To Study the Changes of the Seminiferous Epithelium After Ga.Ai.As Laser Radiation (830Nm)

Purpose: The purpose of this study was to investigate the immediate effects of low power laser radiation (Ga-Al-As; 830nm) on the seminiferous epithelium of Sprague-Dawley rats. Materials and Methods: Male Sprague-Dawley rats were randomly divided into three groups (two experimental groups and the sham). The left testes of the animals were shaved, washed and marked at inferior pole. Then, they were exposed to laser light for 15 days in way that the cumulative doses used 28.05 J/cm2and 46.80J/cm2 in two experimental groups respectively. Sampling was carried out 24 hours after the last treatment. The samples were fixed in Bouins fixative and the slides were prepared for LM study. Besides evaluating the morphologic changes, the quantitative changes of the seminiferous epithelium were assessed by morphometric techniques. Results: 1. In the testes irradiated at 28.05J/cm2 the thickness, surface occupied by the epithelium, and the number of germ cells had increased. Whereas no change was observed in the morphology of the epithelium. 2. In the testes irradiated at 46.80 J/cm2, the thickness of epithelium had decreased and in most of the seminiferous tabules cleft formation and immature germ cell sloughing into the lumen of tubules was abserved. nevertheles in the lumens without germ cell releasing the proportion of the germ cells was similar to that of the sham. Conclusion: The results revealed that morphologic and morphometric changes of the seminiferous epithelim induced by Ga. AL. As laser radiation depend on the important facter that is the aplied dose; in the manner that the radiation with low doses has the stimulative effects on spermatogenesis whereas radiation with doses higher than biostimulative threshold has distructive effects on the epithelium and is followed by inhibition of spermatogenesis.