Mohammad Hossein Nasr-Esfahani
Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran|Department of Molecular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran|Isfahan Fertility and Infertility Center, Isfahan, Iran
[ 1 ] - Diagnosis of genetic defects through parallel assessment of PLCζ and CAPZA3 in infertile men with history of failed oocyte activation
Objective(s): Phospholipase C ζ (PLCζ) is considered as a nominee for sperm associated oocyte activating factors and is located back-to-back with CAPZA3, an actin-capping protein controlling actin polymerization during spermiogenesis. They contain a common bidirectional promoter. The objective of this study was to identify individuals with parallel low expression of PLCζ and CAPZA3 mRNA, in hop...
[ 2 ] - روشی ساده و کاربردی برای دسترسی به نخاع ناحیه سینه ای در موش صحرایی
زمینه و هدف: آسیب های نخاعی به عنوان یکی از حوضه های مهم تحقیقات در علوم اعصاب مطرح می باشد . دسترسی به نخاع بدون ایجاد آسیب به آن، را می توان به عنوان فصل مشترک ایجاد مدل های مختلف ضایعه نخاعی در نظر گرفت . در این میان، قسمت تحتانی نخاع سینه ای بدلیل استقرار مراکز مرتبط با ایجاد الگو های حرکتی اندام تحتانی و همچنین وجود تست های استاندارد بررسیهای حرکتی، مورد توجه محققین به این ناحیه خاص شده ...
[ 3 ] - مقایسه بیان پروتئین PLCζ و آسیب DNA اسپرم بین مردان بارور و نابارور
Introduction: Sperm-specific phospholipase C zeta (PLCζ) protein is expressed during spermatogenesis and plays a main role during oocyte activation. In this study, the expression of sperm PLCζ protein and DNA damage in fertile and infertile men was assessed. Methods: In this case-control study, semen samples were collected from 15 fertile and 10 infertile men candidated for intra-cyt...
[ 4 ] - استرس اکسیداتیو و اثرات آن در ناباروری مردان: یک مقاله مروری
Background and Objectives: Reactive oxygen species (ROS) are essential for sperm physiological functions including capacitation, acrosome reaction, fertilization, and etc. Sperm cell in comparison with other cells is highly prone to oxidative attack due to high level of membrane unsaturated fatty acids and low volume of cytoplasmic space. Therefore, the aim of this review article was to underst...
[ 5 ] - Methylation Status of H19/IGF2 Differentially Methylated Region in in vitro Human Blastocysts Donated by Healthy Couples
Backgrund: Imprinted genes are a unique subset of few genes, which have been differentially methylated region (DMR) in a parental origin-dependent manner during gametogenesis, and these genes are highly protected during pre-implantation epigenetic reprogramming. Several studies heve shown that the particular vulnerability of imprinting genes during suboptimal pre- and peri-conception microenvir...
[ 6 ] - اضافه کردن کوآنزیم Q10 به محیط بلوغ، از طریق بهبود عملکرد میتوکندریایی، شایستگی تکوینی تخمک گوسفند را ارتقا می‌دهد
دست ورزی آزمایشگاهی گامت و جنین‌ها و همچنین سن بالای مادر می‌تواند تعادل بین ظرفیت آنتی اکسیدانی و رادیکال‌های آزاد را به نفع استرس اکسیداتیو جابجا کند. افزایش استرس اکسیداتیو می‌تواند باعث کاهش تولید انرژی و نقص در جدایی کروموزوم‌ها گردد و از این طریق تکوین رویان را به مخاطره بیاندازد. اضافه کردن کوآنزیم Q10 (CoQ10) به محیط می‌تواند باعث ایجاد تعادل در تولید انرژی و کاهش استرس اکسیداتیو گردد. ...
[ 7 ] - Analysis of Promyelocytic Leukemia in Human Embryonic Carcinoma Stem Cells During Retinoic Acid-Induced Neural Differentiation
Background: Promyelocytic leukemia protein (PML) is a tumor suppressor protein that is involved in myeloid cell differentiation in response to retinoic acid (RA). In addition, RA acts as a natural morphogen in neural development. Objectives: This study aimed to examine PML gene expression in different stages of in vitro neural differentiation of NT2 cells, and to investigate the possible role o...
