The Positive Effect of miR-1 Antagomir on Ischemic Neurological Disorders via Changing the Expression of bcl-w and Bad

MicroRNAs are non-coding RNAs. Studies have shown that miRNAs are expressed aberrantly in stroke. MiR-1 enhances ischemic damage, and previous study has demonstrated that reduction of miR-1 level has neuroprotective effect on Middle Cerebral Artery Occlusion(MCAO). Since apoptosis is one of the important process in neural protection, possible effect of miR-1 on this pathway has been tested in this study. Post-ischemic administration of miR-1 antagomir reduces infarct volume via bcl-w and Bad expression. Rats were divided into four experimental groups including sham, control, positive control, and antagomir treatment group. One hour after middle cerebral artery occlusion surgery (MCAO), rats were received intravenously (Tail vein) 0.1 ml normal saline (NS), 0.1 ml Rapamycin and 300 pmol/g miR-1 antagomir (Soluble in 0.1 ml normal saline) in control, positive control, and treatment group, respectively. Twenty four hours after reperfusion infarct volume was measured. The expression of miR-1, bcl-w, and Bad were analyzed using real time PCR in sham, control, and treated group. Our results indicate that administration of miR-1 antagomir reduces infarct volume significantly, it has also decreases miR-1 and Bad expression while increases bcl-w expression. Understanding precise neuroprotective mechanism of miR-1 antagomir can make it a proper treatment and  an innovative approach for stroke therapy.