Alireza Rafiei
Department of Immunology, School of Medicine | Molecular and Cell Biology Research Center
[ 1 ] - Regulatory T Cells and Myeloid-Derived Suppressor Cells in Patients with Peptic Ulcer and Gastric Cancer
Background: Regulatory T Cells (Tregs) and Myeloid-Derived Suppressor Cells (MDSCs) are two main regulatory cells modulating the immune responses in inflammation and cancer. Objective: To investigate and compare Tregs and MDSCs in peptic ulcer and gastric cancer. Methods: Patients with dyspepsia were selected and divided into three groups of non-ulcer dyspepsia (NUD, n=22), peptic ulcer disease...
[ 2 ] - Cytokine Production in Hymenolepis Nana Infection
Background: Although many experimental studies provide convincing evidence that type II immunity is protective against helminths, recent data in mice demonstrate that Th1 is also impor-tant in some cestodes like Hymenolepis nana. Objective: To identify the role of Th1 and Th2 lymphocytes in immunity against H. nana, the levels of IL-12, IFNγ, IL-5, and IL-13 were de-termined in serum of humans ...
[ 3 ] - شگفتی های درمانی انجیر در قرآن و طب
Fig is one of the rare fruits mentioned in Holy Quran and also has a special status in traditional medicine. Fig is a part of health promoting Mediterranean- diet which is used as drug and food throughout the world too. This study was designed with an intention to provide complete review of fig status in religion, traditional medicine, pharmacological and medicinal compounds and ac...
[ 4 ] - زیتون و راز تاکید قرآن بر مصرف آن
Olive is one of the rare plants having special status in catechesis, holy Quran explicitly nominated it and our religious traditions declared its usage frequently. Olive is one of the folklore plants in Mediterranean coasts became a symbol because of high intake of olive and olive products. The present review article aimed to evaluate known effects of olive according to its p...
[ 5 ] - شگفتی‌های درمانی انجیر و رازهای نهفتۀ آن در قرآن و طب سنتی
سابقه و هدف: انجیر یکی از گیاهانی است که در قرآن از آن یاد شده است و همچنین جایگاه ویژه‌ای در طب سنتی دارد. انجیر به‌عنوان یکی از اجزای رژیم غذایی بهبود دهنده سلامتی مدیترانه که به‌طور گسترده در سراسر دنیا هم به‌عنوان دارو و هم به‌عنوان منبع غذایی مورد استفاده قرار می‌گیرد. این مطالعه با هدف ایجاد مروری کامل بر جایگاه انجیر در دین، طب سنتی، ترکیبات و فعالیت‌های دارویی و درمانی آن انجام شد. موا...
[ 6 ] - بررسی بیان ژن های T-bet و GATA-3 در کودکان مبتلا به آسم آلرژیک در مقایسه با گروه شاهد
Background and purpose: Allergic asthma is a chronic inflammatory disorder with increased inflammation and bronchial over react to stimulants. Th1 and Th2 cells are the main cells involved in the pathophysiology of asthma. The function of these cells is under the influence of T-bet and GATA3 transcription factors. This study aimed to investigate the impairment of immune responses in patients wi...
[ 7 ] - آثار تغذیه ای- درمانی انار از دیدگاه متون اسلامی، طب سنتی و طب مدرن
Background and purpose: Good nutrition is an important part of healthy lifestyle. Guidelines on nutrition are correlated with individual and public health and prevention or treatment of various diseases. Quran that covers all aspects of human life, provides guidance on nutrition too. Pomegranate is mentioned three times in Quran under the name of “Ar-Rumman”. The main aim of this st...
[ 8 ] - مروری بر مطالعات کوهورت مبتنی بر جمعیت در ایران
Background and purpose: Cohort studies are one of the best types of observational studies in investigating the causal relationship, diseases etiology, and determining the incidence and natural history of diseases. In recent decades cohort studies played a major role in identification of environmental, cardiovascular and cancers risk factors. Considering the important role of cohort studies in p...
[ 9 ] - مروری برکاربرد نانوذرات در رهش فاکتورهای رشد
Controlling the release of drugs and other therapeutic molecules has great importance in novel drug delivery. Nanoparticulate systems have the ability to control drug release and increase the presence of these compounds in blood flow and could convey them to a specific position. Growth factors are endogenous peptide which begins cellular signals to regulate cellular activities. The main problem...
[ 10 ] - نقش عوامل عفونی در پاتوژنز مالتیپل اسکلروزیس
Multiple Sclerosis (MS) is a complex autoimmune demyelinating disease of the central nervous system. Although the main cause of MS is still unknown, several risk factors may contribute in MS development. In the search for the etiology of MS, infectious agents, genetic factors, and/or their combination were believed to participate in MS occurrence. The association between MS and infectious disea...