[ 8 ] - تاثیر استرس گرمایی بر روند اسپرماتوژنز
زمینه و هدف: اسپرماتوژنز شامل یک سری از مراحل پیچیده است که در تمایز اسپرماتوگونی ها به اسپرم های بالغ دخالت دارد. روند تکوین اسپرماتوگونی ممکن است تحت تأثیر عوامل مختلف ازجمله: تنش گرمایی قرار گیرد و باعث کاهش کیفیت اسپرم و درنتیجه تأثیر منفی بر روی باروری گردد. به سختی می توان به طور کامل از عوامل افزایش دهنده دمای کیسه بیضه (از شیوه زندگی، شغل، محیط زیست تا شرایط پاتوفیزیولوژیکی) جلوگی...
[ 9 ] - نقش پروتئین PAWP اسپرم در فرایند اسپرماتوژنز و تکوین اولیه جنین
زمینه و هدف: در حال حاضر، مطالعه مقالات متعدد از این مسئله حمایت می کنند که نوسانات کلسیم سیتوزولی در تخمک توسط فاکتور (فاکتورهای) اسپرمی فعال کننده تخمک میانجیگری می شود که پس از فیوژن اسپرم با غشا تخمک، به داخل تخمک رها می‌شوند. فسفولیپاز C زتا به‌عنوان مهم‌ترین فاکتور درگیر در فعال شدن تخمک در نظر گرفته شده است؛ اما مطالعات اخیر، پیشنهاد می‌کنند که پروتئین متصل شونده به دامنه ی WW صفحات خل...
[ 10 ] - نقش پروتئین فسفولیپاز C زتا در باروری مردان به عنوان یک فاکتور فعال کننده تخمکی
زمینه و هدف: در پستانداران، اسپرم پس از ورود به تخمک به هنگام لقاح، یک آبشار سیگنالی را که شامل نوسانات در غلظت کلیسم آزاد سیتوپلاسمی تخمک است، ایجاد می‌کند. هدف از انجام این مطالعه مروری، بررسی نقش پروتئین PLCζ به عنوان یک فاکتور فعال کننده تخمکی و سپس نقش آن در نمونه اسپرم مردان بارور و نابارور می‌باشد. روش بررسی: در این مقاله مروری، 51 مقاله مرتبط با پروتئین PLCζ با استفاده از کلمات کلیدی ا...
[ 11 ] - تاثیر تمرین استقامتی و رژیم غذایی پرچرب بر بیان ژن Pgc1α وUcp1 در بافت چربی سفید زیرپوستی و چربی قهوه ای در موش‌های نر C57BL/6
هدف از این پژوهش بررسی اثر 8 هفته تمرین استقامتی و رژیم غذائی بر بیان ژن Pgc1α و Ucp1 در بافت چربی موش‌های نر چاق و نرمال C57BL/6 می‌باشد. 48 سر موش نر C57BL/6 به صورت تصادفی به دو گروه تقسیم شدند: 1. گروه تغذیه شده با رژیم غذایی پرچرب، 2. گروه تغذیه شده با رژیم غذایی کم چرب (به مدت 12 هفته). سپس هر دو گروه چاق و نرمال براساس میانگین وزنی برابر به چهار گروه تقسیم شدند: رژیم غذایی پرچرب-تمرین (H...
[ 12 ] - تأثیر تمرین استقامتی و رژیم غذایی پرچرب بر مسیر PGC-1a/FNDC5/Irisin در موش‌های نر C57BL/6
هدف مطالعة حاضر، بررسی تأثیر تمرین استقامتی و رژیم غذایی پرچرب بر مسیر PGC-1a/FNDC5/Irisin در بافت عضلة دوقلو و بافت چربی احشایی در موش‌های نر C57BL/6 بود. موش‌ها به‌طور تصادفی به چهار گروه تقسیم شدند: رژیم غذایی پرچرب- تمرین (HF-E)، رژیم غذایی پرچرب- بی‌تحرک (HF-S</str...
