Bahram Kazemi

[ 1 ] - Selection of single-chain variable fragments specific for Mycobacterium tuberculosis ESAT-6 antigen using ribosome display

Objective(s): Tuberculosis (TB) is still one of the problematic infectious diseases in developing countries, especially in Iran. In the present study, we applied ribosome display technique to select single chain variable fragments (scFvs) specific for the 6-kDa early secretory antigenic target (ESAT-6) antigen of Mycobacterium tuberculosis from a mouse scFv library. Materials and Methods: The g...

[ 2 ] - Cloning, Expression and Purification of Truncated Chlamydia Trachomatis Outer Membrane Protein 2 (Omp2) and its Application in an ELISA Assay

Background: Although a simple and direct method does not exist for the detection of chlamydial infections, there are situations in which reliable serological tests, with sensi-tivity related to a specific antigen, could be helpful. Objective: The aim of this study was to clone the first 1100 bp of the C. trachomatis outer membrane protein 2 (omp2) gene in order to prepare a recombinant protein ...

[ 3 ] - Development of aptameric affinity ligands specific to human plasma coagulation factor VIII using SEC-SELEX

Protein specific aptamers are highly applicable affinity ligands in different fields of research and clinical applications. They have been developed against various targets, in particular, bio-macromolecules such as proteins. Among human proteins, the coagulation factors are the most attractive targets for aptamer selection and their specific aptamers had valuable characteristics in therapeutic...

[ 4 ] - SRL-coated PAMAM dendrimer nano-carrier for targeted gene delivery to the glioma cells and competitive inhibition by lactoferrin

Glioma, as a primary tumor of central nervous system, is the main cause of death in patients with brain cancer. Therefore, development of an efficient strategy for treatment of glioma is worthy. The aim of the current study was to develop a SRL peptide-coated dendrimer as a novel dual gene delivery system for targeting the LRP receptor, an up-regulated gene in both BBB and glioma cells. To perf...

[ 5 ] - Periplasmic Expression of TNF Related Apoptosis Inducing Ligand (TRAIL) in E.coli

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), a member of TNF family, is an interesting ligand which selectively induces apoptosis in tumor cells and, therefore, it has been developed for cancer therapy. This ligand has been produced by various hosts such as E.coli. However, protein expression in E.coli cytoplasm leads to problems such as incorrect folding, reduction in biolo...

[ 6 ] - Cloning and Expression of TNF Related Apoptosis Inducing Ligand in Nicotiana tabacum

Molecular farming has been considered as a secure and economical approach for production of biopharmaceuticals. Human TNF Related Apoptosis Inducing Ligand (TRAIL) as a promising biopharmaceutical candidate has been produced in different expression hosts. However, little attention has been paid to molecular farming of the TRAIL in spite of numerous advantages of plant expression systems. Theref...

[ 7 ] - Challenges to Design and Develop of DNA Aptamers for Protein Targets. I. Optimization of Asymmetric PCR for Generation of a Single Stranded DNA Library

Aptamers, or single stranded oligonucleotides, are produced by systematic evolution of ligands by exponential enrichment, abbreviated as SELEX. In the amplification and regeneration step of SELEX technique, dsDNA is conversed to ssDNA. Asymmetric PCR is one of the methods used for the generation of ssDNA. The purpose of this study was to design a random DNA library for selection of aptamers wit...

[ 8 ] - A study of hematological changes in sheep naturally infected with Anaplasma spp. and Theileria ovis: Molecular diagnosis

BACKGROUND: Ovine anaplasmosis and theileriosis are important tick-borne diseases of sheep and goats which are distributed in the tropical and subtropical areas of the world. OBJECTIVES: This study was performed to assess hematological status in sheep naturally infected with Anaplasma and Theileria spp. to clarify the pathogenic aspects of various species involved in ovine anaplasmosis and thei...

[ 9 ] - A convenient method to generate methylated and un-methylated control DNA in methylation studies

Methylated and un-methylated control DNA is an important part of DNA methylation studies. Although human and mouse DNA methylation control sets are commercially available, in case of methylation studies on other species such as animals, plants, and bacteria, control sets need to be prepared. In this paper a simple method of generating methylated and un-methylated control DNA is described. Whole...

[ 10 ] - Development of aptameric affinity ligands specific to human plasma coagulation factor VIII using SEC-SELEX

Protein specific aptamers are highly applicable affinity ligands in different fields of research and clinical applications. They have been developed against various targets, in particular, bio-macromolecules such as proteins. Among human proteins, the coagulation factors are the most attractive targets for aptamer selection and their specific aptamers had valuable characteristics in therapeutic...

