Mohammad Hassan Zuhair
Department of Immunology, School of Medicine, Tarbiat Modares University, Tehran, Iran
[ 1 ] - Evaluation of the Effect of the 47 kDa Protein Isolated from Aged Garlic Extract on Dendritic Cells
Objective(s) Garlic (Allium sativum) is known as a potent spice and a medicine with broad therapeutic properties ranging from antibacterial to anticancer, and anticoagulant. One of the major purified garlic protein components is the 47 kDa protein. In this study, the effect of 47 kDa protein extracted from aged garlic (AGE) was evaluated on mouse dendritic cell (DC) maturation in vitro. Mate...
[ 2 ] - Effect of Cell Wall, Cytoplasmic Fraction and Killed-Candida albicans on Nitric Oxide Production by Peritoneal Macrophages from BALB/c Mice
Objective(s) The fractions of Candida albicans have been used as an immunomodulator. The present work assessed the effect of different fractions of C. albicans on nitric oxide (NO) production by mice peritoneal macrophages. Materials and Methods Cell wall and cytoplasmic fractions of C. albicans ATCC 10321 strain were extracted. Mice peritoneal macrophages were purified and cultured. Differen...
[ 3 ] - Tumor Associated Mesenchymal Stromal Cells Show Higher Immunosuppressive and Angiogenic Properties Compared to Adipose Derived MSCs
Background: Differentiation, migratory properties and availability of Mesenchymal Stromal Cells (MSC) have become an important part of biomedical research. However, the functional heterogeneity of cells derived from different tissues has hampered providing definitive phenotypic markers for these cells. Objective: To characterize and compare the phenotype and cytokines of adipose derived MSCs (...
[ 4 ] - Arteether Exerts Antitumor Activity and Reduces CD4+CD25+FOXP3+ T-reg Cells in Vivo
Background: Chemo-immunotherapy is one of the new achievements for treatment of cancer, by which the success of anti-cancer therapy can be increased. In vitro studies have been shown that Arteether (ARE) induces apoptosis in tumor cells, but not in normal cells. Objective: To investigate the cytotoxic and immunomodulatory properties of Arteether in-vivo and in-vitro. Methods: In this study, we ...
[ 5 ] - Long Acting Propranolol and HSP-70 Rich Tumor Lysate Reduce Tumor Growth and Enhance Immune Response against Fibrosarcoma in Balb/c Mice
Background: Noradrenaline (NA), the principal neurotransmitter released from sympathetic nerve terminals, influences T-cell maturation, not only directly in developing T cells, but also indirectly, by acting on the thymic nonlymphoid cells. In vitro and in vivo studies have demonstrated the anti-proliferative, anti-migratory, antiangiogenic and cytotoxic properties of propranolol, β-AR blocker,...
[ 6 ] - Tehranolide Could Shift the Immune Response towards Th1 and Modulate the Intra-Tumor Infiltrated T Regulatory Cells
Background: Artemisia diffusa contains a new type of sesquiterpene lactone with an endoperoxide group (Tehranolide). Objective: Due to the existing similarity between the structures of Tehranolide and Artemisinin, it was hypothesized that Tehranolide would have similar effects as Artemisinin. In this study, the immunotherapeutic effec-tiveness of Tehranolide was investigated by direct intra-tum...
[ 7 ] - The Modulatory Effects of Vitamin D on the Expression of IL-12 and TGF-β in the Spinal Cord and Serum of Mice with Experimental Autoimmune Encephalomyelitis
Background & objective The immunoregulatory effects of transforming growth factor (TGF)-βand interleukin-12 (IL-12) and immunomodulatory actions of vitamin D (VD) were reported in several studies. This study aims to evaluate VD effects on IL-12 and TGF-β expression in experimental autoimmune encephalomyelitis (EAE). Methods:</str...
[ 8 ] - Evaluation of Association Between the Serum Levels of MMP-9 and MMP-9/TIMPs With Soluble Forms of Selectins and Itching Induced by Sulfur Mustard
Background & objective: Pruritus is the most frequent chronic dermal complication of sulfur mustard (SM), which negatively influences the quality of life. Exact pathophysiology of SM-induced itching is unknown. The current study aimed at evaluating the possible association between SM-induced itching and the serum levels of matrix metalloproteinase (MMP...
