S. Malekzadeh-Shafaroudi
Department of Biotechnology and Plant Breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
[ 1 ] - Expression of HA1 antigen of H5N1 influenza virus as a potent candidate for vaccine in bacterial system
The impending influenza virus pandemic requires global vaccination to prevent large-scale mortality and morbidity, but traditional influenza virus vaccine production is too slow for rapid responses. In this study, bacterial system has been developed for expression and purification of properly folded HA1 antigen as a rapid response to emerging pandemic strains. Here, a recombinant H5N1 (A/Indone...
[ 2 ] - Characterization of Iranian Nonaflatoxigenic Strains of Aspergillus flavus Based on Microsatellite-primed PCR
Out of fifty-two Iranian nonaflatoxigenic strains of Aspergillus flavus, which were collected from various substrates (soil and kernel) and sources (peanut, corn and pistachio), fifteen representatives were selected according to their different geographical origins (six provinces: Guilan and Golestan, Ardebil, Fars, Kerman and Semnan) and vegetative compatibility groups (VCGs, IR1 to IR15) for ...
[ 3 ] - Gene deletion patterns in non-aflatoxigenic isolates of Aspergillus flavus
Fifteen non-aflatoxigenic strains of Aspergillus flavus, represent a wide range of geographic regions of Iran (six provinces include Fars, Ardebil, Guilan, Golestan, Kerman and Semnan) and vegetative compatibility groups (VCGs), were collected from corn (Zea mays L.), peanut (Arachis hypogaea L.) and pistachio (Pistachia vera L.) soils and kernels, were screened for the presence of aflatoxin bi...
[ 4 ] - اثر رشد گلدانی پنبه تراریخته Bt روی باکتری‌های ریزوسفری و غیرریزوسفری خاک
در این مطالعه، پنبه تراریخته Bacillus thuringiensis (Bt)نسل T2 (لاین 61) و پنبه غیرتراریخته برای بررسی پویایی باکتری‌ها در طی دوره‌های رشد و نمو ( نونهالی، تشکیل کاسبرگ، گلدهی و تشکیل غوزه) با استفاده از دو روش قابل کشت (شمارش کلنی‌های زنده) و غیرقابل کشتPCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) مورد ارزیابی قرار گرفت. تعداد باکتری‌هایعملکردی تثبیت‌کننده نیت...
[ 5 ] - Comparative volatile composition, antioxidant and cytotoxic evaluation of the essential oil of Zhumeria majdae from south of Iran
Objective(s): The purpose of this study was to evaluate variations in yields, volatile composition and biological activities of essential oils (EOs) obtained from the aerial parts of Zhumeria majdae collected from five localities of the south of Iran. Materials and Methods: The EOs were analyzed using gas chromatography and gas chromatography-mass spectrometry techniques. The antioxidant activi...
[ 6 ] - Cultivation Effect of Chitinase-Transgenic Cotton on Functional Bacteria and Fungi in Rhizosphere and Bulk Soil
Background: In consideration for the increasing widespread use of genetically modified (GM) crops, one of the important issues for assessment is the effect of GM crops on soil microbial communities Objectives: In this study, T2 chitinase-transgenic cotton (line #57) and its non-transgenic line were investigated for bacterial and fungal dynamics...
[ 7 ] - Expression of an epitope-based recombinant vaccine against Foot and Mouth Disease (FMDV) in tobacco plant (Nicotiana tabacum)
Regarding high potential of green plants for development of recombinant vaccines, this research was conducted to evaluate expression of a novel recombinant vaccines against Foot and Mouth Disease (FMDV) in tobacco plant. For this purpose, a synthetic gene encoding 129-169 amino acids of foot and mouth disease virus capsid protein VP1 was transferred to tobacco plant via Agrobacterium-mediated g...
[ 8 ] - Study on Combining Ability and Gene Effects Estimation in Some Sweet Corn Inbred Lines (Zea mays L. var saccarata) by Line × Tester Method
In breeding programs determination of gene effects and general and specific combining ability for screening of test crosses is necessary. In order to estimate the genetic variance components and the general and specific combining ability of sweet corn lines, an experiment was conducted using 8 sweet corn S6 inbred lines (including 4 maternal and 4 paternal lines) by line × tester mating design ...
[ 9 ] - Identification of Genetic Sources of Resistance to the Seed-Born Leaf Stripe Disease of Barley (Pyneophera graminea Ito & Kurib)
Extended Abstract Introduction and Objective: Leaf stripe disease of barley is caused by the seed transmitted hemi-biotrophic fungus Pyneophera graminea which has spread in to important areas of barley production in Iran especially in Khorasan Razavi province. The fungus survives as a mycelium in the pericarp, the hull and the seed coat, but not in the embryo. During the seed generation, its g...
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