[ 13 ] - Relationship between Expression of Sperm PAWP Protein with Fertilization Rate in Infertile Men Candidate for ICSI Technique
Backgroundm: Post-acrosomal sheath WW domain binding protein (PAWP) is one of the proteins that is expressed during spermatogenesis process and has several roles in sperm differentiation, fertilization, and early embryonic development. The aim of this study was assessment of the relationship between PAWP expression with fertilization rate in infertile men candidate for intracytoplasmic sperm...
[ 14 ] - The role of Musashi protein in spermatogenesis and male infertility
Background: Inactivation of transcription occurs during two phases of spermatogenesis. First, in spermatocytes entering the primary meiosis and the second in round and elongating spermatids. These stages of inactivated transcription demand extensive regulation of translation. Therefore, presence of the control on gene expression during spermatogenesis seems essential. In the cases that post-tra...
[ 15 ] - Evaluation of comparison of clinical outcome treated oocyte with ionomycin and strontium chloride in infertile couple with previous failed in vitro fertilization
Introduction: Several factors are involved in failed fertilization following intracytoplasmic sperm injection (ICSI), which could be related to sperm, oocyte factors and/or both. Failure in oocyte activation is considered as the most important factor in failed fertilization after ICSI. To overcome this shortcoming, artificial oocyte activation (AOA) after ICSI has been suggested. Commonly, iono...
[ 16 ] - Comparison of the Effect of Alpha Lipoic Acid (ALA) and Beta-Defensin 1 Protein on Human Sperm Motility and Viability in Different Times of Incubation
Background and Objectives: Antioxidant alpha lipoic acid and β-defensin 1 protein may have a positive effect on the quality of sperm parameters. In this study, the effect of sperm incubation with alpha lipoic acid antioxidant and β-defensin 1 protein were assessed on sperm motility and viability at different times. Methods: In this experimental study, routine semen analysis was carried out o...
[ 17 ] - نقش و عملکرد تلومر در تولید مثل
اهداف: تلومر‌ها ساختار‌های حفاظت شده‌ی هتروکروماتینی غیر کد شونده با تکرار‌های شش نوکلئوتیدی غنی از گوانین هستند که در انتهای کروموزوم‌ها قرار دارند. این ساختار در طی تکامل حفظ شده‌اند و عملکرد آن ها به طول ساختار وابسته است. در سلول‌های سوماتیک، طول تلومر در هر بار تقسیم سلولی کاهش می‌یابد. کوتاه شدن بیش از حد طول تلومر باعث توقف در چرخه‌ی سلولی، ناهنجاری در تفکیک شدن کروموزوم‌ها و مرگ سلولی...
[ 18 ] - Effect of alpha-lipoic acid antioxidant on sperm parameters and treatment of infertile men
Background: Today, infertility is a problem that affects about 15% of couples due to male factors, female factors or both. High levels of oxidative stress, because of an imbalance between the reactive oxygene species and the antioxidant capacity, have been repeatedly reported in infertile men, which can have a destructive effect on the spermatogenesis process. Antioxidant treatment can improve ...
[ 19 ] - Comparison of Sperm Telomere Length between Two Sperm Selection Procedures: Density Gradient Centrifugation and Zeta Potential
Background: Telomeres are particular sequences of DNA located at the end of the eukaryotic chromosomes that are essential for genome integrity. Telomere length in spermatozoa differs among males, as well as spermatozoa. Also, decreased telomere length in spermatozoa of infertile men is associated with the reduction of fertility potential and embryo quality. Density gradient centrifugation (DGC)...
[ 20 ] - Micronutrients in Support to The Carbon Cycle Activate Antioxidant Defences and Reduce Sperm DNA Damage in Infertile Men Attending Assisted Reproductive Technology Programs: Clinical Trial Study
Background: Micronutrients in support to the carbon cycle were shown to reduce sperm DNA damage both in animal models and infertile men. Besides supporting DNA methylation, their positive effect may be mediated by an improved performance of the endogenous antioxidant system but this has not yet been proven in clinical settings. The present study aimed at evaluating the effects of micronutrient ...
