Valiollah Babaeipour
Department of Bioscience and Biotechnology, Malek Ashtar University of technology, Tehran, Iran
[ 1 ] - Enhanced Production of Insulin-Like Growth Factor I Protein in Escherichia coli by optimization of five key factors
Abstract Human insulin-like growth factor I (hIGF-I) is a kind of growth factor with clinical significance in medicine. The major objective of this study is over- production of recombinant human insulin-like growth factor I( rhIGF-I) through a developed process by recruiting effective factors in order to achieve the most recombinant protein. Up to now E. coli expression system has been widely us...
[ 2 ] - Overexpression of Recombinant Human Beta Interferon (rhINF-β) in Periplasmic Space of Escherichia coli
Human Interferon β (INF-β) is a member of cytokines family which different studies have shown its immunomodulatory and antiviral activities. In this study an expression vector was designed and constructed for expression of human INF-β-1b either in shake flasks and bench top bioreactor. The designed vector was constructed based upon pET-25b(+) with T7 promoter. Recombinant human beta interferon ...
[ 3 ] - Enhanced Production of Insulin-Like Growth Factor I Protein in Escherichia coli by optimization of five key factors
Abstract Human insulin-like growth factor I (hIGF-I) is a kind of growth factor with clinical significance in medicine. The major objective of this study is over- production of recombinant human insulin-like growth factor I( rhIGF-I) through a developed process by recruiting effective factors in order to achieve the most recombinant protein. Up to now E. coli expression system has been widely us...
[ 4 ] - Overexpression of Recombinant Human Beta Interferon (rhINF-β) in Periplasmic Space of Escherichia coli
Human Interferon β (INF-β) is a member of cytokines family which different studies have shown its immunomodulatory and antiviral activities. In this study an expression vector was designed and constructed for expression of human INF-β-1b either in shake flasks and bench top bioreactor. The designed vector was constructed based upon pET-25b(+) with T7 promoter. Recombinant human beta interferon ...
[ 5 ] - Dissolution and Regeneration of the Produced Nano Bacterial Cellulose of Food Industries Wastewaters by a Cost-Benefit Method
This paper applied a simple and cost-benefit method for the production of regenerated bacterial cellulose. The inexpensive production of cellulose with complex media derived from wastewater from food industries such as molasses adds a lot of contaminants to the produced bacterial cellulose, which puts a lot of challenges in cellulose purification. Therefore, the present study aimed...
[ 6 ] - خالص سازی هورمون رشد انسانی تولید شده به شکل محلول از سویه نوترکیب اشریشیاکلی در مقیاس آزمایشگاهی
سابقه و هدف: هورمون رشد انسانی یا سوماتوتروپین یک پلی پپتید تک زنجیره ای با وزن مولکولی 22 کیلودالتون دارای 191 واحد آمینو اسیدی است. هدف از این پژوهش، ارزیابی تولید و خالص سازی فرم محلول هورمون رشد انسانی نوترکیب در مقیاس آزمایشگاهی می باشد. مواد و روش ها: در این تحقیق از باکتری اشریشیا کلی حاوی پلاسمید pET32a(+)-Trx-His6-hGH استفاده ش...