B. Shareghi
Department of Biology, University of Shahrekord, P.O. Box: 115, Shahrekord, I. R. of Iran.
[ 1 ] - Comparative Studies on the Interaction of Proteinase-K with Fe2O3, Fe3O4 and SiO2 Nanoparticles
The interaction of Fe2O3, Fe3O4 and SiO2 nanoparticles with proteinase K activity was investigated using UV–vis spectroscopy. Proteinase K EC (3.4.21.14) is a member of serine protease family, which is produced from fungus Tritirachum album Limber.The effects of nanoparticles on proteinase...
[ 2 ] - The Effect of TiO2-Nanoparticle on the Activity and Stability of Trypsin in Aqueous Medium
Trypsin (E.C.3.4.21.4) is a serine protease commonly used in proteomics for digestion of proteins. In the present study, the effect of nano-TiO2 on the conformation and catalytic activity of trypsin were studied. The thermal denaturation of trypsin has been investigated in the presence and absence of nano-TiO2 over the temperature range (293-373 K) at pH 3.0 and 7.25, using temperature scanning...
[ 3 ] - Cloning, Codon Optimization, and Expression of Yersinia intermedia Phytase Gene in E. coli
Background: Phytate is an anti-nutritional factor in plants, which catches the most phosphorus contents and some vital minerals. Therefore, Phytase is added mainly as an additive to the monogastric animals’ foods to hydrolyze phytate and increase absorption of phosphorus. Objectives: Y. intermedia phytase is a new phytase with special characteristics such as high specific activity, pH stabilit...
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