[ 11 ] - اینترفرون ها در بیماری‌های خود ایمن: دوست یا دشمن
Interferons (IFNs) are pleotropic cytokines with strong anti-viral and immunomodulatory properties. Their function is dependent to signaling pathway upon binding to IFN receptor. Both pro- and anti-inflammatory effects are seen in interferons-related immune responses. While, interferons are in direct correlation with autoantibodies production during autoimmunity, they have protective effects. T...
[ 12 ] - انتخاب اختصاصی فاژهای حاوی قطعه آنتی‌بادی به کمک پروتئین A در تکنیک کتابخانه آنتی‌بادی فاژ
Background and purpose: Antibody phage display library is a powerful in vitro technology for production of recombinant antibody fragments against a wide variety of antigens. However, the presence of insert-free clones in the phage libraries limited the specific enrichment of antibody fragments in many studies. The aim of this study was to protein A-aided recovery of insert-containing phages in ...
[ 13 ] - ارزش غذایی و درمانی خرما از دیدگاه منابع اسلامی و طب سنتی و نوین
Background and purpose: The fruit of the date palm is highly nutrient which is mentioned in Holy Q,uran for several times. In addition to being a good energy source, date contains many micronutrients, therefore, it is recommended in Islamic and traditional medicine for health and prevention of diseases. In this study we reviewed the health benefits of date according to Holy Q,uran, and Islamic ...
[ 14 ] - بررسی ارتباط پلی مورفیسم ژن اینترلوکین-17 (IL-17F) با افزایش استعداد ابتلاء به سرطان معده
Background and purpose: Gastric cancer is one of the most common gastrointestinal cancers in the world. Gastric cancer is the most common cancer in north of Iran. Interleukin-17F (IL-17F) is a pro-inflammatory cytokine expressed by a novel subset of CD4+ Th cells (Th-17), and it causes the occurrence and strengthening the inflammatory response. This study aimed to assess the association betwe...
[ 15 ] - ارزیابی مقایسه‌ای مایع کیست هیداتیک با سرم جنین گاو در محیط کشت سلول فیبروبلاست موش صحرایی
Abstract Background and purpose: The most common serum used in culture media is fetal bovine serum (FBS). Given that FBS is costly, this study aimed at evaluating the hydatid cyst fluid (HCF) as a substitute for FBS in culture medium of fibroblast cells isolated from rat. Materials and methods: In this experimental study, skin dermal area from inguinal and abdomen of rat were used for fibro...
[ 16 ] - بررسی ارتباط پلی مرفیسم ژن Fas/CD95 و آدنوکارسینوم معده
Background and purpose: Apoptosis is a physiological mechanism of programmed cell death that dysregulation in this process may lead to carcinogenesis. Functional gene polymorphism in Fas can alter transcriptional activities and thus change the risks of cancer. A to G substitution in Fas -670 promoter region disrupts the binding site of STAT1 transcription factor. This study aimed to assess the ...
[ 17 ] - شناسایی اپی توپ های سلول T مربوط به آنتی ژن MPB51 مایکوباکتریوم توبرکلوزیس در موش های BALB/c
زمینه و هدف: سلول های T کمکی نوع یک (Thl) و لنفوسیت های CD8+T نقش به سزایی در برقراری حفاظت مقابل عفونت با مایکوباکتریوم توبرکلوزیس دارند. MPB51 یکی از پروتیین های ترشحی مایکوباکتریوم می باشد که باعث تحریک پاسخ های ایمنی هومورال و سلولی در طی عفونت با باسیل های سل می گردد. هدف از این مطالعه شناسایی اپی توپ های غالب لنفوسیت های T از مولکول MPB51 در موش های BALB/c می باشد.روش بررسی: این مطالعه ...
[ 18 ] - شناسایی اپی توپهای سلولهای CD4+ T در پروتئین ترشحی MPB51 مایکوباکتریوم توبرکلوزیس در موشهای C57BL/6
Introduction & Objective: Both CD4+ type 1 helper (Th1) cells and CD8+ T cells play effective roles in protection against Mycobacterium tuberculosis infection. DNA vaccine encoding MPB51 can induce Th1-type immune responses and protective immunity upon challenge with M.tuberculosis. This study address to identify T-cell immunodominant epitopes on MPB51 in C57BL/6 mice. Materials & Methods : ...
[ 19 ] - Identification of Mycobacterium tuberculosis CTL Epitopes Restricted by HLA-A*0201 in HHD Mice
CD8+ T cells are thought to play an important role in protective immunity to tuberculosis. The major histocompatibility complex class I subtype HLA-A*0201 is one of the most prevalent class I alleles, with a frequency of over 30% in most populations. HLA-A*0201 transgenic, H-2Db/mouse beta2-microglobulin double-knockout mice (HHD) which express human HLA-A*0201 but no mouse class I, was shown t...
