Seyed Davar Siadat
Departments of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran|Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran
[ 1 ] - Construction, expression, purification and characterization of secretin domain of PilQ and triple PilA-related disulfide loop peptides fusion protein from Pseudomonas aeruginosa
Objective(s): Infection with Pseudomonas aeruginosa has been a long-standing obstacle for clinical therapy due to the complexity of the genetics and pathogenesis, as well for widespread resistance to antibiotics, thus attaching great importance to explore effective vaccines for prevention and treatment. This paper focuses on the introduction of novel Pseudomonas aeruginosa type IV pili (T4P)-ba...
[ 2 ] - تعیین هویت عوامل اتیولوژیک عفونت‌های ناشی ازاکتینومایستال‌ها در بیماران مراجعه‌کننده به دانشکده بهداشت دانشگاه علوم پزشکی تهران
زمینه و هدف: اکتینومایستال به گروهی از باکتری‌های گرم مثبت گفته می‌شود که ازنظر مورفولوژی دارای ساختمانی رشته‌ای با قطری حدود یک میکرون هستند. در این گروه، باکتری‌هایی از خانواده اکتینومایسس، نوکاردیا و استرپتومایسس جای می‌گیرند. در این پژوهش، عوامل اتیولوژیک عفونت‌های ناشی از اکتینومایستال‌ها در بیماران مراجعه‌کننده به دانشکده بهداشت دانشگاه علوم پزشکی تهران تعیین شده است. مواد و روش کار: ای...
[ 3 ] - Development of a Novel Anti-Adhesive Vaccine Against Pseudomonas aeruginosa Targeting the C-terminal Disulfide Loop of the Pilin Protein
The type IV pili (T4P) is a major virulence factor of Pseudomonas aeruginosa (P. aeruginosa) that is associated with primary adhesion, biofilm formation and twitching motility. This study focuses on the introduction of a novel biologically active subunit vaccine derived from the disulfide loop (DSL) of P. aeruginosa pilin. We investigated the expression of the novel PilA in-frame with pET26a ve...
[ 4 ] - High Yield Overexpression, Refolding, Purification and Characterization of Pseudomonas aeruginosa Type B-Flagellin: An Improved Method Without Sonication
Pseudomonas aeruginosa as an opportunistic pathogen is a significant cause of acute and chronic infections in patients with compromised defenses. This bacterium is motile via a single polar flagellum made of polymerized flagellin subunits differentiated into two major serotypes: A and B. flagellin plays an important role as a virulence factor in the adhesion, colonization and invasion of P. aer...
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