonas fluorescenswere traced in the production line for milk pasteurization in a large dairy plant in Shiraz, Iran. Samples of raw and pasteurized milk were collected at six sites along the line. All milk samples were incubated at 7°C until the aerobic plate count had reached 10 6 -10 7 cfu mL-1. Colonies were picked randomly and identified. No growth of gram negati...
[ 2 ] - Cloning and characterization of MAP2191 gene, a mammalian cell entry antigen of Mycobacterium avium subspecies paratuberculosis
The aim of this study is to identify, clone and express a Mycobacterium avium subsp. paratuberculosis specific immunogenic antigen candidate, in order to develop better reagents for diagnosis and vaccines for the protection of the host. Therefore, MAP2191 gene (a member of MAPmce5 operon) from MAP, was isolated and characterized by Bioinformatics tools and <e...
[ 3 ] - جداسازی مایکوباکتریوم ایویوم زیر گونه پاراتوبرکولوزیس از نمونه‌های مشکوک و تائید آن به روش Nested-PCR
پاراتوبرکلوزیس (بیماری یون) بیماری مزمن گرانولوماتوزی روده باریک توسط مایکوباکتریوم ایویوم تحت گونه پاراتوبرکولوزیس (MAP) ایجاد می‌شود. در کنترل بیماری، مهم‌ترین اقدام تشخیص و جداسازی حیوانات آلوده می‌باشد لذا هدف این بررسی جداسازی عامل این بیماری از دام‌های مشکوک و در ادامه تائید جدایه‌ها با استفاده از تست‌های مولکولی بود. برای این منظور ۱۴۲ نمونه مشکوک ارسالی از استان‌های اصفهان، البرز، تهران...
[ 4 ] - Isolation, Molecular Identification And Genomic Pattern of Mycobacterium Bovis Isolates Collected From Tuberculin-Positive Cattle in Infected Farms of Shiraz
Background: Mycobacterium bovis is the main cause of tuberculosis in cattle. At the global scale and also in Iran, the most frequent currently-in-use method in the detection of infected cattle is tuberculination. Objective: The present study was aimed to improve our genomic knowledge of Mycobacterium bovis population structure in cattle farms of Shiraz. Materials and Methods: Fifty pathologic...
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