[ 20 ] - Immune dysregulation in children with allergic asthma, a close relationship between IL-17 but not IL-4 or IFN-, and disease severity
Background : Allergic asthma is a chronic inflammatory disease of airway that often determined with degrees of inflammation, hypersensitivity, bronchial constriction and airway changes. Th1, Th2 and Th17 cells are the main cells involved in the pathophysiology of asthma. . Therefore, this study evaluated Th1, Th2, and Th17 cells functions by assessment the expression of INF-g, IL-4, and IL-17 g...
[ 21 ] - Anticancer Effect of Bovine Lactoferrin on Human Esophagus Cancer Cell Line
Background: Lactoferrin (Lf) is a glycoprotein, a member of the transferrin family.From ten known mechanisms of anti-cancer chemoperotecive compounds, Lf alone, has six of these functions and inhibits cancer. In this study, the effect of lactoferrin purified from bovine colostrum was studied as an anti-cancer agent on esophageal cancer cell line. Materials and Methods: Bovine colostrum were ...
[ 22 ] - Blood groups: In Health and Diseases
ABO blood type antigens are polymorphic, inherited structures presented on the surface of red blood cells. Although ABO blood group antigens is the most important antigens in transfusion medicine, its main role is not clearly clarified. Correlation between ABO blood group and susceptibility to certain infectious and non-infectious diseases is a controversial issue. Since, lack of blood type ant...
[ 23 ] - Generation of CHO Stable Cell Line Overexpressing HER2: an In Vitro Model for Breast Cancer
Background: Breast cancer is the most common female malignancy and the leading cause of cancer mortality in women worldwide. The human epidermal growth factor receptor2 (HER2) is a transmembrane tyrosine kinase receptor that is usually overexpressed in human breast cancers. Stable cell lines heterogeneously overexpressing HER2 are highly required as in vitro models for breast cancer research. T...
[ 24 ] - Anticancer effect of lactoferrin on Gastric Cancer Cell Line AGS
Background: Lactoferrin is a glycoprotein with a molecular weight of 80 kDa, as a member of the transferring family. In recent investigations the important biological properties such as anti microbial, antiviral and anticancer activity of lactoferrin were studied. In the present study, the effect of antitumor induced by lactoferin was evaluated on stomach cancer cell AGS. Materials and Methods...
[ 25 ] - The potential of human-derived periodontal ligament stem cells to osteogenic differentiation: An In vitro investigation
Background: Periodontal ligament stem cells (PDLSCs) are considered as a type of mesenchymal stem cell that is beneficial target for numerous clinical applications in periodontal tissue regeneration therapy. Materials and Methods: This study examined the effects of dexamethasone (Dex) on human PDLSCs in vitro. PDLSCs obtained from the roots of patient’s teeth were cultured with Dex (0....
[ 26 ] - Association of FCγRIIA (CD32) polymorphism with susceptibility to brucellosis
Background: Brucellosis is the major bacterial zoonoses of global importance caused by Brucella spps. FCγRIIA receptor plays a central role in phagocytosis of IgG2-opsonized bacteria. FCγRIIA exhibits allelic polymorphisms with different capacities for binding IgG2 and phagocytosis. Cells expressing Fc γ RIIa-H131, bind more efficiently to complexes of IgG2 than those expressi...
[ 27 ] - Inflammation, a Key Factor in Cancer Ambush
Inflammatory condition is the consequence of defensive mechanism of immune system against viral and bacterial infection, tissue injury, UV radiation, stress and etc. Persistently acute inflammation leads to chronic phase which is characterized by production of pro-inflammatory mediators from T cells. These molecules (e.g. IL-6, TNF-&alpha, IL-1&beta and IL-17) are mostly pleiotropic cytokines i...
[ 28 ] - Minimal HER1 and HER2 expressions in CHO and HEK-293 cells cause them appropriate negative cells for HERs-related studies
Background: Understanding of cellular and molecular mechanisms involved in cell/tumor growth and progression has led to molecular-targeted therapy. HER1 and HER2 are two main oncogenic components of HER-receptor family considered as targets for cancer research and therapy. Reliable results of HER- related research and targeted therapies dependent upon cell line with known HER expression status....
[ 29 ] - Urinary neutrophil-gelatinase associated lipocalin is a more prognostic biomarker to distinguish antenatal hydronephrosis in neonates
Background: Routine diagnostic methods of Vesicoureteral reflux (VUR) are invasive and can cause exposure to radiation and may increase risk of urinary tract infections. Therefore, introducing reliable, non invasive methods might be more interested in pediatric nephrology. The objective of this perspective case control study was to evaluate the prognostic value of urinary neutrophil-gelatinase ...