[ 21 ] - Relationship between Sperm Parameters with Sperm Function Tests in Infertile Men with at Least One Failed Cycle after Intracytoplasmic Sperm Injection Cycle
Objective Imbalance between production of reactive oxygen species (ROS) and total antioxidant capacity in testis, epididymis, and seminal fluid can eventually lead to subfertility and infertility. Abnormal chromatin packaging, and DNA fragmentation is considered as the main underlying causes. In this regard, we aimed to assess relationship between sperm parameters with DNA damage, protamine def...
[ 22 ] - Effect of Vitamin D Supplementation on Intracytoplasmic Sperm Injection Outcomes: A Randomized Double-Blind Placebo-Controlled Trial
Objective Despite numerous studies indicating an imperative role for reproduction, however, the role of Vitamin D supplementation on outcomes of assisted reproductive techniques remains controversial. Therefore, the objective of this clinical trial was to evaluate the effect of consumption of Vitamin D supplementation 6 weeks prior to ICSI on fertility indexes. MaterialsAndMethods The present s...
[ 23 ] - Dietary Patterns and The Outcomes of Assisted Reproductive Techniques in Women with Primary Infertility: A Prospective Cohort Study
Objective Infertility is one of the most common challenges that women of reproductive age would encounter today. The maternal nutritional status is a particularly important determinant of oocyte quality and embryonic growth. Studies on whether the overall diet can affect reproductive outcomes in infertile women are few. So this study was conducted to assess the relationship between dietary patt...
[ 24 ] - Chances to have a boy after gender selection by pre-implantation genetic diagnosis are reduced in couples with only girls and without a boy sired by the male partner
Objective Gender selection and family planning have their roots in human history. Despite great interest in these fields, very few scientific propositions exist which could explain why some family do not attain the desired sex. Therefore, the aim of this study was to evaluate whether sex of previous child or children could affect the outcomes of pre-implantation genetic diagnosis (PGD). Materia...
[ 25 ] - A Preliminary Study: N-acetyl-L-cysteine Improves Semen Quality following Varicocelectomy
a:4:{s:9:"Objective";s:680:"Surgery is considered the primary treatment for male infertility from clinical varicocele. One of the main events associated with varicocele is excessive production of reactive oxygen species (ROS). N-acetyl-L-cysteine (NAC), an antioxidant that scavenges free radicals, is considered a supplement to alleviate glutathione (GSH) depletion during oxidative stress. Despi...
[ 26 ] - Zeta Sperm Selection Improves Pregnancy Rate and Alters Sex Ratio in Male Factor Infertility Patients: A Double-Blind, Randomized Clinical Trial
Objective Selection of sperm for intra-cytoplasmic sperm injection (ICSI) is usually considered as the ultimate technique to alleviate male-factor infertility. In routine ICSI, selection is based on morphology and viability which does not necessarily preclude the chance injection of DNA-damaged or apoptotic sperm into the oocyte. Sperm with high negative surface electrical charge, named “Zeta p...
[ 27 ] - I-14: Novel Concepts in Molecular Pathology May Open A New Era in Treatment of Clinical Varicocele
Background Despite the long history associated with varicocele, it remains one of the most controversial issues in the field of Andrology. The main base of this is our current understanding of the pathophysiology of this disease. This has hampered treatment and management of varicocele, especially regarding why, when and to whom varicocelectomy should be applied. The main molecular pathology of...
[ 28 ] - I-15: Assessment of Transcript and Protein Profiles of Infertile Individual May Help to Select Individuals with Low Fertilization Potential Candidate of Artificial Oocyte Activation
Background Following sperm penetration, oocyte is activated by sperm oocyte activating factors (SOAFs) released by sperm. Spermspecific phospholipase C isoform ζ (PLCζ) and post acrosomal WW binding protein (PAWP) are two nominees for the SOAF. PLCζ is located back-to-back with another testis-specific gene called CAPZA3. These two genes share a common bidirectional promoter. In this study we as...
