Seyed Hamid Aghaee-Bakhtiyari
Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran|Department of Medical Biotechnology, Mashhad University of Medical Sciences, Mashhad, Iran
[ 1 ] - Cell-specific targeting by engineered M13 bacteriophage expressing VEGFR2 nanobody
Objective(s): Filamentous bacteriophage M13 was genetically engineered to specifically target mammalian cells for gene delivery purpose. Materials and Methods: A vascular endothelial growth factor receptor 2 (VEGFR2)-specific nanobody was genetically fused to the capsid gene III of M13 bacteriophage (pHEN4/3VGR19). A mammalian expression construct containing Cop-green fluorescent protein (Cop-G...
[ 2 ] - بهینه سازی و مقایسه سه حامل تجاری درترانسفکشن موثر به سلولهای تک هسته ای خون محیطی (PBMCs)
Background & Aim: Peripheral Blood Mononuclear Cells (PBMCs) are the main components of the immune system, including lymphocytes and monocytes. These cells are being used in most immunological studies and therefore, isolation, culture and transfection of these cells are of great importance. The transfection of these cells is currently a key concern; this is because most common methods of transf...
[ 3 ] - Investigating lipopolymers based on polyethylenimine and nanoliposome for gene delivery to prostate cancer (PC3) cell line
Background: Non-viral Nano carriers such as liposomes and cationic polymers based on engineered properties are regarded in gene delivery field. Although these carriers do not have weaknesses of viral vectors, but they are less efficient than viruses and they still need to be improved as favorable gene delivery carriers. Amongst non-viral carriers, cationic liposomes have been proposed for clini...
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