Hossein Vahidi

[ 1 ] - Simultaneous Optimization of the Production of Organic Selenium and Cell Biomass in Saccharomyces Cerevisiae by Plackett-Burman and Box-Behnken Design

Selenium (Se) as a vital trace element has many biological activities such as anti-inflammation and anti-oxidation. Selenomethionine as an organic selenium plays a vital role in the response to oxidative stress. At present, Saccharomyces cerevisiae is one of the best microorganisms that has the ability to accumulate selenium. Production of Seleno-yeast was done by growing Saccharomyces cerevisi...

[ 2 ] - Enhanced Production of Insulin-Like Growth Factor I Protein in Escherichia coli by optimization of five key factors

Abstract Human insulin-like growth factor I (hIGF-I) is a kind of growth factor with clinical signiﬁcance in medicine. The major objective of this study is over- production of recombinant human insulin-like growth factor I( rhIGF-I) through a developed process by recruiting effective factors in order to achieve the most recombinant protein. Up to now E. coli expression system has been widely us...

[ 3 ] - Biotransformation of Progesterone by Whole Cells of Filamentous Fungi Aspergillus brasiliensis

Microbial steroid biotransformation have found wide-reaching application for the production of more precious and functionalized compounds due to their high regio-and stereoselectivity. In this study, the possibility of using filamentous fungi Aspergillus brasiliensis cells in the biotransformation of progesterone (I), a C-21 steroid hormone was studied for the first time.The fungal strain was i...

[ 4 ] - Challenges to Design and Develop of DNA Aptamers for Protein Targets. I. Optimization of Asymmetric PCR for Generation of a Single Stranded DNA Library

Aptamers, or single stranded oligonucleotides, are produced by systematic evolution of ligands by exponential enrichment, abbreviated as SELEX. In the amplification and regeneration step of SELEX technique, dsDNA is conversed to ssDNA. Asymmetric PCR is one of the methods used for the generation of ssDNA. The purpose of this study was to design a random DNA library for selection of aptamers wit...

[ 5 ] - Overexpression of Recombinant Human Beta Interferon (rhINF-β) in Periplasmic Space of Escherichia coli

Human Interferon β (INF-β) is a member of cytokines family which different studies have shown its immunomodulatory and antiviral activities. In this study an expression vector was designed and constructed for expression of human INF-β-1b either in shake flasks and bench top bioreactor. The designed vector was constructed based upon pET-25b(+) with T7 promoter. Recombinant human beta interferon ...

[ 6 ] - Simultaneous Optimization of the Production of Organic Selenium and Cell Biomass in Saccharomyces Cerevisiae by Plackett-Burman and Box-Behnken Design

Selenium (Se) as a vital trace element has many biological activities such as anti-inflammation and anti-oxidation. Selenomethionine as an organic selenium plays a vital role in the response to oxidative stress. At present, Saccharomyces cerevisiae is one of the best microorganisms that has the ability to accumulate selenium. Production of Seleno-yeast was done by growing Saccharomyces cerevisi...

[ 7 ] - Enhanced Production of Insulin-Like Growth Factor I Protein in Escherichia coli by optimization of five key factors

Abstract Human insulin-like growth factor I (hIGF-I) is a kind of growth factor with clinical signiﬁcance in medicine. The major objective of this study is over- production of recombinant human insulin-like growth factor I( rhIGF-I) through a developed process by recruiting effective factors in order to achieve the most recombinant protein. Up to now E. coli expression system has been widely us...

[ 8 ] - Biotransformation of Progesterone by Whole Cells of Filamentous Fungi Aspergillus brasiliensis

Microbial steroid biotransformation have found wide-reaching application for the production of more precious and functionalized compounds due to their high regio-and stereoselectivity. In this study, the possibility of using filamentous fungi Aspergillus brasiliensis cells in the biotransformation of progesterone (I), a C-21 steroid hormone was studied for the first time.The fungal strain was i...

[ 9 ] - Challenges to Design and Develop of DNA Aptamers for Protein Targets. I. Optimization of Asymmetric PCR for Generation of a Single Stranded DNA Library

Aptamers, or single stranded oligonucleotides, are produced by systematic evolution of ligands by exponential enrichment, abbreviated as SELEX. In the amplification and regeneration step of SELEX technique, dsDNA is conversed to ssDNA. Asymmetric PCR is one of the methods used for the generation of ssDNA. The purpose of this study was to design a random DNA library for selection of aptamers wit...

[ 10 ] - Overexpression of Recombinant Human Beta Interferon (rhINF-β) in Periplasmic Space of Escherichia coli

Human Interferon β (INF-β) is a member of cytokines family which different studies have shown its immunomodulatory and antiviral activities. In this study an expression vector was designed and constructed for expression of human INF-β-1b either in shake flasks and bench top bioreactor. The designed vector was constructed based upon pET-25b(+) with T7 promoter. Recombinant human beta interferon ...