[ 9 ] - Comparative Analysis of CD4+ and CD8+ T Cells in Tumor Tissues, Lymph Nodes and the Peripheral Blood from Patients with Breast Cancer
Background: CD4+ and CD8+ T cells are the main types of lymphocytes in cell-mediated immunity and play a central role in the induction of efficient immune responses against tumors. The frequencies of T cell subtypes in the peripheral blood and tumor tissues, and draining lymph nodes (dLN) can be considered as useful markers for evaluation of the immune system in cancers. Methods: In this study,...
[ 10 ] - مقایسه عملکرد سیستم ایمنی سویه های آرین، بومی آذربایجان غربی، آمیخته های آنها و سویه راس
هدف از این مطالعه مقایسۀ عملکرد سیستم ایمنی جوجههای آرین، بومی آذربایجان غربی، آمیخته آرین × بومی، آمیخته بومی × آرین و سویۀ راس 308 بود. با انجام تلقیح مصنوعی تعداد 298 پرنده تولید و به همراه 100 پرنده سویه راس پرورش داده شدند. سپس، به طور تصادفی تعداد 15 تا 20 درصد پرندهها برای مقایسۀ عملکرد سیستم ایمنی و برخی پارامترهای خونی انتخاب شدند. صفات مورد مطالعه شامل پاسخهای ایمنی همورال، ایمنی...
[ 11 ] - Effect of Pulse-Modulated GSM-900 MHz Electromagnetic Field on the Electrochemotherapy Efficacy of 4T-1 Cells
Introduction: Electrochemotherapy (ECT) is a cancer treatment modality to permeabilize cell membrane facilitating the non-permeant molecules to gain access to the cytosol of cells. Nevertheless, environmental electromagnetic fields (EMFs) may disturb the efficiency of ECT. The present study aimed to investigate the effect of EMFs 900 MHz pulse-modulated by 217 Hz extremely low-frequency fields ...
[ 12 ] - Th1 Immune Response Induction by Biogenic Selenium Nanoparticles in Mice with Breast Cancer: Preliminary Vaccine Model
Background: Tumor associated antigens can be viably used to enhance host immune response. Objectives: The immunomodulatory effect of biogenic selenium nanoparticles (SeNPs) was compared between treated and untreated mice with crude antigens of 4T1 cells. Materials and Methods: Female inbred BALB/c mice (60) were injected by cancinogenic 4T1 cells causing breast cancer. After 10 days, all tumor ...
[ 13 ] - Evaluation of synergistic therapeutic effect of shark cartilage extract with artemisinin and glucantime on visceral leishmaniasis in BALB/c mice
Objective(s): Because leishmaniasis is related to the impaired functioning of T-cells, the use of an immunomodulator can increase the efficacy of antileishmanial therapy in visceral leishmaniasis. In this study, we used shark cartilage extract with artemisinin and glucantime against visceral leishmaniasis in BALB/c mice, and evaluated the synergistic therapeutic effect. Materials and Methods: Th...
[ 14 ] - Anti-cancer and anti-immunomodulatory properties of novel Arteether in Folic acid-Chitosan-Fe3O4 composite nanoparticle for treatment of breast cancer
Goal: The potent anti-cancer activity of Arteether (ARE) has been the focus of many studies. However, the hydrophobic property of this drug limits its application. To increase the bioavailability of ARE, we formulated a nanosystem (NS) of folic acid (FA), chitosan (CS) and Fe3O4 for delivery of ARE against breast cancer. Material and Methods: The CS coated Fe3O4 was synthesized by co-precipitat...
[ 15 ] - The Cell Survival of F10B16 Melanoma and 4T1 Breast Adenocarcinoma Irradiated to Gamma Radiation Using the MTT Assay Based on Two Different Calculation Methods
Background: Various MTT assay methods are proposed to obtain the cell sur- vival parameters.Objective: Determining the survival curve characteristics of two cancerous cells of interest based on a common and a novel MTT assay method after exposing them to ionizing radiation.Method: A common and a novel MTT assay method were used and compared for obtaining the F10B16 melanoma and 4T1 breast adeno...
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