[ 11 ] - SRL-coated PAMAM dendrimer nano-carrier for targeted gene delivery to the glioma cells and competitive inhibition by lactoferrin

Glioma, as a primary tumor of central nervous system, is the main cause of death in patients with brain cancer. Therefore, development of an efficient strategy for treatment of glioma is worthy. The aim of the current study was to develop a SRL peptide-coated dendrimer as a novel dual gene delivery system for targeting the LRP receptor, an up-regulated gene in both BBB and glioma cells. To perf...

[ 12 ] - Periplasmic Expression of TNF Related Apoptosis Inducing Ligand (TRAIL) in E.coli

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), a member of TNF family, is an interesting ligand which selectively induces apoptosis in tumor cells and, therefore, it has been developed for cancer therapy. This ligand has been produced by various hosts such as E.coli. However, protein expression in E.coli cytoplasm leads to problems such as incorrect folding, reduction in biolo...

[ 13 ] - Cloning and Expression of TNF Related Apoptosis Inducing Ligand in Nicotiana tabacum

Molecular farming has been considered as a secure and economical approach for production of biopharmaceuticals. Human TNF Related Apoptosis Inducing Ligand (TRAIL) as a promising biopharmaceutical candidate has been produced in different expression hosts. However, little attention has been paid to molecular farming of the TRAIL in spite of numerous advantages of plant expression systems. Theref...

[ 14 ] - Challenges to Design and Develop of DNA Aptamers for Protein Targets. I. Optimization of Asymmetric PCR for Generation of a Single Stranded DNA Library

Aptamers, or single stranded oligonucleotides, are produced by systematic evolution of ligands by exponential enrichment, abbreviated as SELEX. In the amplification and regeneration step of SELEX technique, dsDNA is conversed to ssDNA. Asymmetric PCR is one of the methods used for the generation of ssDNA. The purpose of this study was to design a random DNA library for selection of aptamers wit...

[ 15 ] - خالص‌سازی و شناسایی باند پروتئینی از سارکوسیستیس گوسفند

[ 16 ] - Molecular Cloning, Expression and Enzymatic Assay of Pteridine Reductase 1 from Iranian Lizard Leishmania

Background: Currently, there are no effective vaccines against leishmaniasis, and treatment using pentavalent antimonial drugs is occasionally effective and often toxic for patients. The PTR1 enzyme, which causes antifolate drug resistance in Leishmania parasites encoded by gene pteridine reductase 1 (ptr1). Since Leishmania lacks pteridine and folate metabolism, it cannot synthesize the pterid...

[ 17 ] - Insulin Receptor Gene Mutations in Iranian Patients with Type II Diabetes Mellitus

Background: Patients with diabetes mellitus type II suffer from hyperglycemia because they are not able to use the insulin that they produce, often due to inadequate function of insulin receptors. There are some evidences that this deficiency is inherited in a dominant autosomal manner and leads to the malfunction of the pancreatic beta cells resulting in insulin excretion disorders. In this st...

[ 18 ] - DNA بارکدینگ گونه‌های گل‌خورک‌ماهی Boleophthalmus dussumeri)، Periophthalmus waltoni و (Scartelaos teneus در سواحل استان بوشهر

گل‌خورک‌ماهیان از نظر مطالعات بیولوژیک و اکوتوکسیکولوژیک دارای اهمیت هستند و قابلیت معرفی به عنوان نشانگر زیستی برای پایش محیط‌زیست و بررسی اکوسیستم‌های گلی و مانگرو در نواحی گرمسیری و نیمه‌گرمسیری را دارند. برای شناسایی دقیق گونه‌های گل‌خورک موجود در آب‌های سواحل بوشهر از روش بارکدینک استفاده شد. این روش، قابلیت شناسایی سریع ویژگی‌های تاکسونومی گونه‌ها را دارد و اطلاعات فراوانی را در این زمینه...

[ 19 ] - فعال سازی سیستم ایمنی اولیه توسط siRNA

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[ 20 ] - Cloning and Expression of Influenza H1N1 NS1 Protein in Escherichia Coli BL21

Background:Influenza virus is globally pathogenic and it is usually associated with zoonotic respiratory diseases. This virus has caused a number of pandemics with a high mortality rate. The non-structural (NS1) protein of influenza A viruses is a non-essential virulence factor that has multiple accessory functions during viral infection. This protein is highly conservative. It has been shown t...

