ali Sharafi
Biotechnology Research Center, Zanjan University of Medical Sciences
[ 1 ] - Co -delivery of Sulforaphane and Curcumin with PEGylated Iron Oxide-Gold Core Shell Nanoparticles for delivery to breast cancer cell line
Co-delivery approach has been recommended to reduce the amount of each drug and to achieve the synergistic effect for cancer treatment. CUR and SF have antitumor effects, but their application is limited because of their low water solubility and poor oral bioavailability. To improve the bioavailability and solubility of SF and CUR, we performed an innovative co-delivery of SF and CUR with magne...
[ 2 ] - Simple protocol for plant regeneration of Lilium ledebourii using transverse thin cell layer
Transverse thin cell layer sections excised from in vitro scales of Lilium ledebourii were cultured on Murashige and Skoog (MS) medium supplemented with various plant growth regulators (PGRs) at different concentrations. Although, bulblets were produced on PGRfree MS medium during organogenesis, addition of 0.25 mg l-1 6-benzyladenine or 5.0 mg l-1 indole-3-acetic acid to the medium increased t...
[ 3 ] - Co -delivery of Sulforaphane and Curcumin with PEGylated Iron Oxide-Gold Core Shell Nanoparticles for delivery to breast cancer cell line
Co-delivery approach has been recommended to reduce the amount of each drug and to achieve the synergistic effect for cancer treatment. CUR and SF have antitumor effects, but their application is limited because of their low water solubility and poor oral bioavailability. To improve the bioavailability and solubility of SF and CUR, we performed an innovative co-delivery of SF and CUR with magne...
[ 4 ] - بررسی فعالیت ضد میکروبی نانوذرات نقره سنتز شده با ستفاده ا عصاره ایزوله اکتینومیست دریایی Nocardiopsis sp. AHA2 علیه پاتوژن‌های میکروبی
Background: Silver nanoparticles have been widely used in various biomedical applications and drug delivery systems. The biosynthesis of nanoparticles has been proposed as a cost effective environmental friendly alternative to chemical and physical methods. In the present study, synthesis of nanoparticles using a novel Nocardiopsis sp. AHA2 has been attempted. We used culture supernatant of Noc...
[ 5 ] - جداسازی اکتینومیست های دریایی رسوبات بین جزر و مدی ساحل دیلم و مطالعه خواص ضدقارچی آن ها
زمینه و اهداف: اکتینومیست های دریایی، باکتریهای گرم مثبت، شمار زیادی متابولیتهای ثانویه جدید تولید میکنند و منبع غنی از ترکیبات فعال زیستی ضدمیکروبی میباشند. از جمله منابع مهم اکتینومیستهای جدید، رسوبات دریایی هستند که بعنوان منبعی از آنتی بیوتیکهای جدید شناخته شدهاند. هدف از این تحقیق جداسازی اکتینومیست های دریایی تولید کننده ترکیبات فعال زیستی از رسوبات بستر ساحل دیلم و بررسی پتانسی...
[ 6 ] - تأثیر عصاره مخمر بر فعالیت آنتی‌اکسیدانی و تولید آلکالوئیدهای تروپانی در کشت ریشه‌های موئین بذرالبنج مشبک (Hyoscyamus reticulatus L.)
گونه‌های بنگ‌دانه (هیوسیاموس) ازجمله بذرالبنج مشبک (Hyoscyamus reticulatus L.) منابع غنی آلکالوئیدهای تروپانی، عمدتاً هیوسیامین و اسکوپولامین میباشند که به‌دلیل اثرات گشاد کردن مردمک چشم، ضد اسپاسم، آنتی‌کولینرژیک، آرام‌بخش و مسکن مورد استفاده قرار می‌گیرند. به‌دلیل ساختار شیمیایی پیچیده، این آلکالوئیدها از منابع طبیعی به‌طور عمده گیاهان تیره سیب‌زمینی بدست می‌آیند. القاء مسیرهای بیوسنتزی متابو...
[ 7 ] - Simple and sensitive high performance liquid chromatographic (HPLC) method for the determination of the apigenin from dried powder of Cosmos bipinnatus, Apium graveolens and Petroselinum crispum
Apigenin is best known as an irreversible inhibitor of monoamine oxidase (MAO), an intracellular enzyme associated with the outer membrane of mitochondria. The purpose of this study is to establish a reliable and quick method for the assignment of Apigenin in Cosmos bipinnatus, Apium graveolens and Petroselinum crispum by high performance liquid chromatography with ultraviolet detection (HPLC-U...
[ 8 ] - Cell Suspension Culture of Plumbago europaea L. Towards Production of Plumbagin
Background: Plumbagin is as an important bioactive secondary metabolite found in the roots of Plumbago spp. The only one species, Plumbago europaea L., grows wild in Iran. The therapeutic use of plumbagin is limited due to its insufficient supply from the natural sources as the plants grow slowly and take several years to produce quality roots. O...
[ 9 ] - افزایش بیان ژن h6h و تولید تروپان آلکالوئیدها در کشت ریشه‌های موئین گیاه دارویی بذرالبنج مشبک تهییج شده با عصاره‌ی قارچ فوزاریوم گرامینه‌روم
گیاه بذرالبنج مشبک Hyoscyamus reticulatus L.)) منبع غنی از آلکالوئیدهای تروپانی هیوسیامین و اسکوپولامین می باشد. در پژوهش حاضر، ریشه‌های موئین از ریزنمونه‌های کوتیلدون دو هفته‌ای، گیاه بذرالنج مشبک با استفاده از سویه A7 آگروباکتریوم رایزوژنز تهیه شدند. اثر غلظت‌های مختلف (صفر، 5، 10 و20 میلی‌گرم بر لیتر) عصاره قارچ فوزاریوم گرامینه‌روم در زمان‌های مختلف (24، 48 و 72 ساعت) بر میزان رشد، فعالیت آ...
[ 10 ] - Anti-Proliferative Properties, Biocompatibility, and Chemical Composition of Different Extracts of Plantago major Medicinal Plant
Background: To study the anticancer activity of Plantago major, ethanolic, methanolic and acetonic extracts were assessed on HCT116, SW480 and HEK-293 as control. Methods: The cytotoxic activity, biocompatibility and toxicity tests were evaluated by MTT assay, hemolysis and Artemia salina - LD50 (on mice) tests, respectively. The analysis of essential oil compounds was performed by GC-MS analys...
[ 11 ] - A comparative study of apigenin content and antioxidant potential of Cosmos bipinnatus transgenic root culture
Flavonoid derived components have been studied for their therapeutic properties. Apigenin has shown remarkable anti-oxidant and anti-inflammatory features; however having a reliable source of apigenin is necessary. In this study the amount of apigenin in flowers, roots, leaf and stems of three varieties of Cosmos bipinnatus: Dazzler, Xanthos and Sensation pinkie and also in transgenic root cult...
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