[ 30 ] - A simplified protocol for producing Taq DNA polymerase in biology laboratory
Background: Taq DNA polymerase is a very important enzyme for molecular biological studies such as DNA amplification and DNA sequencing by the PCR. It is a standard enzyme that is used in 90% of molecular biology labs today. The aim of this study was to produce Taq DNA polymerase enzyme in E. coli by a reliable, practical, simple and low cost method. Materials and Methods: In this study, the T...
[ 31 ] - Resistance to HER2-targeted therapy
Production and approval of trastuzumab (Herceptin®) for the treatment of metastatic breast cancer (MBC) was a millstone in antibody-based targeted therapy in the cancer treatment. However, despite the early success in the clinical trials, trastuzumab failed to appreciate the initial attraction due to development of resistance to the drug. The majority of patients who benefit from the drug...
[ 32 ] - Over-Expression of Immunosuppressive Molecules, PD-L1 and PD-L2, in Ulcerative Colitis Patients
[ 33 ] - An Overview of the Epigenetic Modifications of Gene Expression in Tumorigenesis
The five leading causes of cancer-related deaths are lung (1,760,000 deaths), colorectal (862,000 deaths), stomach (783,000 deaths), liver (782,000 deaths), and breast (627,000 deaths) cancers. Epigenetic changes can alter chromatin compaction, leading to the regulation of geneexpression without changing the primary DNA sequence.Epigenetic mechanisms are normally involved incellular processes s...
[ 34 ] - Safety Evaluation of Nano Iron Zero Valente Green Synthesized: A Comparative Study
Objective(s): Nowadays, examining the toxicity of nanoparticles including the synthesized and functionalized iron nanoparticles using methods like green synthesis is highly considered, due to their increasing usage in various fields of medicine, biology, industrial, and pollution removal. Hence, in this study, the toxicity of the zero valent iron nanoparticles synthesized by plant-Myrtus commun...
[ 35 ] - Green synthesis of multifunctional silver nanoparticles using quercetin and their therapeutic potential
Objective(s): Active species used in bio-chemical for synthesizing nanoparticles is poly phenolic compounds. The ability of flavonoids (e.g. quercetin) to dissolve in water is low and the production of metallic nanoparticles from them in the aqueous medium is hard. Previous studies recommend that quercetin was not capable of reducing Ag+ to Ag0. The current research aimed at synthesizing querce...
[ 36 ] - Cytotoxicity Effect of Cold Atmospheric Plasma on Melanoma (B16-F10), Breast (MCF-7) and Lung (A549) Cancer Cell Lines Compared with Normal Cells
Background and purpose: Cancer is one of the major health challenges in the world. The efficacy of current treatments is low but their side effects are high. Cold atmospheric plasma (CAP) is a new modality for cancer treatment. This study aimed to compare the cytotoxicity effect of CAP on the cell line models of common cancers and normal cells. Materials and methods: In this experimental study...
[ 37 ] - Effect of Different Tapered Gutta Percha Cones in Apical Microleakage of Human Root Canal Obturation
Background and purpose: Gutta-percha is generally accepted as the main canal filler. Standard gutta-percha cones are available in 2-10% tapers. The purpose of this study was to compare apical microleakage of canals obturated with different tapers of gutta-percha, using the protein penetration technique. Materials and methods: In this study, 96 maxillary anterior teeth were selected and were pr...
[ 39 ] - Interleukin 6 but not Interleukin 8 Might be an Indicator of Multiple Sclerosis Progression from Relapse Remitting to Secondary Progressive Status
Background: Multiple Sclerosis (MS) is the most common chronic inflammatory disease of the white matter of the Central Nervous System (CNS). Proinflammatory cytokine network such as interleukin-6 (IL-6) has a role in the initiation of a destructive immune response in CNS and the progression of the disease. This study aimed to evaluate the comparative levels of IL-6 and IL-8 in MS patients and h...
[ 40 ] - Effect of Cold Atmospheric Plasma on Gene Expression and Methylation of Genes Involved in Colorectal Cancer
Background and purpose: Colorectal cancer (CRC) is ranked as the second most common cancer in men and the third most common cancer in women worldwide. Routine treatments have many side effects and little efficiency. The aim of this study was to investigate the effects of Cold Atmospheric Plasma (CAP) on epigenetic changes of some genes involved in CRC progression. Materials and methods: Prolif...
[ 41 ] - Immunoinformatics Design of a Multi-epitope-based Vaccine Against Colorectal Cancer
Background: Bioinformatic approaches for designing vaccines have become a promising alternative to conventional methods. We herein designed a multi-epitope-based vaccine against colorectal cancer (CRC). Methods: Used peptides in the CRC vaccines were retrieved from databases of PubMed, Web of Science, Google Scholar, and Clinical trials. The adjuvants of Mycobacterial heparin-binding hemagglut...
Co-Authors
Reza Valadan 79