The Induction of Tolerance to Salinity Stress of Commercial Sugarcane Cultivar CP72-1312 by in Tissue Culture Condition

BACKGROUND: In order to investigate the induction of salinity tolerance in CP72-1312 commercial cultivar in tissue culture condition, an experiment was carried out in the tissue culture laboratory of Karoun Agro-Industry Company in 2017 year. OBJECTIVES: At this study we evaluated tolerance of CP73-1312 sugarcane commercial cultivar to salt stress in tissue culture. METHODS and RESULT: Six callus induction treatments in Murashig and Skoog based medium were studied in a CRD with 4 replications. MS based medium supplemented with 2,4-D in 3 mg.l-1 was the best treatment for callus induction (93.3%). The effect of different levels of salinity (0.2, 0.4, 0.6, 0.8 and 1 percent) on callus tolerance was studied in a CRD with 4 replications. In the eighth weeks, the callus value reduction in compare to control was obtained 23, 51, 66, 75 and 81 percent, respectively. Five regeneration treatments in modified MS were performed as factorial experiment in a CRD with 4 replications. The highest regeneration rate was 1 mg.l-1 BAP and 0.02 mg.l-1 KIN. This treatment was used for indirect regeneration under salinity stress condition. The mean comparisons showed that the highest regeneration rate was observed in the control treatment and the lowest regeneration was observed in salinity treatment 0.8 and 1 percent. Five root treatments in half-strength MS medium were studied in a CRD with 4 replications with IBA. The highest rooting rates were assigned to 0.01 and 0.02 mg.l-1 IBA treatments. The effect of the best level of IBA (0.01 mg.l-1) on salinity stress was evaluated on rooting. CONCLUSION: According to the results, with increasing salinity stress, rooting rate decreased.