The influence of enzymatic pre-treatment of corn or soybean meal on their phytate content under different in vitro conditions

Authors

  • Behzad Mansoori Department of Animal and Poultry Health and Nutrition, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • Jila Honarzad Department of Animal and Poultry Health and Nutrition, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
  • Mehrdad Modirsanei Department of Animal and Poultry Health and Nutrition, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • Mohammad Rezaeian Department of animal health and nutrition, Faculty of veterinary medicine, University of Tehran
  • Mohsen Farkhoy Department of Animal and Poultry Health and Nutrition, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
  • Zahra sadat Rahimi Department of Animal and Poultry Health and Nutrition, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Abstract:

BACKGROUND: Phosphorous is one of the expensive nutrients in poultry feed. Therefore, improving the bioavailability of this nutrient in feed ingredients could be effective for lowering the cost of feed. OBJECTIVE: This study was performed to evaluate the effect of pre-treatment of feed ingredients by commercial enzymes and different levels of pH on releasing of phosphorus from phytate under in vitro condition. METHODS: Three solutions including Distilled water, HCl 0.5% and HCl 1% (with pH=5.5, 2.12 and 2.12 respectively) and three enzymes (None, Bio-phytase, Rovabio Excel AP, and Rovabio Max AP) were used to determine phytate content of corn and soybean meal. First, each sample was supplemented with the enzymes and pre-treated under above mentioned solutions for 3 hours at 25 and 40ºC. RESULTS: The results indicated that pre-treating of corn samples with Bio-phytase or Rovabio Max AP and different solutions (at 25 ºC and 40 ºC for 3 hours) reduced phytate content significantly. The best results obtained with corn samples supplemented with Rovabio Max AP and mixing by HCl 1% at 25ºC, so that phytate content decreased up to 99.5% in comparison with control. The same results were also obtained for soybean samples. The highest reduction in phytate content (up to 47.4%) was observed by adding Rovabio Max AP and HCl 1% solution at 40ºC. CONCLUSION: It could be concluded that pre-treating of corn and soybean meal with several manners such as using commercial enzymes including phytase and solutions with different pH were effective to reduce phytate content that means increasing bioavailability of phosphorous.

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Journal title

volume 11  issue 4

pages  323- 335

publication date 2017-10-01

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