[ 21 ] - Heterologous Expression of Human IL-29 (IFN-λ1) in Iranian Lizard Leishmania

Background: Interferons with diffrent functions such as antiviral, antiproliferative and immunomodulatory actions are effctive <span style="font-varia...

[ 22 ] - مروری بر تولید و کاربرد آنتی بادی های منوکلونال تک زنجیره ای: scFv

Abstract Background: Since the development of monoclonal antibodies by hybridoma technology the use of antibodies in targeted therapy has been considered. ScFv (single-chain variable fragment) is a small fragment of monoclonal antibody which binds to its target, especially. The goal of this study, is introducing of scFv, its production pathways as well as its diagnostic and therapeutic appl...

[ 23 ] - بررسی تغییر ژن پروتئین M2 ویروس آنفلوانزا H1N1 در بیان آن درباکتری Ecoli سویه BL21

Aim and Background: By Attention to gene sequence and each amino acid codon in host which express protein, also in order to probable vaccine production against influenza A, a major cause of mortality in a year,this research was carried out. M2 protein is conserved and it can be a proper candidate vaccine for influenza infection. Materials and Methods: Present research was an experimental study....

[ 24 ] - The Effect of Human Recombinant Tumor Necrosis Factor Receptor-2 on Reducing Inflammatory of Collagen -Induced Arthritis in Balb/c Mice

Background: The tumor necrosis factor alpha (TNFα) is a cytokine that produced principally by monocyte/macrophages and T lymphocytes, respectively. TNFα is recognized as the primary mediator of immunity in inflammation reaction.  One important application of Tumor Necrosis Factor Receptor 2 (TNFR2) is for the treatment of autoimmune diseases like rheumatoid arthritis (RA).<br /...

[ 25 ] - In-vitro Transcribed mRNA Delivery Using PLGA/PEI Nanoparticles into Human Monocyte-derived Dendritic Cells

Induction of protein synthesis by the external delivery of in-vitro transcription-messenger RNA (IVT-mRNA) has been a useful approach in the realm of cell biology, disease treatment, ‎reprogramming of cells, and vaccine design. Therefore, the development of new formulations for ‎protection of mRNA against nucleases is required to maintain its activity in-vivo. It was the aim o...

[ 26 ] - Designing an Engineered Construct Gene Sensitive to Carbohydrate In-vitro and Candidate for Human Insulin Gene Therapy In-vivo

Background and Aim: Diabetes is a common disorder worldwide, and exhaustive efforts have been made to cure this disease. Gene therapy has considered as a potential curative method that has more stability in comparison with the other pharmaceutical methods. However, the application of gene therapy as a definitive treatment demands further investigation. This study aim is to prepare a suitable hi...

[ 27 ] - In-vitro Transcribed mRNA Delivery Using PLGA/PEI Nanoparticles into Human Monocyte-derived Dendritic Cells

Induction of protein synthesis by the external delivery of in-vitro transcription-messenger RNA (IVT-mRNA) has been a useful approach in the realm of cell biology, disease treatment, ‎reprogramming of cells, and vaccine design. Therefore, the development of new formulations for ‎protection of mRNA against nucleases is required to maintain its activity in-vivo. It was the aim o...

[ 28 ] - Designing an Engineered Construct Gene Sensitive to Carbohydrate In-vitro and Candidate for Human Insulin Gene Therapy In-vivo

Background and Aim: Diabetes is a common disorder worldwide, and exhaustive efforts have been made to cure this disease. Gene therapy has considered as a potential curative method that has more stability in comparison with the other pharmaceutical methods. However, the application of gene therapy as a definitive treatment demands further investigation. This study aim is to prepare a suitable hi...

[ 29 ] - کلون سازی مولکولی ژن های luxA وluxB باکتری Vibrio fischeri

پرتوافکنی زیستی در باکتری ها پدیده ای است که توسط آنزیم های لوسی فراز سازماندهی می گردد. این آنزیم ها به صورت هترودایمری متشکل از زیر واحدهای α و β هستند که توسط ژن های luxA و luxB رمز شده و همراه با ژن های luxCDE رمز کننده پروتئین های مورد نیاز جهت سنتز سوبسترای آلدئیدی، اپران lux باکتریVibrio fischeri را تشکیل می دهند. در تحقیق حاضر ناحیه کروموزومی واجد ژن های luxA و luxB باکتری Vibri...