[ 29 ] - P-17: Effect of Calcium Ionophore A23187 on Sperm Hyper-Active Motility Patterns
Background The role of calcium in physiological phenomenona such as sperm acrosome reaction and hyper-activation has been well known. Calcium ionophore A23187 is routinely used for induction of acrosome reaction in mammalians sperm. Therefore, we decided to evaluate the effect of calcium ionophore A23187 on the sperm hyper-active motility patterns. MaterialsAndMethods Ten semen samples from ind...
[ 30 ] - P-20: Efficiency of Magnetic-Activated Cell Sorting System in Separation of Normal Sperm
Background Sperm preparation before assisted reproductive techniques is very important for obtaining successful fertilization and pregnancy. During ICSI technique, sperms are selected based on viability and morphology. But this selection did not guarantee integrity of sperm DNA. Therefore, novel sperm selection procedures are introduced for selection of sperm with low DNA damage. One of these p...
[ 31 ] - P-56: Artificial Oocyte Activation Alone Intra Cytoplasmic Sperm Injection in Men with Severe Semen Parameters
Background Failed fertilization occurs 1-3% in the infertile couples. Failure of oocyte activation (OA) is mainly attributed to failed fertilization. ICSI alone artificial oocyte activation (AOA) is only treatment approach for these individuals. Three procedures are introduced for AOA: chemical, mechanical and electrical. Most of the clinical Centers have commonly used ionomycin for activation ...
[ 32 ] - The First Report of Successfully Pregnancy after ICSI with Combined DGC/Zeta Sperm Selection Procedure in a Couple with Eleven Repeated Fail IVF/ICSI Cycles
This is the first report implementing combined density gradient centrifugation/Zeta (DGC/Zeta) sperm selection procedure for a couple with eleven previous intra cytoplasmic sperm injection/ in vitro fertilization (ICSI/IVF) failure cycles. Semen analysis was carried out according to World Health Organization criteria. Protamine deficiency, DNA fragmentation and morphology were assessed by chrom...
[ 33 ] - Credibility of Chromomycin A3 Staining in Prediction of Fertility
Background Chromomycin A3 (CMA3) staining has been used to assess protamine deficiency. The aim of this study was to determine credibility of CMA3 along with semen parameters for assessment of fertility potential. MaterialsAndMethods Semen analysis and CMA3 staining were carried out on 234 fertile and 178 subfertile individuals. Semen analysis was assessed according to WHO criteria. Protamine d...
[ 34 ] - Single Nucleotide Polymorphism Analysis of Protamine Genes in Infertile Men
Background Single nucleotide polymorphism (SNPs) are considered as one of the underlying causes of male infertility. Proper sperm chromatin packaging which involves replacement of histones with protamines has profound effect on male fertility. Over 20 SNPs have been reported for the protamine 1 and 2. MaterialsAndMethods The aim of this study was to evaluate the frequency of two previously repo...
[ 35 ] - Effect of PolyVinyl Pyrrolidone on Sperm Membrane Integrity and Chromatin Status
Background The aim of present study is to evaluate the effect of PolyVinyl Pyrrolidone (PVP) routinly used during ICSI procedure on sperm membrane integrity, and sperm chromatin status. MaterialsAndMethods This study was carried out on 21 semen samples from the infertile men referring to Isfahan Fertility and Infertility Center. The processed semen samples were divided into two portions. One po...
[ 36 ] - Effects of Sperm Acrosomal Integrity and Protamine Deficiency on In Vitro Fertilization and Pregnancy Rate
Background The objective of this study was to evaluate the relationship between protamine deficiency, and acrosomal integrity with fertilization and pregnancy rate in patients undergone in vitro fertilization (IVF). MaterialsAndMethods Semen samples from 70 infertile couples undergoing IVF at Isfahan Fertility and Infertility center were assessed in this study. Semen analysis was carried out ac...
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